文献名：P22 Viral Capsids as Nanocomposite High-Relaxivity MRI Contrast Agents
作者：Shefah Qazi †‡, Lars O. Liepold †‡, Md Joynal Abedin †‡, Ben Johnson †‡, Peter Prevelige ,Joseph A. Frank #, and Trevor Douglas *†‡
†Chemistry and Biochemistry Department and ‡Center for Bio-Inspired Nanomaterials, Montana State University, Bozeman, Montana, 59717, United States
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, United States
Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, and #National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, United States
摘要：Attachment of multiple chelated Gd3+ ions to the interior of bacteriophage P22 viral capsids affords nanoscale MRI contrast agents with extremely high relaxivity values. Highly fenestrated “wiffleball” morphology is unique to P22 and assures water exchange between the environment and interior cavity of the capsid. The cavity of P22 “wiffleball” was functionalized with a branched oligomer comprising multiple DTPA–Gd complexes resulting in an impressive payload of 1,900 Gd3+ ions inside each 64 nm capsid. High relaxivities of r1,ionic= 21.7 mM–1 s–1 and r1,particle = 41,300 mM–1 s–1 at 298 K, 0.65 T (28 MHz) are reported, withr1/r2 ratio of 0.80 and optimized rotational correlation time for this system. Specific design modifications are suggested for future improvements of viral capsid-based MRI contrast agents directed toward clinical translation.
关键词：viral capsid, VLP, bacteriophage P22, magnetic resonance imaging, MRI, imaging agent, contrast agent, T1, relaxivity, gadolinium, DTPA-Gd, azide-alkyne click chemistry, nanoparticle, nanocomposite, protein cages, payload
