Illumina 簇生成和测序试剂 MS-102-3003

Illumina公司，致力于新一代测序和芯片技术的生产与开发，提供产品与应用资讯

Illumina公司创立于1998年4月，是遗传变异和生物学功能分析领域的优秀的产品、技术和服务供应商。通过帮助客户加快实现生物信息的采集、分析和应用，来改善人类健康。

Sequencing Kits/ Microarray Kits/Informatics Products/ Clinical Research Products/In Vitro Diagnostic/ Products/Molecular Biology Reagents/ Accessory Products

上海易汇生物科技有限公司于17年正式销售Illumina公司基因芯片、二代测序NGS测序仪及相关测序试剂盒等产品

咨询illumina MiSeq测序试剂盒等产品欢迎您致电 上海易汇生物科技有限公司：1850 1609 238张经理

Illumina/TruSeq DNA Methylation Kit (12 reactions)/EGMK81312/1 Ea EGMK81312 Illumina 产品编号： EGMK81312美  元  价： $1009.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Unlock small samples (50–100 ng DNA input)

CpG, CHH, & CHG regions included for comprehensive, whole-genome results

Fast protocol – five-hour method

Capture full sample diversity

Sequence the entire sample–no loss of information

The process of bisulfite treatment denatures genomic DNA into single stranded DNA. TruSeq DNA Methylation converts single stranded DNA into an Illumina sequencing library. All ssDNA fragments are captured into an Illumina sequencing library, therefore eliminating the loss of diversity associated with other methods.

Supported analysis in the cloud

TruSeq DNA Methylation libraries can be aligned to the human genome and compared for differential methylation with Illumina BaseSpace Apps MethylSeq and MethylKit. These applications are fully supported and were developed specifically for TruSeq DNA Methylation library preparation.

Example data sets for TruSeq DNA Methylation libraries, also available in BaseSpace Sequence Hub Data Central (use the “Methyl Seq” category filter), demonstrate unparalleled quality and seamless analysis.

Deep coverage of critical genomic regions

Depth of coverage is enhanced in genomic areas with BIOLOGical utility. TruSeq DNA Methylation captures full sample diversity of critical areas, including:

Coding region start and end for exons from the canonical transcript of protein coding genes for genes known to be involved in cancer, taken from SOMA and CRUK panels (as well as literature-derived cancer genes)

Genes defined by the American College of Medical Genetics as being medically relevant (ACMG genes)

Exonic coding regions from Ensemble 70

List of 100 promoters defined by the Broad Institute as being of high interest and difficult to sequence"

"Illumina/TruSeq Small RNA Set C MiniSeq Kit (1 library prep and 2 MiniSeq reagents)/20005615/1 Ea

" 20005615 Illumina 产品编号： 20005615美  元  价： $3200.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:

View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)"

"Illumina/MiSeq FGx Reagent Kit/TG-143-1001/1 Ea

" TG-143-1001 Illumina 产品编号： TG-143-1001美  元  价： $1400.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

This sequencing kit for forensic genomics applications is based on the MiSeq Reagent Kit v3, which enables the highest output of all MiSeq kits. This kit allows forensic genomics laboratories to:

Simultaneously analyze a dense set of forensically relevant Markers at low, medium, or high sample throughput

Extend read lengths

Increase the number of reads to unlock new applications

The MiSeq FGx Reagent Kit uses pre-filled, ready-to-use reagent cartridges, and offers the improved v3 chemistry to increase cluster density and read length as well as improve quality.

MiSeq FGx Reagent Kit components are RFID-encoded and interact intelligently with the MiSeq FGx System to validate compatibility with forensic genomic applications.

Specifications:

Maximum Output 15 Gb

Maximum Reads per Run 14 million

Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis

Technology Sequencing

System Compatibility MiSeq FGx"

Illumina/TruSeq Targeted RNA Index Kit D (96 indexes, 384 samples)/RT-402-1004/1 Ea RT-402-1004 Illumina 产品编号： RT-402-1004美  元  价： $1019.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Targeted RNA Expression Kits enable highly customizable mid- to high-plex gene expression profiling studies.

You can define panels of 12-1,000 assays to target individual exons, isoforms, splice junctions, coding SNPS (cSNPs), gene fusions, and non-coding RNA transcripts, plus multiplex up to 384 samples. Generate the data equivalent of up to 25,000 individual RT-PCR assays in a single run.*

Assays include convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

Types of Study Designs

Custom panels—select up to 1000 targets from a database of over 400,000 predesigned assays.

Add-on (supplemental) content—add a second oligo pool of up to 1000 targets to a previously designed TruSeq Targeted RNA Expression custom panel.

Start a Custom Project

Order custom panels using DesignStudio, a free, user-friendly, web-based tool. Simply select the assays you need and add them to your order, with no design time.

Log in to DesignStudio

Browse TruSeq Targeted RNA Sample Data

Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using the TruSeq Targeted RNA Expression Kit.

These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.

Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

View TruSeq Targeted RNA Project Data

(secondary analysis with BaseSpace Apps)

*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Targeted RNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Transcript Variants

Specialized Sample Types FFPE

"

Illumina/TruSeq Small RNA Set B MiniSeq Kit (1 library prep and 2 MiniSeq reagents)/20005614/1 Ea 20005614 Illumina 产品编号： 20005614美  元  价： $3200.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:

View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~30 minutes

Input Quantity 25 to 100 ng total RNA from species with polyA tails

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex pooling with additional NeoPrep runs

Sample Throughput Up to 16 samples per NeoPrep library card

Species Category Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode

System Compatibility MiSeq,NextSeq 550,MiniSeq,NextSeq 500

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Variant Class Novel Transcripts,Transcript Variants

Method miRNA and Small RNA Sequencing"

Illumina/TruSeq Small RNA Library Prep Kit -Set B (24 rxns) (Set B: indexes 13-24)/RS-200-0024/1 Ea RS-200-0024 Illumina 产品编号： RS-200-0024美  元  价： $2450.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:

View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~30 minutes

Input Quantity 25 to 100 ng total RNA from species with polyA tails

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex pooling with additional NeoPrep runs

Sample Throughput Up to 16 samples per NeoPrep library card

Species Category Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode

System Compatibility MiSeq,NextSeq 550,MiniSeq,NextSeq 500

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Variant Class Novel Transcripts,Transcript Variants

Method miRNA and Small RNA Sequencing"

Illumina/TruSeq Stranded mRNA Library Prep Kit for NeoPrep (16 samples, 24 indexes)/NP-202-1001/1 Ea NP-202-1001 Illumina 产品编号： NP-202-1001美  元  价： $880.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Stranded mRNA Library Prep Kit for NeoPrep offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It enables robust interrogation of both standard and low-quality samples.

Simplified and seamless workflow solution – Generate libraries with minimal hands-on time

Unparalleled performance and reproducibility – Interrogate gene expression with TruSeq Stranded mRNA coverage and quality, and reduced user variABIlity

Low input requirement – Begin library prep with as little as 25 ng total RNA

Precise and Accurate

Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Cost-Efficient

Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.

NeoPrep System enhances TruSeq Stranded mRNA performance

The NeoPrep System enhances TruSeq Stranded mRNA performance by providing high-quality reproducIBLe results, even with low input amounts of RNA. Digital microfluidics technology precisely manipulates droplets that perform the library prep workflow, within the tightly controlled environment of the NeoPrep library card.

A simple, intuitive workflow delivers 16 libraries, eliminating most manual steps, and reducing hands-on time from ~4.5 hours to just 30 minutes. In addition, digital microfluidics requires less RNA input, enabling excellent performance from as little as 25 ng of total RNA.

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~30 minutes

Input Quantity 25 to 100 ng total RNA from species with polyA tails

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex pooling with additional NeoPrep runs

Sample Throughput Up to 16 samples per NeoPrep library card

System Compatibility Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000

Species Category Other,Mammalian,Bovine,Mouse,Human,Rat

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants

Technology Sequencing

Method mRNA Sequencing

Automation CapABIlity NeoPrep Digital Microfluidics"

Illumina/TruSeq Targeted RNA Index Kit A (96 indexes, 384 samples)/RT-402-1001/1 Ea RT-402-1001 Illumina 产品编号： RT-402-1001美  元  价： $1019.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Targeted RNA Expression Kits enable highly customizable mid- to high-plex gene expression profiling studies.

You can define panels of 12-1,000 assays to target individual exons, isoforms, splice junctions, coding SNPS (cSNPs), gene fusions, and non-coding RNA transcripts, plus multiplex up to 384 samples. Generate the data equivalent of up to 25,000 individual RT-PCR assays in a single run.*

Assays include convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

Types of Study Designs

Custom panels—select up to 1000 targets from a database of over 400,000 predesigned assays.

Add-on (supplemental) content—add a second oligo pool of up to 1000 targets to a previously designed TruSeq Targeted RNA Expression custom panel.

Start a Custom Project

Order custom panels using DesignStudio, a free, user-friendly, web-based tool. Simply select the assays you need and add them to your order, with no design time.

Log in to DesignStudio

Browse TruSeq Targeted RNA Sample Data

Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using the TruSeq Targeted RNA Expression Kit.

These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.

Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

View TruSeq Targeted RNA Project Data

(secondary analysis with BaseSpace Apps)

*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Targeted RNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Transcript Variants

Specialized Sample Types FFPE

"

Illumina/TruSeq Small RNA Library Prep Kit -Set C (24 rxns) (Set C: indexes 25-36)/RS-200-0036/1 Ea RS-200-0036 Illumina 产品编号： RS-200-0036美  元  价： $2450.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:

View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~30 minutes

Input Quantity 25 to 100 ng total RNA from species with polyA tails

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex pooling with additional NeoPrep runs

Sample Throughput Up to 16 samples per NeoPrep library card

Species Category Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode

System Compatibility MiSeq,NextSeq 550,MiniSeq,NextSeq 500

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Variant Class Novel Transcripts,Transcript Variants

Method miRNA and Small RNA Sequencing"

Illumina/TruSeq Small RNA Library Prep Kit -Set A (24 rxns) (Set A: indexes 1-12)/RS-200-0012/1 Ea RS-200-0012 Illumina 产品编号： RS-200-0012美  元  价： $2450.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:

View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~30 minutes

Input Quantity 25 to 100 ng total RNA from species with polyA tails

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex pooling with additional NeoPrep runs

Sample Throughput Up to 16 samples per NeoPrep library card

Species Category Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode

System Compatibility MiSeq,NextSeq 550,MiniSeq,NextSeq 500

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Variant Class Novel Transcripts,Transcript Variants

Method miRNA and Small RNA Sequencing"

Illumina/TruSeq Targeted RNA Index Kit C (96 indexes, 384 samples)/RT-402-1003/1 Ea RT-402-1003 Illumina 产品编号： RT-402-1003美  元  价： $1019.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Targeted RNA Expression Kits enable highly customizable mid- to high-plex gene expression profiling studies.

You can define panels of 12-1,000 assays to target individual exons, isoforms, splice junctions, coding SNPS (cSNPs), gene fusions, and non-coding RNA transcripts, plus multiplex up to 384 samples. Generate the data equivalent of up to 25,000 individual RT-PCR assays in a single run.*

Assays include convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

Types of Study Designs

Custom panels—select up to 1000 targets from a database of over 400,000 predesigned assays.

Add-on (supplemental) content—add a second oligo pool of up to 1000 targets to a previously designed TruSeq Targeted RNA Expression custom panel.

Start a Custom Project

Order custom panels using DesignStudio, a free, user-friendly, web-based tool. Simply select the assays you need and add them to your order, with no design time.

Log in to DesignStudio

Browse TruSeq Targeted RNA Sample Data

Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using the TruSeq Targeted RNA Expression Kit.

These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.

Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

View TruSeq Targeted RNA Project Data

(secondary analysis with BaseSpace Apps)

*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Targeted RNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Transcript Variants

Specialized Sample Types FFPE

"

Illumina/TruSeq DNA Methylation Kit (96 reactions)/EGMK91396/1 Ea EGMK91396 Illumina 产品编号： EGMK91396美  元  价： $5567.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Unlock small samples (50–100 ng DNA input)

CpG, CHH, & CHG regions included for comprehensive, whole-genome results

Fast protocol – five-hour method

Capture full sample diversity

Sequence the entire sample–no loss of information

The process of bisulfite treatment denatures genomic DNA into single stranded DNA. TruSeq DNA Methylation converts single stranded DNA into an Illumina sequencing library. All ssDNA fragments are captured into an Illumina sequencing library, therefore eliminating the loss of diversity associated with other methods.

Supported analysis in the cloud

TruSeq DNA Methylation libraries can be aligned to the human genome and compared for differential methylation with Illumina BaseSpace Apps MethylSeq and MethylKit. These applications are fully supported and were developed specifically for TruSeq DNA Methylation library preparation.

Example data sets for TruSeq DNA Methylation libraries, also available in BaseSpace Sequence Hub Data Central (use the “Methyl Seq” category filter), demonstrate unparalleled quality and seamless analysis.

Deep coverage of critical genomic regions

Depth of coverage is enhanced in genomic areas with BIOLOGical utility. TruSeq DNA Methylation captures full sample diversity of critical areas, including:

Coding region start and end for exons from the canonical transcript of protein coding genes for genes known to be involved in cancer, taken from SOMA and CRUK panels (as well as literature-derived cancer genes)

Genes defined by the American College of Medical Genetics as being medically relevant (ACMG genes)

Exonic coding regions from Ensemble 70

List of 100 promoters defined by the Broad Institute as being of high interest and difficult to sequence"

Illumina/TruSeq Targeted RNA Index Kit (48 indexes, 48 samples)/RT-401-1001/1 Ea RT-401-1001 Illumina 产品编号： RT-401-1001美  元  价： $698.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Targeted RNA Expression Kits enable highly customizable mid- to high-plex gene expression profiling studies.

You can define panels of 12-1,000 assays to target individual exons, isoforms, splice junctions, coding SNPS (cSNPs), gene fusions, and non-coding RNA transcripts, plus multiplex up to 384 samples. Generate the data equivalent of up to 25,000 individual RT-PCR assays in a single run.*

Assays include convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

Types of Study Designs

Custom panels—select up to 1000 targets from a database of over 400,000 predesigned assays.

Add-on (supplemental) content—add a second oligo pool of up to 1000 targets to a previously designed TruSeq Targeted RNA Expression custom panel.

