英文名称phospho-B-Raf (Ser444)
中文名称 Anti-BLMH/博莱霉素水解酶抗体

别    名94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF 1; Braf; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; p94; RAFB 1; RAFB1; v raf murine sarcoma viral oncogene homolog B1; FLJ95109; BRAF_HUMAN.
规格0.1ml  
产品类型磷酸化抗体 
研究领域肿瘤  细胞生物  免疫学  信号转导  细胞凋亡  转录调节因子  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Rabbit, 
产品应用WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量94kDa
细胞定位细胞核 细胞浆 细胞膜 
性    状Lyophilized or Liquid
浓    度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human B-Raf around the phosphorylation site of Ser444
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Anti-BLMH/博莱霉素水解酶抗体he Raf kinases are important intermediates in signal transduction. Raf protein family members, including A Raf and B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase). Defects in BRAF are involved in a wide range of cancers. B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. 1 BRAF is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. BRAF acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones.

Function:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Subunit:
Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH.

Subcellular Location:
Nucleus. Cytoplasm. Cell membrane.

Tissue Specificity:
Brain and testis.

Post-translational modifications:
Phosphorylation at Ser-365 by SGK1 inhibits its activity.
Methylation at Arg-671 decreases stability and kinase activity. 
Ubiquitinated by RNF149; which leads to proteasomal degradation.

DISEASE:
Note=Defects in BRAF are found in a wide range of cancers. 
Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500]. 
Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis. 
Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. 
Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. 
Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hyperorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable inlectual deficits. 
Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hyperorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness. 
Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation.

Similarity:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. 
Contains 1 phorbol-ester/DAG-type zinc finger. 
Contains 1 protein kinase domain. 
Contains 1 RBD (Ras-binding) domain.

Gene ID:
673

Database links:
Entrez Gene: 673 Human
Entrez Gene: 109880 Mouse
Entrez Gene: 114486 Rat
Omim: 164757 Human
SwissProt: P15056 Human
SwissProt: P28028 Mouse
Unigene: 550061 Human
Unigene: 245513 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

BRAF蛋白是有丝分裂原活化的蛋白激酶/细胞外信号调节激酶途径蛋白。正常的B-Raf蛋白的功能是传递来自细胞膜的信号,在Ras-Raf-MEK-ERK信号转导调节途径中有着重要的作用。
BRAF是一种在癌细胞的生长和存活中起到关键作用的蛋白质,并且在大多数恶性黑素瘤患者和少数结肠癌、乳腺癌和肺癌患者中发生突变，目前BRAF也用于恶性黑素瘤和肿瘤的研究。

