RF-O2荧光光纤氧气测量技术——氧气测量全面解决方案

    RF-O2荧光光纤氧气测量技术是基于REDFLASH光极传感器技术的氧气测量技术，由欧洲Pyroscience公司及Graz大学等科学家研制生产，由光极氧气传感器、测量仪及软件组成，广泛应用于环境科学、生态科学、植物科学、动物科学、海洋科学、生物医学、生物技术、食品科学等各个领域，其主要功能特点如下

1. REDFLASH光极氧气传感器技术，高精确度、高稳定性、高时空解析度、低能耗、无耗氧、无交叉敏感性
2. 传感器类型灵活多样，有探头式、探针式、非接触式（sensor spot）及纳米微粒式等，适应于液体和气体不同条件下的O2测量
3. 有内置sensor spot的流通管和呼吸瓶，非接触式测量流动液体的溶解氧及呼吸瓶内液体或气体中氧气含量
4. 轻便紧凑型FireStingO2测量仪，内置水汽、气压传感器，有1、2、4通道供选配，可分别接1个、2个、4个光极氧气传感器，另有Mini型FireStingO2-mini供选配
5. U盘式PiccolO2测量仪——世界上zui小的O2测量仪，可连接一个O2传感器，USB口连接电脑，即插即用

测量原理：

    REDFLASH光极O2传感器技术，利用*的O2敏感REDFLASH指示剂，通过610-630nm调制红光激发，REDFLASH指示剂发出760-790nm红外荧光，荧光强度随接触的O2分子浓度升高而发生荧光淬灭，这种荧光动态通过光纤传输到测量仪，测量仪灵敏地检测其相位漂移并据此换算成O2浓度

应用领域：

1. 水体溶解氧测量监测、藻类及藻类生物膜光合作用与呼吸作用测量监测
2. 植物光合作用与呼吸作用测量监测
3. 水生动物（鱼类、水生昆虫等无脊椎动物、浮游动物等呼吸代谢测量
4. 陆生动物、实验动物、动物组织、血液等呼吸代谢测量
5. 土壤、湿地、海洋沉积、河湖沉积剖面O2测量
6. 生物反应器、发酵过程、酶动力学、细胞培养等O2测量监测
7. 粮食食品储运、葡萄酒等O2测量监测
8. 污水处理、沼气、垃圾填埋场、有机物降解等O2测量监测

技术指标：

1. FireStingO2（FSO2）测量仪：
   1. 有1通道、2通道、4通道可供选配，分别可接1个、2个和4个O2传感器，可并联组成8通道甚至更多通道；另具备一个温度传感器通道（可选配4通道温度传感器）
   2. 激发光源620nm，监测器760nm（NIR）
   3. 采样频率：每秒4次
   4. 内置气压传感器，300－1100mbar，0.06mbar分辨率，精确度±3mbar
   5. 内置湿度传感器，0－100%，分辨率0.04%，精确度±0.2%
   6. 内置温度传感器，-40－125°C，分辨率0.01°C，精确度±0.3°C
   7. 具模拟输出和自动模式，0－2.5VDC
   8. USB接口，通过USB口PC供电
   9. 大小：68x120x30mm，重350g
2. PiccolO2 U盘式测量仪：大小仅15x15x54mm，重量约20g，单通道，激发光620nm，检测器760nm，采样频率每秒20次。可并联组成多通道测量系统。可通过PiccoTHP测量温湿度和气压并进行补偿

1. 探头式O2传感器：直径3mm，测量范围0-50%（0-23mg/l）（可选配其它范围），检测极限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精确度±0.2％（0.1mg/l）@20% O2，zui低使用寿命1千万数据点，存储时间大于3年（室温暗处储放）
2. 探针式O2传感器：有固定探针式、可伸缩探针式、尖头式及圆头式等不同类型供选配；探针直径有50μm、230μm、430μm等规格；测量范围0-50%（0-23mg/l）（可选配其它范围），检测极限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精确度±0.2％（0.1mg/l）@20% O2，zui快响应时间小于1s（与探针粗细有关），zui低使用寿命1百万数据点，存储时间大于3年（室温暗处储放）

1. 非接触式（sensor spot）O2传感器（见下左图）：用于非接触性测量监测透明容器中的氧气含量，传感器贴用硅胶等贴附在容器内壁，通过固定在外壁的光纤将荧光动态信号传输到测量仪以检测O2浓度；测量范围0-50%（0-23mg/l）（可选配其它范围），检测极限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精确度±0.2％（0.1mg/l）@20% O2，zui低使用寿命2千万数据点，存储时间大于3年（室温暗处储放）