Start a Custom Project

Order custom panels using DesignStudio, a free, user-friendly, web-based tool. Simply select the assays you need and add them to your order, with no design time.

Log in to DesignStudio

Browse TruSeq Targeted RNA Sample Data

Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using the TruSeq Targeted RNA Expression Kit.

These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.

Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

View TruSeq Targeted RNA Project Data

(secondary analysis with BaseSpace Apps)

*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Targeted RNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Transcript Variants

Specialized Sample Types FFPE

"

"Illumina/TruSeq DNA Methylation Kit (24 reactions)/EGMK91324/1 Ea

" EGMK91324 Illumina 产品编号： EGMK91324美  元  价： $1787.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Unlock small samples (50–100 ng DNA input)

CpG, CHH, & CHG regions included for comprehensive, whole-genome results

Fast protocol – five-hour method

Capture full sample diversity

Sequence the entire sample–no loss of information

The process of bisulfite treatment denatures genomic DNA into single stranded DNA. TruSeq DNA Methylation converts single stranded DNA into an Illumina sequencing library. All ssDNA fragments are captured into an Illumina sequencing library, therefore eliminating the loss of diversity associated with other methods.

Supported analysis in the cloud

TruSeq DNA Methylation libraries can be aligned to the human genome and compared for differential methylation with Illumina BaseSpace Apps MethylSeq and MethylKit. These applications are fully supported and were developed specifically for TruSeq DNA Methylation library preparation.

Example data sets for TruSeq DNA Methylation libraries, also available in BaseSpace Sequence Hub Data Central (use the “Methyl Seq” category filter), demonstrate unparalleled quality and seamless analysis.

Deep coverage of critical genomic regions

Depth of coverage is enhanced in genomic areas with BIOLOGical utility. TruSeq DNA Methylation captures full sample diversity of critical areas, including:

Coding region start and end for exons from the canonical transcript of protein coding genes for genes known to be involved in cancer, taken from SOMA and CRUK panels (as well as literature-derived cancer genes)

Genes defined by the American College of Medical Genetics as being medically relevant (ACMG genes)

Exonic coding regions from Ensemble 70

List of 100 promoters defined by the Broad Institute as being of high interest and difficult to sequence"

"Illumina/TruSeq Small RNA Set D MiniSeq Kit (1 library prep and 2 MiniSeq reagents)/20005616/1 Ea

" 20005616 Illumina 产品编号： 20005616美  元  价： $3200.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:

View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~30 minutes

Input Quantity 25 to 100 ng total RNA from species with polyA tails

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex pooling with additional NeoPrep runs

Sample Throughput Up to 16 samples per NeoPrep library card

Species Category Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode

System Compatibility MiSeq,NextSeq 550,MiniSeq,NextSeq 500

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Variant Class Novel Transcripts,Transcript Variants

Method miRNA and Small RNA Sequencing"

Illumina/TruSeq DNA Methylation Index PCR Primers (10 reactions, 12 indexes)/EGIDX81312/1 Ea EGIDX81312 Illumina 产品编号： EGIDX81312美  元  价： $78.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Unlock small samples (50–100 ng DNA input)

CpG, CHH, & CHG regions included for comprehensive, whole-genome results

Fast protocol – five-hour method

Capture full sample diversity

Sequence the entire sample–no loss of information

The process of bisulfite treatment denatures genomic DNA into single stranded DNA. TruSeq DNA Methylation converts single stranded DNA into an Illumina sequencing library. All ssDNA fragments are captured into an Illumina sequencing library, therefore eliminating the loss of diversity associated with other methods.

Supported analysis in the cloud

TruSeq DNA Methylation libraries can be aligned to the human genome and compared for differential methylation with Illumina BaseSpace Apps MethylSeq and MethylKit. These applications are fully supported and were developed specifically for TruSeq DNA Methylation library preparation.

Example data sets for TruSeq DNA Methylation libraries, also available in BaseSpace Sequence Hub Data Central (use the “Methyl Seq” category filter), demonstrate unparalleled quality and seamless analysis.

Deep coverage of critical genomic regions

Depth of coverage is enhanced in genomic areas with BIOLOGical utility. TruSeq DNA Methylation captures full sample diversity of critical areas, including:

Coding region start and end for exons from the canonical transcript of protein coding genes for genes known to be involved in cancer, taken from SOMA and CRUK panels (as well as literature-derived cancer genes)

Genes defined by the American College of Medical Genetics as being medically relevant (ACMG genes)

Exonic coding regions from Ensemble 70

List of 100 promoters defined by the Broad Institute as being of high interest and difficult to sequence"

Illumina/TruSeq Bovine Parentage Kit (96 indexes, 96 samples)/20004795/1 Ea 20004795 Illumina 产品编号： 20004795美  元  价： $960.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

This solution for parentage testing of multiple beef and dairy cattle breeds has added genotyping of relevant traits. By offering more information in a single assay than from parentage testing alone, service labs can use the TruSeq Bovine Parentage Sequencing Panel to make more informed herd management recommendations.

Includes all International Society of Animal Genetics (ISAG) recommended content, plus disease-associated and economically relevant traits

Content design, high accuracy, and reliABIlity eliminate need for multiple or repeat testing

Analysis software converts sequencing results to genotype calls without the need for bioinformatics expertise

The TruSeq Bovine Parentage Sequencing Panel uses proven Illumina next-generation sequencing (NGS) technology to deliver accurate genotyping results for SNP-based parentage determination in cattle. The high accuracy and reliABIlity of Illumina sequencing reduces the amount of required testing and shortens the time to answer, leADIng to efficiency gains and cost savings. It features a streamlined workflow that goes from DNA to report in 1.5 days with minimal hands-on time.

Have confidence that the typing result is accurate, the first time. TruSeq Bovine Parentage has been tested with multiple breeds, DNA inputs, and extraction methods. It provides deep sequencing coverage across SNPs.

Specifications:

Input Quantity ≤ 1 ng genomic DNA, or 2 ul crude lysates, or 1.2 mm FTA card punch

Hands-On Time ~1 hour

Assay Time 9 hours

Mechanism of Action PCR

Multiplexing Maximum of 32 casework samples or 96 database or reference samples

System Compatibility MiSeq

Variant Class Single Nucleotide Polymorphisms (SNPs)

Species Category Bovine

Technology Sequencing

Method Genotyping by Sequencing,Amplicon Sequencing,Targeted DNA Sequencing"

"Illumina/TruSeq Ribo Profile for Mammalian (12 reactions, 12 indexes)/RPHMR12126/1 Ea

" RPHMR12126 Illumina 产品编号： RPHMR12126美  元  价： $1575.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Study active mRNA translation with sequencing

Sequence ribosome-protected mRNA

Predict protein abundance

Investigate translational control

Measure gene expression

Rapid, scalable spin-column method

No ultracentrifuge required

CompatIBLe with yeast and mammalian samples

Sequence actively translated transcripts

Ribosome profiling is a technique for investigating translational control that provides a snapshot of all the ribosomes active in a cell at a specific time. TruSeq Ribo Profile generates RNA-Seq libraries from ribosome-protected fragments of mRNAs that are actively being translated.

Use TruSeq Ribo Profile to sequence mRNA fragments undergoing translation by ribosomes. These mRNA fragments are called ""footprinted"" or ribosome-protected mRNA fragments.

You can also identify proteins being actively translated from samples prepared with TruSeq Ribo Profile. Samples collected at different times often show changes in translation. Samples treated with different drugs often show different translation patterns.

Specifications:

Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500

Automation CapABIlity Liquid Handling Robots

Variant Class Transcript Variants

Species Category Rat"

Illumina/TruSeq Small RNA Library Prep Kit -Set D (24 rxns) (Set D: indices 37-48)/RS-200-0048/1 Ea RS-200-0048 Illumina 产品编号： RS-200-0048美  元  价： $2450.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:

View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~30 minutes

Input Quantity 25 to 100 ng total RNA from species with polyA tails

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex pooling with additional NeoPrep runs

Sample Throughput Up to 16 samples per NeoPrep library card

Species Category Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode

System Compatibility MiSeq,NextSeq 550,MiniSeq,NextSeq 500

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Variant Class Novel Transcripts,Transcript Variants

Method miRNA and Small RNA Sequencing"

Illumina/TruSeq Ribo Profile for Yeast (12 reactions, 12 indexes)/RPYSC12116/1 Ea RPYSC12116 Illumina 产品编号： RPYSC12116美  元  价： $1575.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Study active mRNA translation with sequencing

Sequence ribosome-protected mRNA

Predict protein abundance

Investigate translational control

Measure gene expression

Rapid, scalable spin-column method

No ultracentrifuge required

CompatIBLe with yeast and mammalian samples

Sequence actively translated transcripts

Ribosome profiling is a technique for investigating translational control that provides a snapshot of all the ribosomes active in a cell at a specific time. TruSeq Ribo Profile generates RNA-Seq libraries from ribosome-protected fragments of mRNAs that are actively being translated.

Use TruSeq Ribo Profile to sequence mRNA fragments undergoing translation by ribosomes. These mRNA fragments are called ""footprinted"" or ribosome-protected mRNA fragments.

You can also identify proteins being actively translated from samples prepared with TruSeq Ribo Profile. Samples collected at different times often show changes in translation. Samples treated with different drugs often show different translation patterns.

Specifications:

Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500

Automation CapABIlity Liquid Handling Robots

Variant Class Transcript Variants

Species Category Rat"

Illumina/TruSeq Targeted RNA Index Kit B (96 indexes, 384 samples)/RT-402-1002/1 Ea RT-402-1002 Illumina 产品编号： RT-402-1002美  元  价： $1019.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Targeted RNA Expression Kits enable highly customizable mid- to high-plex gene expression profiling studies.

You can define panels of 12-1,000 assays to target individual exons, isoforms, splice junctions, coding SNPS (cSNPs), gene fusions, and non-coding RNA transcripts, plus multiplex up to 384 samples. Generate the data equivalent of up to 25,000 individual RT-PCR assays in a single run.*

Assays include convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

Types of Study Designs

Custom panels—select up to 1000 targets from a database of over 400,000 predesigned assays.

Add-on (supplemental) content—add a second oligo pool of up to 1000 targets to a previously designed TruSeq Targeted RNA Expression custom panel.

Start a Custom Project

Order custom panels using DesignStudio, a free, user-friendly, web-based tool. Simply select the assays you need and add them to your order, with no design time.

Log in to DesignStudio

Browse TruSeq Targeted RNA Sample Data

Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using the TruSeq Targeted RNA Expression Kit.

These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.

Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

View TruSeq Targeted RNA Project Data

(secondary analysis with BaseSpace Apps)

*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Targeted RNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Transcript Variants

Specialized Sample Types FFPE

"

Illumina/HiSeq Rapid Duo cBot Sample Loading Kit/CT-403-2001/1 Ea CT-403-2001 Illumina 产品编号： CT-403-2001美  元  价： $440.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

The HiSeq Rapid Duo cBot Sample LoADIng Kit allows maximum indexing freedom in rapid run mode by enabling individually addressable lanes. This kit is an optional accessory for HiSeq Rapid Cluster kits (which require a cBot instrument), and is designed for use in the rapid run workflow of HiSeq 2500 and 1500 sequencers.

Load each flow cell lane independently

Maximize indexing possibilities

Separate precious samples physically

Load different samples in each lane

The HiSeq Rapid Duo cBot Sample LoADIng Kit allows each rapid flow cell lane to be loaded with a unique sample template pool. The duo kit accomplishes individual lane loADIng by allowing a cBot instrument to be used for the template hybridization and first extension steps of the cluster generation process. To use the cBot to load 2 libraries onto a rapid flow cell, 1 HiSeq Rapid Duo cBot Sample LoADIng Kit is required. Each kit contains sufficient reagents for loADIng 1 flow cell.

A specifically designed duo manifold directs each of the 2 samples into the 2 separate rapid flow cell lanes. The cBot portion takes about an hour, after which the flow cell is moved to the HiSeq 2500 or 1500 for further cluster generation steps using a HiSeq Rapid Cluster Kit."

Illumina/PhiX Control v3/FC-110-3001/1 Ea FC-110-3001 Illumina "产品编号： FC-110-3001美  元  价： $160.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents

Size: 1 Ea" "Product Highlights:

PhiX Control v3 is a reliable, adapter-ligated library used as a control for Illumina sequencing runs. The library is derived from the small, well-characterized PhiX genome, offering several benefits for sequencing and alignment.

The versatile PhiX Control v3 is provided as a ready-to-use library, and can be utilized in diverse applications to add value to your workflow and increase confidence in your results.

The PhiX library provides a quality control for cluster generation, sequencing, and alignment, and a calibration control for cross-talk matrix generation, phasing, and prephasing. It can be rapidly aligned to estimate relevant sequencing by synthesis (SBS) metrics such as phasing and error rate.

Depending on the application, PhiX Control v3 may also be utilized:

As a high-concentration spike-in control for unbalanced samples (genomes with AT or GC content of less than 40% or greater than 60%)

As a low-concentration spike-in control for alignment calculations and quantification efficiency

As a dedicated control lane alongside low-diversity samples

As a control for troubleshooting cluster generation problems, to help determine whether an error is related to library preparation

Specifications:

System Compatibility MiSeq,Genome Analyzer IIx,HiSeq 2000,HiSeq X Ten,HiScanSQ,NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Reagent Type Sequencing Control

Technology Sequencing"

Illumina/TruSeq Small RNA Set A MiniSeq Kit (1 library prep and 2 MiniSeq reagents)/20005613/1 Ea 20005613 Illumina 产品编号： 20005613美  元  价： $3200.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:

View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~30 minutes

Input Quantity 25 to 100 ng total RNA from species with polyA tails

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex pooling with additional NeoPrep runs

Sample Throughput Up to 16 samples per NeoPrep library card

Species Category Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode

System Compatibility MiSeq,NextSeq 550,MiniSeq,NextSeq 500

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Variant Class Novel Transcripts,Transcript Variants

Method miRNA and Small RNA Sequencing"

Illumina

Illumina/Infinium Accessory Starter Kit - Mid Throughput (110V)/WG-15-306/1 Ea WG-15-306 Illumina 产品编号： WG-15-306美  元  价： $78000.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The Infinium iSelect offers the ABIlity to interrogate virtually any target (e.g. SNP, CNV, INDEL) across any species. Take advantage of the proven Infinium assay technology to create a fully-customized design that is tailored to your unique study needs.

