起订量:
BioVisionK726-500 Lactate Dehydrogenase Activity Assay Kit
免费会员
代理商上海浩洋生物技术有限公司在为生命科学领域提供丰富的产品与信息资源方面处于全国*地位,公司提供的产品涵盖了二十多个国家近五十万种产品,而且产品的数量与信息在不断的增长和更新,公司提供的产品能够使生命科学工作者加快对生物化学,分子生物学,细胞生物学以及蛋白质组学研究的认知,以及分子诊断和临床医学领域的应用。
微信号: haoranbio
产品名称:Lactate Dehydrogenase Activity Assay Kit
产品价格:4940
产 地:BioVision
产品货号:K726-500
产品规格:500 assays
产品说明:
Storage Conditions: -20°C
Shipping Conditions: gel pack
Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.
• Species reactivity- Mammalian
• Application- The kit can detect 1 -100 mU/ml of LDH directly in samples.
Features & Benefits:
• Simple procedure; takes ~ 30 minutes
• Fast and convenient
• Sensitive assays for measuring LDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Kit components:
• LDH Assay Buffer
• LDH Substrate Mix (lyophilized)
• NADH Standard (0.5 µmol; lyophilized)
• LDH Positive Control
Description:
Lactate dehydrogenase (LDH) is an oxidoreductase (EC 1.1.1.27) present in a wide variety of organisms. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NADᶧ. When disease or injury or toxic material damages tissues, cells release LDH into the bloodstream. Since LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. In this colorimetric LDH quantification assay, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (λmax = 450 nm). The kit quantifies LDH activity in variety of biological samples such as in serum or plasma, cells, culture medium and fermentation, etc. The assay is quick, convenient, and sensitive. The kit can detect 1 -100 mU/ml of LDH directly in samples.