人参皂苷Rb1    Ginsenoside Rb1    HPLC≥98%    20mg/支    41753-43-9

人参皂苷Rb2    Ginsenoside Rb2    HPLC≥98%    20mg/支    11021-13-9
人参皂苷Rb3    Ginsenoside Rb3    HPLC≥98%    20mg/支    68406-26-8
人参皂苷Rg1    Ginsenoside Rg1    HPLC≥98%    20mg/支    22427-39-0
人参皂苷Rg2    Ginsenoside Rg2    HPLC≥98%    20mg/支    52286-74-5
人参皂苷Rg3    Ginsenoside Rg3    HPLC≥98%    20mg/支    14197-60-5
人参皂苷Rh1    Ginsenoside Rh1    HPLC≥98%    20mg/支    63223-86-9
人参皂苷Rh2    Ginsenoside Rh2    HPLC≥98%    20mg/支    78214-33-2
拟人参皂苷Rh2    Pseudoginsenoside Rh2    HPLC≥98%    5mg/支    1370264-16-6
人参皂苷Rh3    Ginsenoside Rh3    HPLC≥98%    20mg/支    105558-26-7
人参皂苷Rc    Ginsenoside Rc    HPLC≥98%    20mg/支    11021-14-0
人参皂苷Re    Ginsenoside Re    HPLC≥98%    20mg/支    52286-59-6
人参皂苷Rd    Ginsenoside Rd    HPLC≥98%    20mg/支    52705-93-8
人参皂苷Rf    Ginsenoside- Rf    HPLC≥98%    20mg/支    52286-58-5
拟人参皂苷RT5    Pseudoginsenoside-RT5    HPLC≥98%    20mg/支    98474-78-3
原人参三醇    Protopanaxatriol    HPLC≥98%    20mg/支    34080-08-5
(R型)人参皂苷Rh1    Ginsenoside Rh1    HPLC≥98%    20mg/支    
(R型)人参皂苷Rh2    Ginsenoside Rh2    HPLC≥98%    20mg/支    112246-15-8
(R型)人参皂苷Rg2    Ginsenoside Rg2    HPLC≥98%    20mg/支    80952-72-3
(R型)人参皂苷Rg3    Ginsenoside Rg3    HPLC≥98%    20mg/支    38243-03-7
(R型)原人参三醇    Protopanaxtriol    HPLC≥98%    20mg/支    1453-93-6
人参皂苷F1    Ginsenoside F1    HPLC≥98%    20mg/支    53963-43-2
人参皂苷F2    Ginsenoside F2    HPLC≥98%    20mg/支    62025-49-4
人参皂苷CK    Compound K    HPLC≥98%    20mg/支    39262-14-1
(R型)原人参二醇    Protopanaxdiol    HPLC≥95%    20mg/支    30636-90-9
人参皂苷Ro    Ginsenoside Ro    HPLC≥98%    20mg/支    34367-04-9
人参皂苷F3    Ginsenoside F3    HPLC≥98%    10mg/支    62025-50-7
人参皂苷Rg6    Ginsenoside Rg6    HPLC≥98%    20mg/支    
人参皂苷Rk1    Ginsenoside Rk1    HPLC≥95%    20mg/支    
人参二醇    Panaxadiol    HPLC≥98%    20mg/支    19666-76-3
人参三醇    Panaxtriol    HPLC≥98%    20mg/支    32791-84-7
原人参二醇    Protopanaxdiol    HPLC≥98%    20mg/支    30636-90-9
柴胡皂苷A    Saikosaponin A    HPLC≥98%    20mg/支    20736-09-8
柴胡皂苷C    Saikosaponin C    HPLC≥98%    20mg/支    20736-08-7
柴胡皂苷D    Saikosaponin D    HPLC≥98%    20mg/支    20874-52-6
柴胡皂苷B    Saikosaponin B    HPLC≥98%    20mg/支    
柴胡皂苷B1    Saikosaponin B1    HPLC≥98%    20mg/支    58558-08-0
柴胡皂苷B2    Saikosaponin B2    HPLC≥98%    20mg/支    58316-41-9
三七皂苷R1    Notoginsenoside R1    HPLC≥98%    20mg/支    80418-24-2
三七皂苷Ft1    Notoginsenoside Ft1    HPLC≥98%    20mg/支    
三七皂苷R2(R型)    20(S)-Notoginsenoside R2    HPLC≥94.50%    20mg/支    

Anti-ARK5     AMPK的相关蛋白激酶5抗体
Anti-ANP32C     致瘤磷蛋白32相关蛋白1抗体
Anti-AKIP     极光激酶A相互作用蛋白1抗体
Anti-ANKHD1     艾滋病病毒制约锚定蛋白抗体
Anti-Adenylate cyclase 1     腺苷酸环化酶1抗体
Anti-ABCE1     核糖核酸酶L抑制蛋白抗体
Anti-ARHGAP24     Rho GTP酶激活蛋白24抗体
Anti-ASAP3     GTP酶激活蛋白ASAP3抗体
Anti-ACTRIC/Activin A Receptor Type IC     激活素受体1C抗体
Anti-ADAMTS13     整合素样金属蛋白酶与凝血酶13型抗体
Anti-ADAM11   Anti-BHLHB9/p60TRP、p60TRP蛋白抗体
Anti-ADAM12/MLTN     去整合素样金属蛋白酶12抗体
Anti-ADAM15     去整合素样金属蛋白酶15抗体
Anti-ADAM2     去整合素样金属蛋白酶2抗体
Anti-ADA/Adenosine deaminase     腺苷脱氨酶抗体
Anti-ASB4     含锚蛋白重复序列-细胞因子信号抑制物盒蛋白家族4抗体
Anti-ADORA1/Adenosine A1 Receptor     腺苷A1受体抗体
Anti-ApoER2     载脂蛋白E2受体抗体
Anti-AQP0/MIP26/LIM1     水通道蛋白0抗体
Anti-AP2 gamma     转录因子AP-2γ抗体
Anti-ANGPTL5     血管生成素相关蛋白5
Anti-ANGPTL6     血管生成素相关蛋白6抗体
Anti-AMH/MIS     Muellerian缪勒管激素抑制因子抗体
Anti-EphA8/Eph receptor A8     酪氨酸蛋白激酶受体A8抗体
Anti-APOL6    载脂蛋白样蛋白6抗体
Anti-ARTS     凋亡相关蛋白TGFb信号抗体
Anti-ANKRD53     锚蛋白重复域53抗体
Anti-ANKRD54     锚蛋白重复域54抗体
Anti-AACS/ACSF1     脂肪酰辅酶A家族成员1抗体
Anti-A2LD1/GGACT     A2LD1蛋白抗体
Anti-A4GNT α1    4-N-乙酰葡糖胺转移酶α4Gn-T抗体
Anti-AADAC     芳香乙酰胺脱乙酰基酶抗体

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