Design a custom genotyping panel that supports up to 700k custom targets (SNPs, indels, and CNVs). The Infinium iSelect can be deployed on either the 24-sample HD (3072 to 90,000 BeadTypes) or 24-sample HTS (90,001 to 700,000 BeadTypes). Creation of these custom assays enables focused, high-throughput genotyping applications tailored to specific project needs.

Keep up-to-date with new discoveries by revising custom content through the Infinium iSelect + (add-on content) option. The Infinium iSelect+ option allows researchers to combine existing Marker sets with new, unique content to create a single BeadChip, thereby increasing the efficiency and cost-effectiveness of custom studies.

Begin custom design and order process

Specifications:

Number of Markers iSelect HD: 3072 to 90,000 custom Markers,iSelect HTS: 90,000 to 700,000 custom Markers

Sample Throughput Up to 5760 samples per week

Input Quantity 200 ng

Species Category Any Species

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium Multi-Ethnic AMR/AFR-8 v1.0 Kit (48 samples)/20001091/1 Ea

" 20001091 Illumina 产品编号： 20001091美  元  价： $5232.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Infinium Multi-Ethnic AMR "Product Highlights:

The Infinium Multi-Ethnic AMR/AFR-8 Kit provides a multi-purpose, multi-ethnic genotyping array focused on Hispanic and African American populations. With > 1.4 million expertly selected Markers, it enables identification of genetic associations with common and rare traits, providing insight to epidemiologists, health care researchers, population geneticists, and genomic researchers.

Expert-selected content

This kit combines expertly selected Markers and content from the most popular Illumina commercial arrays with the most current genomic information. Researchers can detect both common and rare variants across Hispanic and African American populations and impute variants in a vast number of subpopulations.

Maximized imputation accuracy

Consortium partners developed content for this multi-ethnic AMR/AFR genotyping array using tagging strategies with the power to perform more effective association studies in Hispanic and African American populations. The novel algorithm selects population-specific and transethnic tag SNPs that maximize imputation accuracy, as imputation has become a standard practice in the interpretation of genotyping data and allows for more accurate statistical inference of genotypes not directly genotyped.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 1,430,141u003cbru003eCustom Marker add-on capacity: Up to 245,000

Sample Throughput ~1067 samples per week

Input Quantity 200 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/BovineLD v2.0 BeadChip (48 samples)/WG-451-2001/1 Ea

" WG-451-2001 Illumina 产品编号： WG-451-2001美  元  价： $1200.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The BovineLD BeadChip microarray kit enables accurate genotyping to understand the impact of genetics on milk production, reproduction, health, and more. Delivering superior, scalable content at an economical price, it allows you to extend genomic selection to the entire herd. This array offers:

A robust imputation tool to estimate genomic breeding value accurately

High call rates, with demonstrated imputation efficiency of > 98%

Expertly selected content that can be enhanced with up to 80,000 custom Markers

Contains all 200 SNPs on the ISAG panel for bovine parentage (100 core SNPs and 100 additional SNPs)

The ABIlity to interrogate up to 24 samples in parallel

Cost-Effective Genotyping of Herd Animals

The BovineLD v2.0 BeadChip, together with the Infinium BovineSNP50, Infinium BovineHD, and iSelect Custom BeadChips, creates a broad genotyping portfolio that breeders can rely on to characterize genetic variation and accurately estimate genomic breeding value.

While the BovineHD and BovineSNP50 BeadChips provide superior power to interrogate genetic variation in high-value animals and support genome-wide studies, the BovineLD v2.0 BeadChip enables the cost-effective genotyping of lower-value animals.

High Imputation Accuracy

The BeadChip’s strategically selected SNPs possess demonstrated reliABIlity, high average minor allele frequency (MAF), uniform distribution across the bovine genome, and excellent imputation performance for a range of global dairy breeds. The 7,931 SNPs on the BovineLD v2.0 BeadChip were subjected to rigorous functional testing on multiple breeds to ensure strong performance. Illumina ensures that every BovineLD BeadChip offers > 99% average call rate across common dairy and beef cattle breeds.

Superior, Scalable Content

Illumina developed the BovineLD v2.0 BeadChip as part of a BovineLD Consortium in collaboration with global bovine agricultural thought leaders. Illumina scientists and collaborators referenced historical data generated with the BovineSNP50 BeadChip to identify the best SNP content for imputation efficiency among global dairy breeds.1

In silico testing determined that the highest imputation efficiency could be achieved by optimizing MAF among targeted breeds and evenly spacing SNPs across the entire bovine genome with higher Marker densities at the chromosomal ends. Content includes coverage of all chromosomes, including X, known Y haplotypes, and mitochondrial DNA.

High-Throughput Format

The Infinium Assay powers this multi-sample genotyping panel, delivering the industry’s highest call rates and reproducibility. The assay’s PCR-free single-tube sample preparation significantly reduces labor and potential sample handling errors.2,3 A multi-sample format further reduces experimental variABIlity and overall project cost by allowing breeders to interrogate up to 24 samples in parallel.

Learn More About Plant and Animal Genotyping

Specifications:

Input Quantity 200 ng DNA (at 50 ng/ul)

Number of Markers Fixed Markers: 7931 evenly spaced SNPs across the genomeu003cbru003eCustom Marker add-on capacity: Up to 80,000 (with + kit versions)

Technology Microarray

Species Category Bovine

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium® XT Upgrade Kit (24 Beadchips)/20011101/1 Ea

" 20011101 Illumina 产品编号： 20011101美  元  价： $27531.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

Comprehensive Production-Scale Screening Solution

This solution enables large-scale genetic improvement programs in agrigenomics and supports large-scale screening for biobanks and personalized medicine initiatives. It uses a high-efficiency, flexIBLe workflow.

Production Scale: Optimized assay and 96-sample BeadChip enables processing of 100,000 to upwards of 1,000,000 samples/year per lab

FlexIBLe Content: Targeted single or multispecies assays of 100s to 50,000 SNPs with at least 95% conversion rate guaranteed for custom panels

High Efficiency Workflow: Total assay turnaround time reduced from 3 days to 2 days with less hands-on time

Infinium XT simplifies the custom assay design process, reduces overall hands-on time, and enhances automation robot performance and utilization. The workflow offers an option to reduce the overall turnaround time, if the time to answer is more important than maximum weekly output.

Streamlined Sample Preparation and Data Analysis

A high-throughput solution like Infinium XT needs integrated systems that streamline sample preparation and analysis. The Illumina Automation Control software for the Tecan liquid handling robot has been updated to increase sample processing efficiency. An enhanced data analysis software solution offers real-time data generation and on-demand QC report functionality so production issues can be identified and corrected earlier in the process.

Updated software for GenomeStudio speeds up genotype cluster generation, reducing overall analysis turnaround time. Also, a genotyping module for agricultural and other applications involving polyploid species is now included.

After a cluster file is created, production-level analysis can be transitioned to Beeline Software. This software significantly reduces the time needed to observe quality performance specifications, generate genotyping reports, and analyze polyploid data, without removing the flexibility for manual intervention.

Specifications:

Sample Throughput 100,000 to u003e 1,000,000 samples/year

Number of Markers 100s to 50,000 Markers of interest

Species Category Any Species

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Insertions-Deletions (indels)

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan

"

"Illumina/Infinium® Omni5Exome-4 v1.3 Kit (16 samples)/20005140/1 Ea

" 20005140 Illumina 产品编号： 20005140美  元  价： $4448.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

Infinium Omni5Exome-4 BeadChip delivers comprehensive coverage of the genome, providing functional exonic content for whole-genome genotyping and copy number variation (CNV) analysis. The BeadChips include optimized tag SNPs targeting genetic variation down to 1% minor allele frequency (MAF).

Using the proven iScan array scanner and integrated analysis software, these BeadChips offer optimized tag SNPs, exonic content, and fully supported CNV analysis. Combined with convenient packaging and a streamlined PCR-free protocol, these kits provide a comprehensive DNA analysis solution.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 4,548,474

Sample Throughput ~460 samples per week

Input Quantity 400 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium ImmunoArray-24 v2 BeadChip Kit (1152 samples)/WG-357-1003/1 Ea

" WG-357-1003 Illumina 产品编号： WG-357-1003美  元  价： $49536.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The Infinium ImmunoArray-24 v2 BeadChip is the next-generation genotyping array for detecting genetic variation in the human immune system. This BeadChip includes the most valuable content from the HumanImmuno v1 BeadChip, supplemented with newly selected content produced by the immunology research community. This new content includes the majority of the loci currently published in the GWAS catalog for major autoimmune and inflammatory disorders1 as well as published ancestry informative Markers.

Valuable Content

Infinium ImmunoArray-24 v2 BeadChip content highlights:

Over 250,000 immune specific Markers

Over 85,000 new Markers

Includes most valuable content from HumanImmuno v1 BeadChip

Focused on 17 major autoimmune disorders

FlexIBLe Format

The Infinium ImmunoArray-24 v2 can be tailored to incorporate up to ~390,000 custom beadtypes. This BeadChip is available as a standalone version as well as an add-on to several standard Illumina genotyping BeadChips.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: ~253,702,Custom Marker add-on capacity: Up to 390,000 (with + kit versions)

Sample Throughput ~5760 samples per week

Input Quantity 200 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Method Targeted Genotyping Array

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium OmniZhongHua-8 v1.3 Kit (96 samples)/20004339/1 Ea

" 20004339 Illumina 产品编号： 20004339美  元  价： $10560.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The Infinium OmniZhongHua-8 BeadChip delivers exceptional coverage of common, intermediate, and rare variation found within Chinese populations for genome-wide association studies (GWAS). Optimized tag SNP content from all three HapMap phases and the 1000 Genomes Project (1kGP) has been strategically selected to create a population-focused array for the discovery of novel disease and trait associations in Chinese populations.

The Infinium OmniZhongHua-8 BeadChip allows profiling of > 890,000 Markers per sample. The assay is deployed with proprietary BeadArray technology, delivering exceptionally high data quality with regards to call rates (average > 99%), reproducibility (> 99.9%), and low sample repeat rates. High signal-to-noise ratios and low overall noise levels allow for precise, reliable calls and copy number analyses.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 878,291,Custom Marker add-on capacity: None

Sample Throughput ~960 samples per week

Input Quantity 200 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium® Omni5Exome-4 v1.3 Kit (48 samples)/20005141/1 Ea

" 20005141 Illumina 产品编号： 20005141美  元  价： $13344.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

Infinium Omni5Exome-4 BeadChip delivers comprehensive coverage of the genome, providing functional exonic content for whole-genome genotyping and copy number variation (CNV) analysis. The BeadChips include optimized tag SNPs targeting genetic variation down to 1% minor allele frequency (MAF).

Using the proven iScan array scanner and integrated analysis software, these BeadChips offer optimized tag SNPs, exonic content, and fully supported CNV analysis. Combined with convenient packaging and a streamlined PCR-free protocol, these kits provide a comprehensive DNA analysis solution.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 4,548,474

Sample Throughput ~460 samples per week

Input Quantity 400 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium® Omni5-4 v1.2 Kit (384 samples)/20005153/1 Ea

" 20005153 Illumina 产品编号： 20005153美  元  价： $104832.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The Infinium Omni5-4 BeadChip delivers comprehensive coverage of the genome, leveraging powerful tagSNPs selected from the International HapMap and 1000 Genomes Projects that target genetic variation down to 1% minor allele frequency (MAF).

Using the proven iScan System, this four-sample BeadChip offers high-throughput sample processing, and optimized content for whole-genome genotyping and CNV applications.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 4,284,426

Sample Throughput u003e460 samples per week

Input Quantity 400 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium Multi-Ethnic EUR/EAS/SAS-8 v1.0 Kit (96 samples)/20001102/1 Ea

" 20001102 Illumina 产品编号： 20001102美  元  价： $10464.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Infinium Multi-Ethnic EUR "Product Highlights:

The Infinium Multi-Ethnic EUR/EAS/SAS-8 Kit provides a multi-purpose, multi-ethnic genotyping array focused on European, East Asian, and South Asian populations. With > 1.4 million expertly selected Markers, it enables identification of genetic associations with common and rare traits, providing insight to epidemiologists, health care researchers, population geneticists, and genomic researchers.

Expert-selected content

This kit combines expertly selected Markers and content from the most popular Illumina commercial arrays with the most current genomic information. Researchers can detect both common and rare variants across European, East Asian, and South Asian populations and impute variants in a vast number of subpopulations.

Maximized imputation accuracy

Consortium partners developed content for this multi-ethnic EUR/EAS/SAS genotyping array using tagging strategies with the power to perform more effective association studies in European, East Asian, and South Asian populations. The novel algorithm selects population-specific and transethnic tag SNPs that maximize imputation accuracy, as imputation has become a standard practice in the interpretation of genotyping data and allows for more accurate statistical inference of genotypes not directly genotyped.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 1,475,140u003cbru003eCustom Marker add-on capacity: Up to 245,000

Sample Throughput ~1067 samples per week

Input Quantity 200 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium Exome-24 v1.1 Kit (48 samples)/20015246/1 Ea

" 20015246 Illumina 产品编号： 20015246美  元  价： $2256.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

Infinium Exome-24 BeadChip delivers exceptional coverage of putative functional exonic variants selected from over 12,000 individual exome and whole-genome sequences. Markers were identified through a close collaboration with leADIng geneticists with the goal of developing an extensive catalog of exome variants.

The exonic content consists of >240,000 Markers representing diverse populations - including European, African, Chinese, and Hispanic individuals - and a range of common conditions, such as type 2 diabetes, cancer, metabolic, and psychiatric disorders.

Infinium Exome-24 BeadChip delivers focused coverage of exonic regions, but does not include coverage outside of coding regions. For greater flexibility, the Infinium Exome-24+ version of the BeadChip can be customized to include up to 400,000 additional Markers.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 244,883,Custom Marker add-on capacity: Up to 400,000 (with + kit versions)

Sample Throughput ~2304 samples per week

Input Quantity 200 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/MaizeSNP50 BeadChip (288 samples)/WG-500-1002/1 Ea

" WG-500-1002 Illumina 产品编号： WG-500-1002美  元  价： $14400.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The MaizeSNP50 DNA Analysis Kit enables the interrogation of genetic variation across maize lines. Illumina developed this BeadChip array in collaboration with TraitGenetics, the French National Institute for Agricultural Research (INRA), and Syngenta, with the SNP content selected from several public and private sources. This BeadChip contains more than 50,000 validated Markers derived from the B73 reference sequence.