1. 纳米微粒传感器（参见上右图）：纳米技术，用于非接触性测量微量液体中O2含量，即时响应，测量范围0-50%（0-23mg/l），检测极限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，存储时间大于3年（室温暗处储放）
2. 流通管：内置非接触式O2传感器，用于流动液体O2测量监测（如鱼类呼吸代谢测量等），测量范围0-50%（0-23mg/l）（可选配其它范围），检测极限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精确度±0.2％（0.1mg/l）@20% O2，zui低使用寿命1千万数据点，存储时间大于3年（室温暗处储放）
3. 呼吸瓶：内置非接触式O2传感器，用于生物呼吸测量（如藻类、小型鱼类、鱼卵、昆虫等），标准配置有4ml和20ml两种规格
4. Pyro Oxygen Logger软件用于参数设置、校准、数据显示包括图表显示、数据输出等功能

应用案例：

案例1：法国Bordeaux大学利用FSO2 4通道荧光光纤氧气测量仪，对Aquitaine海岸沉积样芯耗氧进行了测量分析，以研究海洋底栖动物活动（bioirrigation）对海岸带生态系统生态过程及生物地理化学功能（如沉积有机物的再矿化）的影响。

案例2：芬兰Turku大学利用FSO2和430μm光极氧探针，对南瓜类囊体悬浮液光合放氧进行了测量分析。

案例3：美国Woods Hole海洋学研究所，利用RF-O2非接触式光极氧气传感器（sensor spot），对海洋无脊椎动物呼吸代谢进行了测量分析，以研究其固有的生物钟与环境胁迫的关系，这些海洋无脊椎动物体重只有0.5－50mg。图中为翼足类软体动物在不同浓度CO2条件下的耗氧率。

案例4：澳大利亚海洋科学研究所、瑞典Gothenburg大学等组成的科学小组，利用Pyroscience的REDFLASH氧气测量技术，对河鲈（Perca fluviatilis）呼吸代谢进行测量分析，以研究其热耐受性和适应性的生理机制。他们选择波罗的海核电站附近的一个泻湖，核电站排出的热水进入该泻湖，在过去30年大量鱼类因为不适应水温升高而灭绝，但河鲈却得以繁盛，该地成为理想的研究气候变暖对鱼类种群影响的“天然实验室”。他们测量河鲈呼吸代谢率的同时，还测量其静脉血液在温度升高状态下的氧分压，静脉血是河鲈心脏供氧的主要来源，高温条件下静脉血氧气含量被认为是其心脏功能的重要限制因子。

案例5：德国Ulm大学利用FSO2测量仪和50μm可伸缩式RFO2探针，对患者脑脊髓液（CSF）样品溶解氧进行测量分析，以研究探讨神经紊乱及神经炎等疾病的生理和诊断。

案例6：德国农业科学与景观研究机构，利用FSO2测量仪和RFO2探针，对土壤氧气进行测量，以评估不同种类蚯蚓在低氧条件下对土壤改良的效率。

案例7：西班牙Valladolid大学利用RFO2荧光光纤氧气测量技术，监测葡萄酒橡木桶O2吸收——对葡萄酒品质至关重要但一直以来缺乏科学的了解。葡萄酒在橡木桶内（3－24个月）的过程溶解氧至关重要，因为O2调节了葡萄酒整个的熟化过程。

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2015

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2014

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17.      The effect of diel temperature and light cycles on the growth of Nannochloropsis oculata in a photobioreactor matrix. Tamburic et al., 2014, PLOS One, DOI: 10.1371/journal.pone.0086047

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22.      C*tion and isolation of N2-fixing bacteria from suboxic waters in the Baltic Sea?Bentzon-Tilia et al., 2014, FEMS Microbiol Ecol Vol 88 (2): 358-371

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24.      Temporary storage or permanent removal? The division of nitrogen between biotic assimilation and denitrification in stormwater biofiltration systems?Payne et al., 2014, PLOS One, DOI: 10.1371/journal.pone.0090890

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27.      Optimum temperatures for growth and feed conversion in cultured hapuku (Polyprion oxygeneios) – Is there a link to aerobic metabolic scope and final temperature preference??Khan et al., 2014, Aquaculture Vol 430: 107-113

28.      Aerobic scope does not predict the performance of a tropical eurythermal fish at elevated temperatures?Norin et al., 2014, J Exp Biol Vol 217: 244-251

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