Product Description

This high affinity anti-Flg (DYKDDDDK) antibody is developed using the Flg peptide (DYKDDDDK) conjugated to KLH and affinity-purified with the DYKDDDDK peptide. The purified antibody was conjugated to peroxidase (HRP) via reductive amination. The antibody is useful for detection, isolation and localization of DYKDDDDK-tag proteins.

Western blot analysis of the cell extract with Flg-tag protein expression cell lysate ( 1 mg/mL)

lane 2 = 25 µL; lane 3 = 15 µL; lane 4 = 10 µL cell lysate.

Western blot analysis of the cell extract with Flg-tag protein expression (lane 7 = 25 µg; lane 8 = 5 µg; lane 9 = 10 µg cell lysate)

Species

Rabbit

Formulation

250 µg/mL in PBS, 50% glycerol

Immunogen

Flg peptide (DYKDDDDK) KLH conjugates

Conjugation

Reductive amination; HRP/Ab ratio - 2:1

Specificity

The antibody recognizes DYKDDDDK-tag proteins.

Applications

ELISA (1:2500); WB (1 µg/mL)

Scientific Description

DYKDDDDK peptide (Flag-tag) is frequently used as an affinity tag to facilitate the detection, isolation, and localization of the DYKDDDDK-fusion proteins.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C. Expiration date is one year from date of shipping if stored properly.