The highly polymorphic SNP content on the MaizeSNP50 BeadChip was subjected to rigorous functional testing across over 30 diverse maize lines to ensure strong performance. Importantly, this BeadChip presents an average of greater than 25 Markers per megabase (Mb), providing ample SNP density for robust whole-genome genotyping studies. In addition, the MaizeSNP50 Marker set increases the ABIlity to perform corn genetic mapping and Marker assisted breeding.

The MaizeSNP50 BeadChip provides high call rates and allows for flexIBLe content deployment.

The assay's single-tube sample preparation without PCR or ligation steps significantly reduces labor and potential sample handling errors

The BeadChip further reduces experimental variABIlity by allowing researchers to interrogate up to 24 samples in parallel

For a low-density option, see the MaizeLD BeadChip Kit.

Learn More About Plant and Animal Genotyping

Specifications:

Input Quantity 200 ng at 50 ng/µl

Number of Markers Fixed Markers: 56,110 evenly distributed SNPs across the genomeu003cbru003eCustom Marker add-on capacity: None

Method Genome-Wide Genotyping Array

Technology Microarray

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan

Species Category Maize"

"Illumina/Infinium CoreExome-24 v1.2 Kit (1152 samples)/20015264/1 Ea

" 20015264 Illumina 产品编号： 20015264美  元  价： $62208.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The customizable Infinium CoreExome-24 BeadChip offers an economical way to perform and support large genetic studies, especially large-scale genotyping studies. They can also be used to quickly and easily obtain baseline sample data sets for a variety of downstream applications, including common variant, sex confirmation, mtDNA, ancestry, loss-of-variant, indel, and CNV studies.

Developed in collaboration with several leADIng research institutions, the Infinium CoreExome-24 BeadChip includes all the tag SNPs found on the Infinium Core-24 BeadChip, plus over 240,000 Markers from the Infinium Exome-24 BeadChip. For greater flexibility, the Infinium CoreExome-24+ version of the BeadChip can be customized to include up to 100,000 additional Markers.

The Infinium CoreExome-24 BeadChips use the Infinium high-throughput screening (HTS) 24-sample format, enabling high content flexibility, throughput capacity, and genotyping accuracy. When combined with the proven iScan System, these BeadChips deliver affordable, high-quality, genome-wide information across diverse world populations.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 550,601,Custom Marker add-on capacity: Up to 100,000 (with + kit versions)

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium Core-24 Kit v1.1 (48 samples)/20015254/1 Ea

" 20015254 Illumina 产品编号： 20015254美  元  价： $2352.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The customizable Infinium Core-24 BeadChip offers an economical way to perform and support large genetic studies. Developed in collaboration with several leADIng research institutions, the Infinium Core-24 BeadChip contains highly-informative genome-wide tag SNPs found across diverse world populations, additional high-value Markers (including indels and updated exome-focused content), and has capacity to include up to 300,000 semi-custom Markers.

In addition to cost-effectively performing large-scale genotyping studies, this BeadChip can be used to quickly and easily obtain baseline sample datasets for a variety of downstream applications, including common variant, mtDNA, ancestry, sex confirmation, loss-of-variant, indel, and CNV detection studies.

The Infinium Core-24 BeadChip is based upon the trusted Infinium assay. Using the proven iScan System, this 24-sample BeadChip combines affordABIlity with high-throughput sample processing to deliver high-quality, genome-wide information.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 307,342,Custom Marker add-on capacity: Up to 300,000 (with + kit versions)

Sample Throughput 2304 samples per week

Input Quantity 200 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/CanineHD BeadChip (1152 samples)/WG-440-1003/1 Ea

" WG-440-1003 Illumina 产品编号： WG-440-1003美  元  价： $97920.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

Featuring highly polymorphic single-nucleotide polymorphism (SNP) content and providing uniform genomic coverage, the CanineHD BeadChip array enables the interrogation of genetic variation in any domestic dog breed.

Importantly, this BeadChip presents an average of greater than 70 Markers per megabase (Mb), providing ample SNP density for robust within-breed association and copy number variation (CNV) studies.

This BeadChip contains more than 170,000 Markers placed on the CanFam2.0 reference sequence. Illumina developed this array in collaboration with the LUPA Consortium, which includes 22 European universities and other partners such as the Broad Institute.

Learn More About Animal and Plant Genotyping

Specifications:

Input Quantity 200 ng DNA (at 50 ng/ul)

Number of Markers Fixed Markers: 172,115 evenly spaced SNPs across the genome

Method Genome-Wide Genotyping Array

Technology Microarray

Specialized Sample Types FFPE

Species Category Canine

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium OncoArray-500K BeadChip Kit (1152 samples) 500,000 loci. Each package contains 48 BeadChips, along wit

" WG-355-1003 Illumina 产品编号： WG-355-1003美  元  价： $74880.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The Infinium OncoArray-500K contains 500,000 SNPs with a genome-wide backbone of 250,000 tag SNPs. Additional SNPs include genetic variants associated with breast, colorectal, lung, ovarian, and prostate cancers plus SNPs covering ancestry, quantitative traits, pharmacogenetics, and fine-mapping of common cancer susceptibility loci. To support additional research requirements, the Infinium OncoArray-500K can be tailored to incorporate up to 120,000 custom beadtypes.*

The Infinium OncoArray-500K was developed in collaboration with leADIng experts from the OncoArray consortium†, and draws on many of the features of the successful Collaborative Oncological Gene-environment Study (iCOGS) array1. Designed with candidate SNPs identified by the consortium, the OncoArray provides a cost-effective means to assess candidate variants potentially associated with cancer risk.

The Infinium OncoArray-500K uses a 24-sample high-throughput screening (HTS) BeadChip to enable maximum throughput, productivity, and genotyping accuracy. Using the proven iScan System, the Infinium OncoArray-500K is designed to combine affordABIlity with high-density content, providing insight into the relationship between gene variants and cancer predisposition in five of the most prevalent cancers.

* For additional information about custom add-on content, contact your Illumina account manager or sales specialist.

Learn More About Human Genotyping

Specifications:

Input Quantity 200 ng DNA

Sample Throughput ~2,304 samples per week

Number of Markers Fixed Markers: ~500,000u003cbru003e Custom Marker add-on capacity: Up to 120,000 (with + kit versions)

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/PorcineSNP60v2 BeadChip (1152 samples)/WG-410-2003/1 Ea

" WG-410-2003 Illumina 产品编号： WG-410-2003美  元  价： $57600.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The PorcineSNP60 DNA Analysis Kit v2 features 65,000 evenly spaced probes, offering more than sufficient SNP density for whole-genome association studies, determination of genetic merit, identification of quantitative trait loci, and comparative genetic studies.

This 24-sample BeadChip presents a solution for interrogating genetic variation in multiple porcine breeds, including Duroc, Landrace, Pietran, and Large White. A semi-custom version of the BeadChip, the PorcineSNP60+ DNA Analysis Kit v2, allows researchers to include up to 25,000 additional custom probes for targeted studies.

The Porcine SNP Chip Consortium made the final selection of the highest quality Markers from more than 510,000 single nucleotide polymorphisms (SNPs), after combining novel discovered SNPs with several other existing databases and study results.

The panel was optimized using multiple criteria for Marker selection, including minor allele frequency determined from representative sample sequencing, allele count, quality score, spacing, location, and validation status.

Learn More About Plant and Animal Genotyping

Specifications:

Input Quantity 200 ng DNA

Number of Markers Fixed Markers: 64,232u003cbru003eCustom Marker add-on capacity: Up to 25,000 (with + kit versions)

Method Genome-Wide Genotyping Array

Technology Microarray

Specialized Sample Types FFPE

Species Category Porcine

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium® Omni5Exome-4 v1.3 Kit (96 samples)/20005142/1 Ea

" 20005142 Illumina 产品编号： 20005142美  元  价： $26688.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

Infinium Omni5Exome-4 BeadChip delivers comprehensive coverage of the genome, providing functional exonic content for whole-genome genotyping and copy number variation (CNV) analysis. The BeadChips include optimized tag SNPs targeting genetic variation down to 1% minor allele frequency (MAF).

Using the proven iScan array scanner and integrated analysis software, these BeadChips offer optimized tag SNPs, exonic content, and fully supported CNV analysis. Combined with convenient packaging and a streamlined PCR-free protocol, these kits provide a comprehensive DNA analysis solution.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 4,548,474

Sample Throughput ~460 samples per week

Input Quantity 400 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium Exome-24 v1.1 Kit (288 samples)/20015247/1 Ea

" 20015247 Illumina 产品编号： 20015247美  元  价： $13536.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

Infinium Exome-24 BeadChip delivers exceptional coverage of putative functional exonic variants selected from over 12,000 individual exome and whole-genome sequences. Markers were identified through a close collaboration with leADIng geneticists with the goal of developing an extensive catalog of exome variants.

The exonic content consists of >240,000 Markers representing diverse populations - including European, African, Chinese, and Hispanic individuals - and a range of common conditions, such as type 2 diabetes, cancer, metabolic, and psychiatric disorders.

Infinium Exome-24 BeadChip delivers focused coverage of exonic regions, but does not include coverage outside of coding regions. For greater flexibility, the Infinium Exome-24+ version of the BeadChip can be customized to include up to 400,000 additional Markers.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 244,883,Custom Marker add-on capacity: Up to 400,000 (with + kit versions)

Sample Throughput ~2304 samples per week

Input Quantity 200 ng DNA

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/Infinium CoreExome-24 v1.2 Kit (288 samples)/20015263/1 Ea

" 20015263 Illumina 产品编号： 20015263美  元  价： $15552.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Microarray Kits "Product Highlights:

The customizable Infinium CoreExome-24 BeadChip offers an economical way to perform and support large genetic studies, especially large-scale genotyping studies. They can also be used to quickly and easily obtain baseline sample data sets for a variety of downstream applications, including common variant, sex confirmation, mtDNA, ancestry, loss-of-variant, indel, and CNV studies.

Developed in collaboration with several leADIng research institutions, the Infinium CoreExome-24 BeadChip includes all the tag SNPs found on the Infinium Core-24 BeadChip, plus over 240,000 Markers from the Infinium Exome-24 BeadChip. For greater flexibility, the Infinium CoreExome-24+ version of the BeadChip can be customized to include up to 100,000 additional Markers.

The Infinium CoreExome-24 BeadChips use the Infinium high-throughput screening (HTS) 24-sample format, enabling high content flexibility, throughput capacity, and genotyping accuracy. When combined with the proven iScan System, these BeadChips deliver affordable, high-quality, genome-wide information across diverse world populations.

Learn More About Human Genotyping

Specifications:

Number of Markers Fixed Markers: 550,601,Custom Marker add-on capacity: Up to 100,000 (with + kit versions)

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE

Species Category Human

Automation CapABIlity Liquid Handling Robots,Autoloader 2.x Array Loader

System Compatibility iScan"

"Illumina/BaseSpace Sequence Hub Enterprise Annual Subscription/SW-411-1003/1 Ea

" SW-411-1003 Illumina 产品编号： SW-411-1003美  元  价： $29995.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Informatics Products "Product Highlights:

As a key component of the BaseSpace Suite, BaseSpace Sequence Hub is a direct extension of your Illumina instruments. Data flows directly from the instrument into BaseSpace Sequence Hub, which enables you to manage and analyze data using a curated set of analysis apps. BaseSpace Sequence Hub:

Enables you to set up and monitor instrument runs in real-time

Promotes efficiency by converting sequencing data to a standard format and directly streaming them to the cloud

Provides a more economical solution than assembling infrastructures and tools in-house

Increases productivity with easy access and execution of a multitude of genomic analysis apps (provided by you, Illumina, or third-parties). As a result, specialized staff members, such as bioinformaticians are free to work on more complicated analyses

Fosters collaboration and innovation with simplified data sharing

Provides multiple layers of security and supports Health Insurance PortABIlity and AccountABIlity Act (HIPAA) compliance using robust Amazon Web Services cloud infrastructure

Scales to accommodate the need for more storage and computing with a tiered pricing structure

See All BaseSpace Data Analysis Apps

Visit the BaseSpace Suite Blog"

"Illumina/SureRef Reference Female DNA (128 μl)/PR-40-415204-00/1 Ea

" PR-40-415204-00 Illumina 产品编号： PR-40-415204-00美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The SureRef Pack contains both SureRef Reference Male DNA and SureRef Reference Female DNA, each sufficient for 16 reference labeling reactions. The individual DNAs are also available separately.

SureRef DNA products are matched, in terms of quality and size of DNA fragment to a single cell or a few cells. Extracted DNA should be amplified with the SurePlex DNA Amplification System and used as part of 24sure laboratory product protocols."

"Illumina/VeriSeq™ PGS Kit - MiSeq®/RH-101-1001/1 Ea

" RH-101-1001 Illumina 产品编号： RH-101-1001美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The VeriSeq PGS Kit takes advantage of next-generation sequencing (NGS) technology to provide comprehensive, accurate screening of all 24 chromosomes for selection of embryos most likely to be euploid. Preimplantation genetic screening (PGS) results generated using VeriSeq PGS are comparable to those achieved with the widely used array-based 24sure technology. In addition, NGS offers the opportunity for improved assay workflow, higher throughput, and enhanced performance.1

Industry-leADIng data quality: More than 90% of the world’s sequencing data is generated by Illumina sequencing by synthesis (SBS) chemistry*

Fast, streamlined workflow: Sample to answer in approximately 12 hours

High-throughput analysis: Screen up to 24 samples per run

Ultra-Low Input

NGS offers a highly sensitive method for screening embryos, requiring as little as 1 ng of DNA from a SurePlex DNA amplification reaction. DNA can be obtained from a blastomere biopsy, from a day 3 embryo, or from a trophectodermal (TE) biopsy from a blastocyst.

*Data calculations on file. Illumina, Inc., 2015.

