技术文章

Anti-EpCAM上皮细胞粘附分子抗体-单克隆

上海研盟生物工程有限公司

2017/9/20 14:17:17

Mouse Anti-EpCAM antibody

 

Catalog Number : BYK-1513M       
Quantity size :   0.2ml
 (1mg/ml   dilute with Ph 7.4 0.01M PBS or antibody diluent)

 

Background: This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

 

Specificity :   

–  Anti-EpCAM is a mouse monoclonal antibody unconjugated                

–  specific for EpCAM of Human

–  use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–  Protein G affinity chromatography purification, purity :>95%

–  Isotype:  IgG

–  mol wt:  35kDa

 

Application :                                    

–  Western blotting=1:100-500

–  ELISA=1:500-1000

–  Immunohistochemistry =1:100-500

–  Optimal working dilutions must be determined by the end user.

 

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

 

Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.

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