Specifications:

Assay Time 11 hours

Hands-On Time 4 hours

Input Quantity 30 to 300 ng depending on QC results

System Compatibility MiSeq,MiSeqDx in Research Mode

Method PGS Sequencing

Technology Sequencing

Specialized Sample Types Single Cells

Species Category Human

Variant Class Chromosomal Abnormalities,Structural Variants,Copy Number Variants (CNVs)"

"Illumina/Infinium CytoSNP-850K v1.1 BeadChip Kit (48 samples)/WG-322-1103/1 Ea

" WG-322-1103 Illumina 产品编号： WG-322-1103美  元  价： $12000.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The Infinium CytoSNP-850K v1.1 BeadChip is the first SNP-based human microarray to leverage the latest input from the international community for cytogenomics studies. The gene list was defined based on content from the International Collaboration for Clinical Genomics (ICCG)1 and the Cancer Genomics Consortium (CGC)2, providing a comprehensive view of cytogenomic activity.* This enhanced SNP coverage enables copy number calls as small as 10 kb in regions associated with genetic disease.

Thorough Coverage for Constitutional and Cancer Applications

The Infinium CytoSNP-850K v1.1 BeadChip provides comprehensive coverage of cytogenetically relevant genes on a proven platform, helping researchers find valuable information that may be missed by other technologies. It contains approximately 850,000 empirically selected single nucleotide polymorphisms (SNPs) spanning the entire genome with enriched coverage for 3,262 genes of known cytogenetics relevance in both constitutional and cancer applications.

High Detection Sensitivity for Low-level Mosaics

A unique attribute of the Infinium array technology is the use of long 50-mer probes that have high specificity to the SNP target. This increases sensitivity for low-level mosaics3 and offers high resolution for copy-neutral loss of heterozygosity (LOH). In addition, each SNP is represented in at least 15x redundancy to increase the signal-to-noise ratio for making accurate copy number variation (CNV) and absence of heterozygosity (AOH) calls across the genome. As few as 10 consecutive probes are necessary to make accurate CNV calls, demonstrating the high performance level of the Infinium assay.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,6 hours 15 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

Specialized Sample Types FFPE

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

"Illumina/Infinium CytoSNP-850K v1.1 BeadChip Kit (96 samples)/WG-322-1104/1 Ea

" WG-322-1104 Illumina 产品编号： WG-322-1104美  元  价： $24000.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The Infinium CytoSNP-850K v1.1 BeadChip is the first SNP-based human microarray to leverage the latest input from the international community for cytogenomics studies. The gene list was defined based on content from the International Collaboration for Clinical Genomics (ICCG)1 and the Cancer Genomics Consortium (CGC)2, providing a comprehensive view of cytogenomic activity.* This enhanced SNP coverage enables copy number calls as small as 10 kb in regions associated with genetic disease.

Thorough Coverage for Constitutional and Cancer Applications

The Infinium CytoSNP-850K v1.1 BeadChip provides comprehensive coverage of cytogenetically relevant genes on a proven platform, helping researchers find valuable information that may be missed by other technologies. It contains approximately 850,000 empirically selected single nucleotide polymorphisms (SNPs) spanning the entire genome with enriched coverage for 3,262 genes of known cytogenetics relevance in both constitutional and cancer applications.

High Detection Sensitivity for Low-level Mosaics

A unique attribute of the Infinium array technology is the use of long 50-mer probes that have high specificity to the SNP target. This increases sensitivity for low-level mosaics3 and offers high resolution for copy-neutral loss of heterozygosity (LOH). In addition, each SNP is represented in at least 15x redundancy to increase the signal-to-noise ratio for making accurate copy number variation (CNV) and absence of heterozygosity (AOH) calls across the genome. As few as 10 consecutive probes are necessary to make accurate CNV calls, demonstrating the high performance level of the Infinium assay.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,6 hours 15 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

Specialized Sample Types FFPE

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

"Illumina/24sure Microarray Pack (16 hybridizations, up to 32 samples)/PR-10-408702-PK/1 Ea

" PR-10-408702-PK Illumina 产品编号： PR-10-408702-PK美  元  价： $3530.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Preimplantation genetic screening (PGS) with 24sure microarrays provides a comprehensive and accurate assessment of all 24 chromosomes from an embryo biopsy. The arrays can be used to screen for aneuploidy in first and second polar bodies, blastomere biopsies, and trophectoderm biopsies. The 24sure assay is objective, ensuring a high degree of confidence in the results and subsequent selection of embryos least likely to have aneuploidy for transfer.

Widely used, proven PGS – Method of choice for many of the world’s reference laboratories

Fast, reliable results – Comprehensive, accurate assessment of all 24 chromosomes in 12 hours

Straightforward workflow – Optimized protocols include minimal tube transfers, documented quality control (QC) stages, and flexIBLe stopping points

Straightforward Workflow Is Complete in 12 Hours

The straightforward 24sure protocol used standard laboratory techniques, providing a fast, accurate method for chromosome screening with minimal sensitivity to technical errors and designed to fit into existing workflows. Screening can be completed in 12 hours, and therefore does not interfere with tight schedules of a fresh IVF cycle.

Specifications:

Assay Time ~12 hours

Hands-On Time ~2.5 hours

Input Quantity Single cell or multi-cell samples

Technology Microarray

Specialized Sample Types Single Cells

Species Category Human

Variant Class Chromosomal Abnormalities,Structural Variants,Copy Number Variants (CNVs)

Method PGS Array

Significantly Higher Rate of Pregnancy May Be PossIBLe with PGS:

The pregnancy rate shown for embryos analyzed using preimplantation genetic screening and morphology is 69.1%, much higher than the 41.7% achieved using morphology screening alone.1 Pilot study of young, good prognosis patients (age < 35, first-time IVF, no history of prior miscarriage, n = 103).

PGS with 24sure Arrays Reduces the Effect of Maternal Age on Implantation and Pregnancy Rates:

Although aneuploidy risk increases with maternal age, PGS using 24sure arrays on either day 3 or day 5 embryos diminishes the effect of maternal age on implantation and pregnancy rates.2

24Sure Laboratory Workflow:

The 24sure laboratory workflow is a straightforward procedure that is completed in <12 hours.

BlueFuse Multi Software Delivers a Complete Data Analysis and Information Management System:

BlueFuse Multi Software provides a complete solution for analyzing, storing, and reporting 24sure results. A. Sample database displays experimental information. B. Array images imported directly from the scanner. C. Profiles for sample and reference chromosomes. D. Automated cycle reports."

"Illumina/HumanCytoSNP FFPE-12 v2.1 DNA Analysis BeadChip Kit (24 samples) 250,000 loci. Each package contains 2 BeadChip

" WG-321-1003 Illumina 产品编号： WG-321-1003美  元  价： $3720.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The HumanCytoSNP FFPE-12 BeadChip interrogates >262,000 genomic Markers. The content consists of intelligently selected tag SNPs that provide comprehensive genomic coverage.

With dense and even Marker spacing, the BeadChip content can also be used to precisely detect structural aberrations, allowing researchers to accurately identify structural differences between paired-normal and tumor samples.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,2 hours 10 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

Specialized Sample Types FFPE

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

"Illumina/24sure+ Pack (16 samples)/PR-10-408602-PK/1 Ea

" PR-10-408602-PK Illumina 产品编号： PR-10-408602-PK美  元  价： $4460.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The 24sure+ microarray is designed to improve the success of in vitro fertilization (IVF) by detecting subchromosomal imbalances in embryos of reciprocal translocation carriers. 24sure+ includes increased coverage of pericentromeric and subtelomeric regions to ensure the identification and reporting of small derived chromosome fragments from single cells.

24sure+ microarrays offer several key advantages:

24sure+ can more accurately determine balanced or euploid embryos for selection compared to trADItional fluorescent in situ hybridization (FISH) methods1

Rapid workflow enables sample results and reporting within 12 hours

Every 24sure+ purchase includes BlueFuse Multi software for the fully automated analysis, interpretation, and reporting of 24sure+ investigations

24sure+ analysis is ideally offered to couples undergoing IVF due to recurrent miscarriages sUSPected to be caused by the presence of a balanced translocation in either parent. Balanced translocation carriers may not present with a phenotype. However, segregation of the translocated chromosomes during gamete formation can produce a range of normal, balanced, and unbalanced gametes, with unbalanced products expected to represent 50% of gametes.2 Resulting embryos with large structural imbalances are likely to be non-viable. As a result, the balanced carrier may present with infertility or a history of recurrent miscarriage/spontaneous abortion.3

Couples presenting with recurrent miscarriage can be karyotyped, which is a low-resolution analysis that may determine whether the problems they are experiencing are due to a balanced rearrangement. If this is the case, such carriers of balanced translocations may seek 24sure+ analysis of embryos.

Specifications:

Assay Time ~12 hours

Hands-On Time ~3.5 hours

Input Quantity SurePlex whole-genome-amplified single cells from embryo biopsies

Technology Microarray

Method PGD Array

Specialized Sample Types Single Cells

Species Category Human

Variant Class Chromosomal Abnormalities"

"Illumina/HumanCytoSNP-12 v2.1 BeadChip Kit (12 samples)/WG-320-2101/1 Ea

" WG-320-2101 Illumina 产品编号： WG-320-2101美  元  价： $1860.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The 12-sample HumanCytoSNP-12 BeadChip is a powerful, whole-genome scanning panel designed for efficient, high-throughput analysis of genetic and structural variations that are most relevant to human disease. This scalable, proven solution offers substantially better resolution to detect smaller regions than fluorescence in situ hybridization (FISH) or comparative genomic hybridization (CGH).1

Complete panel of genome-wide tag single nucleotide polymorphisms (SNPs) and Markers targeting all regions of known cytogenetic importance, incorporating ~300,000 “best of the best” SNPs with the highest tagging power

Average SNP call rates and reproducibility of > 99.9%, and low noise for copy number measurements

Detect many types and sizes of structural variation in the human genome that affect phenotypes, including duplications, deletions, amplifications, copy-neutral loss of heterozygosity (LOH), and mosaicism

Process up to 12 samples in parallel, increasing sample throughput and decreasing experimental variABIlity

Process samples using the single-tube, PCR-free Infinium HD Assay that requires only 200ng DNA per sample

Comprehensive data analysis with intuitive BlueFuse Multi Software streamlines copy number assessment and results reporting

The HumanCytoSNP-12 BeadChip is optimized to detect cytogenetic abnormalities most relevant to human disease.1-2 Content includes ~300,000 SNPs targeting regions shown to be important for cytogenetic analysis. The result is dense coverage of ~250 disease regions, including subtelomeric regions, pericentromeric regions, and sex chromosomes, commonly screened in cytogenetics labs. Sufficient SNP coverage is provided to determine dosage sensitivity of > 800 genes.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,6 hours 15 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

Illumina/Infinium CytoSNP-850K v1.1 BeadChip Kit (8 samples)/WG-322-1101/1 Ea WG-322-1101 Illumina 产品编号： WG-322-1101美  元  价： $2000.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The Infinium CytoSNP-850K v1.1 BeadChip is the first SNP-based human microarray to leverage the latest input from the international community for cytogenomics studies. The gene list was defined based on content from the International Collaboration for Clinical Genomics (ICCG)1 and the Cancer Genomics Consortium (CGC)2, providing a comprehensive view of cytogenomic activity.* This enhanced SNP coverage enables copy number calls as small as 10 kb in regions associated with genetic disease.

Thorough Coverage for Constitutional and Cancer Applications

The Infinium CytoSNP-850K v1.1 BeadChip provides comprehensive coverage of cytogenetically relevant genes on a proven platform, helping researchers find valuable information that may be missed by other technologies. It contains approximately 850,000 empirically selected single nucleotide polymorphisms (SNPs) spanning the entire genome with enriched coverage for 3,262 genes of known cytogenetics relevance in both constitutional and cancer applications.

High Detection Sensitivity for Low-level Mosaics

A unique attribute of the Infinium array technology is the use of long 50-mer probes that have high specificity to the SNP target. This increases sensitivity for low-level mosaics3 and offers high resolution for copy-neutral loss of heterozygosity (LOH). In addition, each SNP is represented in at least 15x redundancy to increase the signal-to-noise ratio for making accurate copy number variation (CNV) and absence of heterozygosity (AOH) calls across the genome. As few as 10 consecutive probes are necessary to make accurate CNV calls, demonstrating the high performance level of the Infinium assay.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,6 hours 15 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

Specialized Sample Types FFPE

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

"Illumina/Infinium CytoSNP-850K v1.1 BeadChip Kit (16 samples)/WG-322-1102/1 Ea

" WG-322-1102 Illumina 产品编号： WG-322-1102美  元  价： $4000.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The Infinium CytoSNP-850K v1.1 BeadChip is the first SNP-based human microarray to leverage the latest input from the international community for cytogenomics studies. The gene list was defined based on content from the International Collaboration for Clinical Genomics (ICCG)1 and the Cancer Genomics Consortium (CGC)2, providing a comprehensive view of cytogenomic activity.* This enhanced SNP coverage enables copy number calls as small as 10 kb in regions associated with genetic disease.

Thorough Coverage for Constitutional and Cancer Applications

The Infinium CytoSNP-850K v1.1 BeadChip provides comprehensive coverage of cytogenetically relevant genes on a proven platform, helping researchers find valuable information that may be missed by other technologies. It contains approximately 850,000 empirically selected single nucleotide polymorphisms (SNPs) spanning the entire genome with enriched coverage for 3,262 genes of known cytogenetics relevance in both constitutional and cancer applications.

High Detection Sensitivity for Low-level Mosaics

A unique attribute of the Infinium array technology is the use of long 50-mer probes that have high specificity to the SNP target. This increases sensitivity for low-level mosaics3 and offers high resolution for copy-neutral loss of heterozygosity (LOH). In addition, each SNP is represented in at least 15x redundancy to increase the signal-to-noise ratio for making accurate copy number variation (CNV) and absence of heterozygosity (AOH) calls across the genome. As few as 10 consecutive probes are necessary to make accurate CNV calls, demonstrating the high performance level of the Infinium assay.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,6 hours 15 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

Specialized Sample Types FFPE

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

"Illumina/HumanCytoSNP FFPE-12 v2.1 DNA Analysis BeadChip Kit (48 samples) 250,000 loci. Each package contains 4 BeadChip

" WG-321-1004 Illumina 产品编号： WG-321-1004美  元  价： $6960.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The HumanCytoSNP FFPE-12 BeadChip interrogates >262,000 genomic Markers. The content consists of intelligently selected tag SNPs that provide comprehensive genomic coverage.

With dense and even Marker spacing, the BeadChip content can also be used to precisely detect structural aberrations, allowing researchers to accurately identify structural differences between paired-normal and tumor samples.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,2 hours 10 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

Specialized Sample Types FFPE

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

Illumina/HumanKaryomap-12 DNA Analysis Kit (24 samples)/RH-103-1001/1 Ea RH-103-1001 Illumina 产品编号： RH-103-1001美  元  价： $3758.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The HumanKaryomap-12 DNA Analysis Kit is a comprehensive genome-wide test available at the single-cell level. It provides insight into the inheritance of single-gene defects.

This BeadChip array targets ~300,000 of the most informative Markers in the genome for efficient genome-wide coverage. Karyomapping uses bioMarkers within the genome to assess the likelihood of an embryo carrying a gene variant involved in a single-gene disorder.

Preimplantation genetic diagnosis (PGD) with karyomapping enables screening of embryos for the likelihood of carrying the defective gene prior to transfer to the uterus. This method uses SNP (single nucleotide polymorphisms) array technology to confirm the presence or absence of a specific allele.

Specifications:

Assay Time ~2 days (shortened Infinium protocol)

Hands-on Time ~4 hours

Input Quantity 400 ng genomic DNA from parent and reference samples + SureMDA whole-genome-amplified single cells from embryo biopsy samples

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs)

System Compatibility NextSeq 550,iScan

Method PGD Array

Specialized Sample Types Single Cells

Species Category Human"

Illumina/SureRef Reference Male DNA (128 ?l)/PR-40-415203-00/1 Ea PR-40-415203-00 Illumina 产品编号： PR-40-415203-00美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The SureRef Pack contains both SureRef Reference Male DNA and SureRef Reference Female DNA, each sufficient for 16 reference labeling reactions. The individual DNAs are also available separately.

SureRef DNA products are matched, in terms of quality and size of DNA fragment to a single cell or a few cells. Extracted DNA should be amplified with the SurePlex DNA Amplification System and used as part of 24sure laboratory product protocols."

"Illumina/SurePlex DNA Amplification System (50 reactions)/PR-40-415101-00/1 Ea

" PR-40-415101-00 Illumina 产品编号： PR-40-415101-00美  元  价： $1380.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Preimplantation genetic screening (PGS) with 24sure microarrays provides a comprehensive and accurate assessment of all 24 chromosomes from an embryo biopsy. The arrays can be used to screen for aneuploidy in first and second polar bodies, blastomere biopsies, and trophectoderm biopsies. The 24sure assay is objective, ensuring a high degree of confidence in the results and subsequent selection of embryos least likely to have aneuploidy for transfer.

Widely used, proven PGS – Method of choice for many of the world’s reference laboratories

Fast, reliable results – Comprehensive, accurate assessment of all 24 chromosomes in 12 hours

Straightforward workflow – Optimized protocols include minimal tube transfers, documented quality control (QC) stages, and flexIBLe stopping points

Straightforward Workflow Is Complete in 12 Hours

The straightforward 24sure protocol used standard laboratory techniques, providing a fast, accurate method for chromosome screening with minimal sensitivity to technical errors and designed to fit into existing workflows. Screening can be completed in 12 hours, and therefore does not interfere with tight schedules of a fresh IVF cycle.

Specifications:

Assay Time ~12 hours

Hands-On Time ~2.5 hours

Input Quantity Single cell or multi-cell samples

Technology Microarray

Specialized Sample Types Single Cells

Species Category Human

Variant Class Chromosomal Abnormalities,Structural Variants,Copy Number Variants (CNVs)

Method PGS Array

Significantly Higher Rate of Pregnancy May Be PossIBLe with PGS:

The pregnancy rate shown for embryos analyzed using preimplantation genetic screening and morphology is 69.1%, much higher than the 41.7% achieved using morphology screening alone.1 Pilot study of young, good prognosis patients (age < 35, first-time IVF, no history of prior miscarriage, n = 103).

PGS with 24sure Arrays Reduces the Effect of Maternal Age on Implantation and Pregnancy Rates:

Although aneuploidy risk increases with maternal age, PGS using 24sure arrays on either day 3 or day 5 embryos diminishes the effect of maternal age on implantation and pregnancy rates.2

24Sure Laboratory Workflow:

The 24sure laboratory workflow is a straightforward procedure that is completed in <12 hours.

BlueFuse Multi Software Delivers a Complete Data Analysis and Information Management System:

BlueFuse Multi Software provides a complete solution for analyzing, storing, and reporting 24sure results. A. Sample database displays experimental information. B. Array images imported directly from the scanner. C. Profiles for sample and reference chromosomes. D. Automated cycle reports."

"Illumina/HumanCytoSNP-12 v2.1 BeadChip Kit (288 samples)/WG-320-2104/1 Ea

" WG-320-2104 Illumina 产品编号： WG-320-2104美  元  价： $41760.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The 12-sample HumanCytoSNP-12 BeadChip is a powerful, whole-genome scanning panel designed for efficient, high-throughput analysis of genetic and structural variations that are most relevant to human disease. This scalable, proven solution offers substantially better resolution to detect smaller regions than fluorescence in situ hybridization (FISH) or comparative genomic hybridization (CGH).1

Complete panel of genome-wide tag single nucleotide polymorphisms (SNPs) and Markers targeting all regions of known cytogenetic importance, incorporating ~300,000 “best of the best” SNPs with the highest tagging power

Average SNP call rates and reproducibility of > 99.9%, and low noise for copy number measurements

Detect many types and sizes of structural variation in the human genome that affect phenotypes, including duplications, deletions, amplifications, copy-neutral loss of heterozygosity (LOH), and mosaicism

Process up to 12 samples in parallel, increasing sample throughput and decreasing experimental variABIlity

Process samples using the single-tube, PCR-free Infinium HD Assay that requires only 200ng DNA per sample

Comprehensive data analysis with intuitive BlueFuse Multi Software streamlines copy number assessment and results reporting

The HumanCytoSNP-12 BeadChip is optimized to detect cytogenetic abnormalities most relevant to human disease.1-2 Content includes ~300,000 SNPs targeting regions shown to be important for cytogenetic analysis. The result is dense coverage of ~250 disease regions, including subtelomeric regions, pericentromeric regions, and sex chromosomes, commonly screened in cytogenetics labs. Sufficient SNP coverage is provided to determine dosage sensitivity of > 800 genes.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,6 hours 15 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

"Illumina/SureMDA DNA Amplification System (96 reactions)/PR-40-405102-00/1 Ea

" PR-40-405102-00 Illumina 产品编号： PR-40-405102-00美  元  价： $2920.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The HumanKaryomap-12 DNA Analysis Kit is a comprehensive genome-wide test available at the single-cell level. It provides insight into the inheritance of single-gene defects.

This BeadChip array targets ~300,000 of the most informative Markers in the genome for efficient genome-wide coverage. Karyomapping uses bioMarkers within the genome to assess the likelihood of an embryo carrying a gene variant involved in a single-gene disorder.

Preimplantation genetic diagnosis (PGD) with karyomapping enables screening of embryos for the likelihood of carrying the defective gene prior to transfer to the uterus. This method uses SNP (single nucleotide polymorphisms) array technology to confirm the presence or absence of a specific allele.

Specifications:

Assay Time ~2 days (shortened Infinium protocol)

Hands-on Time ~4 hours

Input Quantity 400 ng genomic DNA from parent and reference samples + SureMDA whole-genome-amplified single cells from embryo biopsy samples

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs)

System Compatibility NextSeq 550,iScan

Method PGD Array

Specialized Sample Types Single Cells

Species Category Human"

"Illumina/HumanCytoSNP-12 v2.1 BeadChip Kit (24 samples)/WG-320-2102/1 Ea

" WG-320-2102 Illumina 产品编号： WG-320-2102美  元  价： $3720.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The 12-sample HumanCytoSNP-12 BeadChip is a powerful, whole-genome scanning panel designed for efficient, high-throughput analysis of genetic and structural variations that are most relevant to human disease. This scalable, proven solution offers substantially better resolution to detect smaller regions than fluorescence in situ hybridization (FISH) or comparative genomic hybridization (CGH).1

Complete panel of genome-wide tag single nucleotide polymorphisms (SNPs) and Markers targeting all regions of known cytogenetic importance, incorporating ~300,000 “best of the best” SNPs with the highest tagging power

Average SNP call rates and reproducibility of > 99.9%, and low noise for copy number measurements

Detect many types and sizes of structural variation in the human genome that affect phenotypes, including duplications, deletions, amplifications, copy-neutral loss of heterozygosity (LOH), and mosaicism

Process up to 12 samples in parallel, increasing sample throughput and decreasing experimental variABIlity

Process samples using the single-tube, PCR-free Infinium HD Assay that requires only 200ng DNA per sample

Comprehensive data analysis with intuitive BlueFuse Multi Software streamlines copy number assessment and results reporting

The HumanCytoSNP-12 BeadChip is optimized to detect cytogenetic abnormalities most relevant to human disease.1-2 Content includes ~300,000 SNPs targeting regions shown to be important for cytogenetic analysis. The result is dense coverage of ~250 disease regions, including subtelomeric regions, pericentromeric regions, and sex chromosomes, commonly screened in cytogenetics labs. Sufficient SNP coverage is provided to determine dosage sensitivity of > 800 genes.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,6 hours 15 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

"Illumina/TruSight Cardio Sequencing Kit for NextSeq (48 indexes, 48 samples, 4 enrichments)/FC-141-1011/1 Ea

" FC-141-1011 Illumina 产品编号： FC-141-1011美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Expertly defined genes selected in collaboration with the Imperial College of London

99% of the targeted regions covered at a depth of at least 20×*

Cost to sequence is ~ $1 US per gene; fully supported on Illumina sequencing and informatics platforms

The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart and Lung Institute at the Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1

Specifications:

Input Quantity 50 ng DNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Method Targeted DNA Sequencing"

"Illumina/MiSeq® Reagent Kit v3 - PGS/RH-102-1001/1 Ea

" RH-102-1001 Illumina 产品编号： RH-102-1001美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The VeriSeq PGS Kit takes advantage of next-generation sequencing (NGS) technology to provide comprehensive, accurate screening of all 24 chromosomes for selection of embryos most likely to be euploid. Preimplantation genetic screening (PGS) results generated using VeriSeq PGS are comparable to those achieved with the widely used array-based 24sure technology. In addition, NGS offers the opportunity for improved assay workflow, higher throughput, and enhanced performance.1

Industry-leADIng data quality: More than 90% of the world’s sequencing data is generated by Illumina sequencing by synthesis (SBS) chemistry*

Fast, streamlined workflow: Sample to answer in approximately 12 hours

High-throughput analysis: Screen up to 24 samples per run

Ultra-Low Input

NGS offers a highly sensitive method for screening embryos, requiring as little as 1 ng of DNA from a SurePlex DNA amplification reaction. DNA can be obtained from a blastomere biopsy, from a day 3 embryo, or from a trophectodermal (TE) biopsy from a blastocyst.

*Data calculations on file. Illumina, Inc., 2015.

Specifications:

Assay Time 11 hours

Hands-On Time 4 hours

Input Quantity 30 to 300 ng depending on QC results

System Compatibility MiSeq,MiSeqDx in Research Mode

Method PGS Sequencing

Technology Sequencing

Specialized Sample Types Single Cells

Species Category Human

Variant Class Chromosomal Abnormalities,Structural Variants,Copy Number Variants (CNVs)"

"Illumina/TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (12 indexes, 12 samples, 1 enrichment)/FC-141-1010/1 Ea

" FC-141-1010 Illumina 产品编号： FC-141-1010美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Expertly defined genes selected in collaboration with the Imperial College of London

99% of the targeted regions covered at a depth of at least 20×*

Cost to sequence is ~ $1 US per gene; fully supported on Illumina sequencing and informatics platforms

The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart and Lung Institute at the Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1

Specifications:

Input Quantity 50 ng DNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Method Targeted DNA Sequencing"

"Illumina/SureRef Pack (16 reference hybridizations)/PR-40-415205-PK/1 Ea

" PR-40-415205-PK Illumina 产品编号： PR-40-415205-PK美  元  价： $349.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Preimplantation genetic screening (PGS) with 24sure microarrays provides a comprehensive and accurate assessment of all 24 chromosomes from an embryo biopsy. The arrays can be used to screen for aneuploidy in first and second polar bodies, blastomere biopsies, and trophectoderm biopsies. The 24sure assay is objective, ensuring a high degree of confidence in the results and subsequent selection of embryos least likely to have aneuploidy for transfer.

Widely used, proven PGS – Method of choice for many of the world’s reference laboratories

Fast, reliable results – Comprehensive, accurate assessment of all 24 chromosomes in 12 hours

Straightforward workflow – Optimized protocols include minimal tube transfers, documented quality control (QC) stages, and flexIBLe stopping points

Straightforward Workflow Is Complete in 12 Hours

The straightforward 24sure protocol used standard laboratory techniques, providing a fast, accurate method for chromosome screening with minimal sensitivity to technical errors and designed to fit into existing workflows. Screening can be completed in 12 hours, and therefore does not interfere with tight schedules of a fresh IVF cycle.

Specifications:

Assay Time ~12 hours

Hands-On Time ~2.5 hours

Input Quantity Single cell or multi-cell samples

Technology Microarray

Specialized Sample Types Single Cells

Species Category Human

Variant Class Chromosomal Abnormalities,Structural Variants,Copy Number Variants (CNVs)

Method PGS Array

Significantly Higher Rate of Pregnancy May Be PossIBLe with PGS:

The pregnancy rate shown for embryos analyzed using preimplantation genetic screening and morphology is 69.1%, much higher than the 41.7% achieved using morphology screening alone.1 Pilot study of young, good prognosis patients (age < 35, first-time IVF, no history of prior miscarriage, n = 103).

PGS with 24sure Arrays Reduces the Effect of Maternal Age on Implantation and Pregnancy Rates:

Although aneuploidy risk increases with maternal age, PGS using 24sure arrays on either day 3 or day 5 embryos diminishes the effect of maternal age on implantation and pregnancy rates.2

24Sure Laboratory Workflow:

The 24sure laboratory workflow is a straightforward procedure that is completed in <12 hours.

BlueFuse Multi Software Delivers a Complete Data Analysis and Information Management System:

BlueFuse Multi Software provides a complete solution for analyzing, storing, and reporting 24sure results. A. Sample database displays experimental information. B. Array images imported directly from the scanner. C. Profiles for sample and reference chromosomes. D. Automated cycle reports."

"Illumina/HumanCytoSNP-12 v2.1 BeadChip Kit (48 samples)/WG-320-2103/1 Ea

" WG-320-2103 Illumina 产品编号： WG-320-2103美  元  价： $6960.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The 12-sample HumanCytoSNP-12 BeadChip is a powerful, whole-genome scanning panel designed for efficient, high-throughput analysis of genetic and structural variations that are most relevant to human disease. This scalable, proven solution offers substantially better resolution to detect smaller regions than fluorescence in situ hybridization (FISH) or comparative genomic hybridization (CGH).1

Complete panel of genome-wide tag single nucleotide polymorphisms (SNPs) and Markers targeting all regions of known cytogenetic importance, incorporating ~300,000 “best of the best” SNPs with the highest tagging power

Average SNP call rates and reproducibility of > 99.9%, and low noise for copy number measurements

Detect many types and sizes of structural variation in the human genome that affect phenotypes, including duplications, deletions, amplifications, copy-neutral loss of heterozygosity (LOH), and mosaicism

Process up to 12 samples in parallel, increasing sample throughput and decreasing experimental variABIlity

Process samples using the single-tube, PCR-free Infinium HD Assay that requires only 200ng DNA per sample

Comprehensive data analysis with intuitive BlueFuse Multi Software streamlines copy number assessment and results reporting

The HumanCytoSNP-12 BeadChip is optimized to detect cytogenetic abnormalities most relevant to human disease.1-2 Content includes ~300,000 SNPs targeting regions shown to be important for cytogenetic analysis. The result is dense coverage of ~250 disease regions, including subtelomeric regions, pericentromeric regions, and sex chromosomes, commonly screened in cytogenetics labs. Sufficient SNP coverage is provided to determine dosage sensitivity of > 800 genes.

Specifications:

Assay Time 3 days

Hands-On Time 1 hour 5 min. for automated 8-beadchip workflow,6 hours 15 min. for manual 8-beadchip workflow

Input Quantity 200 ng DNA

Method Genome-Wide Genotyping Array,Cytogenomic Array

Technology Microarray

Variant Class Single Nucleotide Polymorphisms (SNPs),Loss of Heterozygosity (LOH),Chromosomal Abnormalities,Copy Number Variants (CNVs)

System Compatibility NextSeq 550,iScan

Species Category Human

Cancer Type Hematological,Solid Tumor"

"Illumina/Ribo-Zero rRNA Removal Kit (Bacteria) 24 reactions 99% of rRNA from Gram-negative and Gram-positive bacterial R

" MRZB12424 Illumina 产品编号： MRZB12424美  元  价： $2066.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Removes ribosomal RNA (rRNA) from intact and partially degraded mixtures of Gram (+) and Gram (–) bacteria in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for small sample quantities – only 1 μg of total RNA required

Virtually all rRNA is depleted from intact and partially degraded samples in 2 hours.

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with the Ribo-Zero rRNA Removal Kit (Bacteria) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants and isoforms.

This kit offers informative sequencing results by removing unwanted cytoplasmic (nuclear-encoded) rRNA from bacterial mixtures prior to sequencing."

"Illumina/Globin-Zero Gold rRNA Removal Kit 6 reactions 99% of rRNA and >99% of globin mRNA from 1 µg - 5 µg of blood to

" GZG1206 Illumina 产品编号： GZG1206美  元  价： $567.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Remove globin mRNA and ribosomal RNA (rRNA) in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small sample quantities – only 1 μg of total RNA required

Blood is an important sample for research into thousands of rare diseases and other disorders. The sequencing data from samples treated with Globin-Zero are focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms. Globin-Zero removes unwanted globin mRNA, as well as cytoplasmic and mitochondrial rRNA, from mammalian blood RNA samples before sequencing library construction.

Alternative kits

ScriptSeq Complete Gold Kit (Blood): Recommended for sequencing low input samples (e.g. 100 ng of total RNA)

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs

Globin-Zero Gold removes globin mRNA, as well as rRNA from nuclear and mitochondrial regions:

Globin-Zero Gold depletes unwanted globin mRNA and ribosomal RNA from total RNA extracted from blood. Removal of these unwanted subunits produces sequencing results that are heavily focused on BIOLOGically informative genomic regions.

Globin-Zero Gold efficiently removes both globin mRNA and rRNA:

Removal of these unwanted subunits allows generation of sequencing libraries that produce data heavily focused on BIOLOGically informative genetic regions."

"Illumina/Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) 6 reactions 99% of rRNA from 1-5 µg of Gram-positive bacter

" MRZGP126 Illumina 产品编号： MRZGP126美  元  价： $582.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Removes ribosomal RNA (rRNA) from intact and partially degraded Gram (+) bacteria

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for small sample quantities – only 1 μg of total RNA required

Nuclear rRNA removed: 23S, 16S, 5S

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) are focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

The Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) offers informative sequencing results by removing unwanted rRNA prior to sequencing. The kit removes rRNA from both intact and partially degraded samples.

Removes all major rRNA subunits

The Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) depletes unwanted rRNA from total RNA. Removal of these unwanted subunits produces sequencing results that are heavily focused on BIOLOGically informative genomic regions."

"Illumina/Ribo-Zero Gold rRNA Removal Kit (Epidemiology) 6 reactions/MRZE706/1 Ea

" MRZE706 Illumina 产品编号： MRZE706美  元  价： $582.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Removes cytoplasmic and mitochondrial ribosomal RNA (rRNA) from samples composed of human/mouse/rat, Gram-positive and Gram-negative bacterial RNA (see table)

Requires 500 ng to 2.5 μg total RNA per sample

rRNA removal from both intact and partially degraded RNA

Single-pass, 90- to 120-minute process

Recovers all fragments of human and bacterial non-ribosomal RNAs

Applicable to a broad spectrum of bacteria

The Ribo-Zero Gold rRNA Removal Kit (Epidemiology) combines Ribo-Zero Gold (Human/Mouse/Rat) and Ribo-Zero (Bacteria) rRNA removal reagents into a single solution that effectively removes rRNA, including human mitochondrial RNA, from samples composed of both human/mouse/rat and bacterial RNA.

Hypothesis-free NGS is more sensitive to diversity than 16S profiling

Ribo-Zero Gold rRNA Removal Kit (Epidemiology) has increased sensitivity to BIOLOGical diversity because it captures all metatranscriptomic information (instead of directed capture with 16S profiling).

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Related Information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs

Specifications:

Technology Sequencing

Species Category Human,Mouse,Rat,Bacteria

Specialized Sample Types FFPE

System Compatibility Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000"

"Illumina/Ribo-Zero rRNA Removal Kit (Plant Leaf) 6 reactions/MRZPL116/1 Ea

" MRZPL116 Illumina 产品编号： MRZPL116美  元  价： $582.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Removes cytoplasmic, mitochondrial, and chloroplast ribosomal RNA (rRNA) in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small sample quantities – only 1 μg of total RNA required

Virtually all rRNA is depleted from intact and partially degraded samples in 2 hours.

Sequencing is an important discovery tool for plant research. The sequencing data from samples treated with Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) offer informative sequencing results by removing unwanted cytoplasmic, mitochondrial, and chloroplast rRNA prior to sequencing. In order to achieve maximum depletion of rRNA, the Ribo-Zero (Plant Leaf) kit contains a higher percentage of probes to deplete chloroplast rRNA compared to Ribo-Zero (Plant Seed/Root).

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kits

ScriptSeq Complete Kit (Plant): Recommended for low input plant samples (e.g. 100 ng of total RNA).

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs"

"Illumina/Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) 6 reactions 99% of rRNA from 1- 5 µg of Gram-negative bacte

" MRZGN126 Illumina 产品编号： MRZGN126美  元  价： $582.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Remove ribosomal RNA (rRNA) from intact and partially degraded Gram (-) bacteria

Sequencing data contain complete transcriptome of coding and noncoding RNA species

Good for small sample quantities – only 1 μg of total RNA required

Nuclear rRNA removed: 23S, 16S, 5S

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with the Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) are focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

The Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) offers informative sequencing results by removing unwanted rRNA prior to sequencing. The kit removes rRNA from intact and partially degraded samples.

Removes all major rRNA subunits

The Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) depletes unwanted rRNA from total RNA. Removal of these unwanted subunits produces sequencing results that are heavily focused on BIOLOGically informative genomic regions."

"Illumina/Globin-Zero Gold rRNA Removal Kit 24 reactions 99% of rRNA and >99% of globin mRNA from 1 µg - 5 µg of blood t

" GZG1224 Illumina 产品编号： GZG1224美  元  价： $2016.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Remove globin mRNA and ribosomal RNA (rRNA) in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small sample quantities – only 1 μg of total RNA required

Blood is an important sample for research into thousands of rare diseases and other disorders. The sequencing data from samples treated with Globin-Zero are focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms. Globin-Zero removes unwanted globin mRNA, as well as cytoplasmic and mitochondrial rRNA, from mammalian blood RNA samples before sequencing library construction.

Alternative kits

ScriptSeq Complete Gold Kit (Blood): Recommended for sequencing low input samples (e.g. 100 ng of total RNA)

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs

Globin-Zero Gold removes globin mRNA, as well as rRNA from nuclear and mitochondrial regions:

Globin-Zero Gold depletes unwanted globin mRNA and ribosomal RNA from total RNA extracted from blood. Removal of these unwanted subunits produces sequencing results that are heavily focused on BIOLOGically informative genomic regions.

Globin-Zero Gold efficiently removes both globin mRNA and rRNA:

Removal of these unwanted subunits allows generation of sequencing libraries that produce data heavily focused on BIOLOGically informative genetic regions."

"Illumina/Ribo-Zero Gold rRNA Removal Kit (Yeast) 24 reactions/MRZY1324/1 Ea

" MRZY1324 Illumina 产品编号： MRZY1324美  元  价： $2016.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Depletes ribosomal RNA (rRNA)

Sequencing results contain entire transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small samples – only 1 μg of total RNA required

The yeast transcriptome is more complex than previously thought. RNA-Seq of yeast is a valuable approach for mapping the transcriptome to characterize novel and low abundance transcripts. The Ribo-Zero Gold rRNA Removal Kit (Yeast) offers informative sequencing results by removing unwanted rRNA before sequencing.

Gene coverage is enhanced following depletion by Ribo-Zero Gold (Yeast). Subsequent library construction and sequencing produces reads focused on informative genomic regions."

"Illumina/Ribo-Zero rRNA Removal Kit (Plant Leaf) 24 reactions 99% of nuclear-encoded and chloroplast rRNA from 1-5 µg of

" MRZPL1224 Illumina 产品编号： MRZPL1224美  元  价： $2066.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Removes cytoplasmic, mitochondrial, and chloroplast ribosomal RNA (rRNA) in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small sample quantities – only 1 μg of total RNA required

Virtually all rRNA is depleted from intact and partially degraded samples in 2 hours.

Sequencing is an important discovery tool for plant research. The sequencing data from samples treated with Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) offer informative sequencing results by removing unwanted cytoplasmic, mitochondrial, and chloroplast rRNA prior to sequencing. In order to achieve maximum depletion of rRNA, the Ribo-Zero (Plant Leaf) kit contains a higher percentage of probes to deplete chloroplast rRNA compared to Ribo-Zero (Plant Seed/Root).

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kits

ScriptSeq Complete Kit (Plant): Recommended for low input plant samples (e.g. 100 ng of total RNA).

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs"

"Illumina/rRNA Removal Kit (Epidemiology) 24 reactions/MRZE724/1 Ea

" MRZE724 Illumina 产品编号： MRZE724美  元  价： $2066.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Removes cytoplasmic and mitochondrial ribosomal RNA (rRNA) from samples composed of human/mouse/rat, Gram-positive and Gram-negative bacterial RNA (see table)

Requires 500 ng to 2.5 μg total RNA per sample

rRNA removal from both intact and partially degraded RNA

Single-pass, 90- to 120-minute process

Recovers all fragments of human and bacterial non-ribosomal RNAs

Applicable to a broad spectrum of bacteria

The Ribo-Zero Gold rRNA Removal Kit (Epidemiology) combines Ribo-Zero Gold (Human/Mouse/Rat) and Ribo-Zero (Bacteria) rRNA removal reagents into a single solution that effectively removes rRNA, including human mitochondrial RNA, from samples composed of both human/mouse/rat and bacterial RNA.

Hypothesis-free NGS is more sensitive to diversity than 16S profiling

Ribo-Zero Gold rRNA Removal Kit (Epidemiology) has increased sensitivity to BIOLOGical diversity because it captures all metatranscriptomic information (instead of directed capture with 16S profiling).

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Related Information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs

Specifications:

Technology Sequencing

Species Category Human,Mouse,Rat,Bacteria

Specialized Sample Types FFPE

System Compatibility Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000"

"Illumina/Ribo-Zero rRNA Removal Kit (H/M/R) 6 reactions/MRZH116/1 Ea

" MRZH116 Illumina 产品编号： MRZH116美  元  价： $582.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Ribo-Zero rRNA Removal Kit (Human "Product Highlights:

Removes ribosomal RNA (rRNA) in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small samples – only 1 μg of total RNA required

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with Ribo-Zero (Human/Mouse/Rat) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants and isoforms.

Ribo-Zero (Human/Mouse/Rat) offers informative sequencing results by removing unwanted rRNA prior to sequencing. This kit removes cytoplasmic (nuclear-encoded) rRNAs.

Ribo-Zero retains the complete transcriptome

Ribo-Zero removes rRNA in order to retain the regulatory regions of noncoding RNA, even from low-quality samples. Other methods, such as Poly(A) depletion, do not retain noncoding, regulatory information.

Overview of the Ribo-Zero process

Virtually all rRNA is depleted from intact, degraded, and badly degraded (FFPE) samples in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kit versions

The Ribo-Zero Gold (Human/Mouse/Rat) rRNA Removal Kit removes both cytoplasmic and mitochondrial rRNAs, and is compatIBLe with low input samples (e.g. 100 ng of total RNA).

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs"

"Illumina/Ribo-Zero Gold rRNA Removal Kit (H/M/R) (24 reactions) 99% of cytoplasmic and mitochondrial rRNA from human, mo

" MRZG12324 Illumina 产品编号： MRZG12324美  元  价： $2066.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Ribo-Zero Gold rRNA Removal Kit (Human "Product Highlights:

Removes ribosomal RNA (rRNA) in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small sample quantities – only 1 μg of total RNA required

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with Ribo-Zero Gold (Human/Mouse/Rat) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

Ribo-Zero Gold (Human/Mouse/Rat) offers informative sequencing results by removing unwanted rRNA prior to sequencing. This kit removes both cytoplasmic (nuclear-encoded) and mitochondrial rRNAs.

Ribo-Zero retains the complete transcriptome

Ribo-Zero removes rRNA in order to retain the regulatory regions of noncoding RNA, even from low-quality samples. Other methods, such as Poly(A) depletion, do not retain noncoding, regulatory information.

Overview of the Ribo-Zero process

Virtually all rRNA is depleted from intact, degraded, and badly degraded (FFPE) samples in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kits

Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat): Removes cytoplasmic rRNA only.

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs"

"Illumina/Ribo-Zero rRNA Removal Kit (Plant Seed/Root) 6 reactions/MRZSR116/1 Ea

" MRZSR116 Illumina 产品编号： MRZSR116美  元  价： $582.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Removes cytoplasmic, mitochondrial, and chloroplast ribosomal RNA (rRNA) in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small sample quantities – only 1 μg of total RNA required

Virtually all rRNA is depleted from intact and partially degraded samples in 2 hours.

Sequencing is an important discovery tool for plant research. The sequencing data from samples treated with Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) offer informative sequencing results by removing unwanted cytoplasmic, mitochondrial, and chloroplast rRNA prior to sequencing. In order to achieve maximum depletion of rRNA, the Ribo-Zero (Plant Leaf) kit contains a higher percentage of probes to deplete chloroplast rRNA compared to Ribo-Zero (Plant Seed/Root).

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kits

ScriptSeq Complete Kit (Plant): Recommended for low input plant samples (e.g. 100 ng of total RNA).

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs"

"Illumina/Ribo-Zero rRNA Removal Kit (H/M/R) 24 reactions/MRZH11124/1 Ea

" MRZH11124 Illumina 产品编号： MRZH11124美  元  价： $2066.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Ribo-Zero rRNA Removal Kit (Human "Product Highlights:

Removes ribosomal RNA (rRNA) in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small samples – only 1 μg of total RNA required

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with Ribo-Zero (Human/Mouse/Rat) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants and isoforms.

Ribo-Zero (Human/Mouse/Rat) offers informative sequencing results by removing unwanted rRNA prior to sequencing. This kit removes cytoplasmic (nuclear-encoded) rRNAs.

Ribo-Zero retains the complete transcriptome

Ribo-Zero removes rRNA in order to retain the regulatory regions of noncoding RNA, even from low-quality samples. Other methods, such as Poly(A) depletion, do not retain noncoding, regulatory information.

Overview of the Ribo-Zero process

Virtually all rRNA is depleted from intact, degraded, and badly degraded (FFPE) samples in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kit versions

The Ribo-Zero Gold (Human/Mouse/Rat) rRNA Removal Kit removes both cytoplasmic and mitochondrial rRNAs, and is compatIBLe with low input samples (e.g. 100 ng of total RNA).

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs"

"Illumina/Ribo-Zero Gold rRNA Removal Kit (H/M/R) (6 reactions)/MRZG126/1 Ea

" MRZG126 Illumina 产品编号： MRZG126美  元  价： $582.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Ribo-Zero Gold rRNA Removal Kit (Human "Product Highlights:

Removes ribosomal RNA (rRNA) in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small sample quantities – only 1 μg of total RNA required

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with Ribo-Zero Gold (Human/Mouse/Rat) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

Ribo-Zero Gold (Human/Mouse/Rat) offers informative sequencing results by removing unwanted rRNA prior to sequencing. This kit removes both cytoplasmic (nuclear-encoded) and mitochondrial rRNAs.

Ribo-Zero retains the complete transcriptome

Ribo-Zero removes rRNA in order to retain the regulatory regions of noncoding RNA, even from low-quality samples. Other methods, such as Poly(A) depletion, do not retain noncoding, regulatory information.

Overview of the Ribo-Zero process

Virtually all rRNA is depleted from intact, degraded, and badly degraded (FFPE) samples in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kits

Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat): Removes cytoplasmic rRNA only.

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs

Specifications:

Technology Sequencing

Specialized Sample Types FFPE

Species Category Human,Mouse,Rat

System Compatibility MiSeq,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000"

"Illumina/Ribo-Zero rRNA Removal Kit (Bacteria) 6 reactions/MRZMB126/1 Ea

" MRZMB126 Illumina 产品编号： MRZMB126美  元  价： $582.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Removes ribosomal RNA (rRNA) from intact and partially degraded mixtures of Gram (+) and Gram (–) bacteria in a single pass

Sequencing data contains complete transcriptome of coding and noncoding RNA species

Good for small sample quantities – only 1 μg of total RNA required

Virtually all rRNA is depleted from intact and partially degraded samples in 2 hours.

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with the Ribo-Zero rRNA Removal Kit (Bacteria) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants and isoforms.

This kit offers informative sequencing results by removing unwanted cytoplasmic (nuclear-encoded) rRNA from bacterial mixtures prior to sequencing.

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads

Step 2: Treat samples with rRNA Removal Solution

Step 3: Remove rRNA

Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kits

ScriptSeq Complete Kit (Bacteria): Recommended for sequencing low input samples (e.g. 100 ng of total RNA).

Related information

rRNA Removal Kit Selection Guide

Ribo-Zero Kit Species Compatibility

Ribo-Zero Kit FAQs"

"Illumina/Ribo-Zero Gold rRNA Removal Kit (Yeast) 6 reactions/MRZY1306/1 Ea

" MRZY1306 Illumina 产品编号： MRZY1306美  元  价： $567.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>核酸提取纯化>RNA提取纯化试剂公司分类： Molecular Biology Reagents "Product Highlights:

Depletes ribosomal RNA (rRNA)

Sequencing results contain entire transcriptome of coding and noncoding RNA species

Good for intact and partially degraded small samples – only 1 μg of total RNA required

The yeast transcriptome is more complex than previously thought. RNA-Seq of yeast is a valuable approach for mapping the transcriptome to characterize novel and low abundance transcripts. The Ribo-Zero Gold rRNA Removal Kit (Yeast) offers informative sequencing results by removing unwanted rRNA before sequencing.

Gene coverage is enhanced following depletion by Ribo-Zero Gold (Yeast). Subsequent library construction and sequencing produces reads focused on informative genomic regions."

Illumina

"Illumina/Tip Depth Gauge/A1-99-102/1 Ea

" A1-99-102 Illumina 产品编号： A1-99-102美  元  价： $150.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

The Tip Depth Gauge calibrates the probe depth for the Tecan Genesis Freedom EVO or Freedom EVO-2 liquid-handling robot used during automation of array-based assays such as the Infinium Assay."

"Illumina/Infinium Te-Flow Thermometer Assembly/A1-99-109/1 Ea

" A1-99-109 Illumina 产品编号： A1-99-109美  元  价： $680.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

The Infinium TeFlow Thermometer Assembly, which includes the Thermometer and a temperature probe, reads the temperature in the TeFlow rack during Infinium Whole-Genome Genotyping Assays and Infinium HD Assays."

"Illumina/Infinium Multi-Sample Alignment Fixture (1)/WG-15-310/1 Ea

" WG-15-310 Illumina 产品编号： WG-15-310美  元  价： $650.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

The Infinium Alignment Fixture allows correct assembly of the TeFlow Chamber, HD or LCG BeadChip array, and spacer to ensure optimal processing during the Infinium Assay."

Illumina

"Illumina/cBot 2 Barcoded Strip Tubes (8 well)/20005160/1 Ea

" 20005160 Illumina 产品编号： 20005160美  元  价： $500.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

The cBot 2 barcoded 8-well strip tube enables sample tracking though the sequencing workflow. The keyed strip tube minimizes sample mix-ups by single orientation loADIng and a unique barcode that is read by an onboard barcode scanner on the cBot 2 System.

Each strip tube has two barcodes enabling sample tracking on liquid handling robots and the cBot 2 System. Each strip tube box contains 100 strip tubes.

This item is an accessory for the cBot 2 System, which automates cluster generation on HiSeq sequencing systems.

Specifications:

Technology Sequencing

System Compatibility HiSeq 4000,HiSeq X Ten,HiScanSQ,HiSeq 3000,HiSeq X Five,HiSeq 2500"

"Illumina/Infinium MIDI Heatblock Insert/BD-60-601/1 Ea

" BD-60-601 Illumina 产品编号： BD-60-601美  元  价： $160.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products

"Illumina/Infinium BeadChip Glass Tray (1)/BD-60-460/1 Ea

" BD-60-460 Illumina 产品编号： BD-60-460美  元  价： $62.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

The BeadChip Wash Rack and Glass Tray (purchased separately) are used to handle BeadChip arrays following hybridization during Direct Hybridization and Infinium Assays."

"Illumina/Infinium Hybridization Chamber Inserts (8)/WG-15-301/1 Ea

" WG-15-301 Illumina 产品编号： WG-15-301美  元  价： $1200.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products

"Illumina/Infinium Hybridization Chamber Inserts (8)/WG-15-301/1 Ea

" WG-15-301 Illumina 产品编号： WG-15-301美  元  价： $1200.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

The Infinium Hybridization Chambers are used for BeadChip array hybridization during Infinium Assays. Each Infinium Hybridization Chamber processes four Infinium BeadChip arrays per day.

A gasket fits inside the chamber to ensure proper BeadChip retention and optimal exposure to reagents. One gasket is included in the package, and replacement gaskets are available for purchase separately."

"Illumina/Replacement Infinium Hybridization Chamber Gasket (1)/BD-60-403/1 Ea

" BD-60-403 Illumina 产品编号： BD-60-403美  元  价： $7.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

The Infinium Hybridization Chambers are used for BeadChip array hybridization during Infinium Assays. Each Infinium Hybridization Chamber processes four Infinium BeadChip arrays per day.

A gasket fits inside the chamber to ensure proper BeadChip retention and optimal exposure to reagents. One gasket is included in the package, and replacement gaskets are available for purchase separately."

"Illumina/Infinium Hybridization Chamber (1)/BD-60-402/1 Ea

" BD-60-402 Illumina 产品编号： BD-60-402美  元  价： $840.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

The Infinium Hybridization Chambers are used for BeadChip array hybridization during Infinium Assays. Each Infinium Hybridization Chamber processes four Infinium BeadChip arrays per day.

A gasket fits inside the chamber to ensure proper BeadChip retention and optimal exposure to reagents. One gasket is included in the package, and replacement gaskets are available for purchase separately."

"Illumina/Infinium BeadChip Boxes (10)/BD-60-500/1 Ea

" BD-60-500 Illumina 产品编号： BD-60-500美  元  价： $40.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

Store BeadChips used in Illumina array-based assays. Each BeadChip storage box holds 25 BeadChips."

"Illumina/Infinium BeadChip Expansion Package/WG-10-201/1 Ea

" WG-10-201 Illumina 产品编号： WG-10-201美  元  价： $7000.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

Store BeadChips used in Illumina array-based assays. Each BeadChip storage box holds 25 BeadChips."

"Illumina/Teflon-coated replacement probes for Tecan Robot (1)/A1-99-101/1 Ea

" A1-99-101 Illumina 产品编号： A1-99-101美  元  价： $70.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>配件>常用设备公司分类： Accessory Products "Product Highlights:

Store BeadChips used in Illumina array-based assays. Each BeadChip storage box holds 25 BeadChips."

Illumina

"Illumina/MiSeqDx Cystic Fibrosis 139-Variant Assay (20 runs, 960 samples)/DX-102-1003/1 Ea

" DX-102-1003 Illumina 产品编号： DX-102-1003美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>临床研究公司分类： In Vitro Diagnostic (IVD) Products "Product Highlights:

Compared to the current ACMG recommended cystic fibrosis variant panel, the MiSeqDx Cystic Fibrosis 139-Variant Assay offers:

Improved Detection Rates of Cystic Fibrosis-Causing Variants

Detection of couples at risk increased from 72% to ~91%1

Reduced Additional Testing

Highly accurate and reproducIBLe sequencing technology delivers dependable results

The MiSeqDx Cystic Fibrosis 139-Variant Assay is an FDA-cleared in vitro diagnostic (IVD) next-generation sequencing test designed to detect 139 CFTR variants as defined in the CFTR2 database. Other methods test only for those variants most commonly found in Caucasians, potentially missing CF-causing variants across broader ethnic groups. The MiSeqDx Cystic Fibrosis 139-Variant Assay offers the largest CF variant panel to overcome this bias and provide comprehensive detection across diverse demographics.2

Widely adopted next-generation sequencing

The MiSeqDx Cystic Fibrosis 139-Variant Assay provides a fully integrated molecular CF testing solution on the MiSeqDx instrument. Simply prepare libraries from 250 ng gDNA, load on to the MiSeqDx for sequencing using Illumina sequencing by synthesis (SBS) chemistry, and analyze with the user-friendly software. The automated workflow is quick and easy to perform, potentially minimizing human error.

A complete kit for library preparation and sequencing

The MiSeqDx Cystic Fibrosis 139-Variant Assay includes all index primers and reagents needed for library preparation, sample multiplexing, and sequencing in a single kit. MiSeqDx sequencing reagents are packaged in a convenient ready-to-use format, minimizing hands-on time and increasing uniformity in all tests. To accommodate a broad spectrum of testing needs, from small to large volumes, the assay is available in two kit configurations (2 or 20 runs).

Specifications:

Assay Time 2.5 days

Hands-On Time 3.5 hours

Input Quantity 250 ng genomic DNA

System Compatibility MiSeqDx

Technology Sequencing"

"Illumina/MiSeqDx Universal Kit (2 runs, up to 96 samples)/DX-103-1001/1 Ea

" DX-103-1001 Illumina 产品编号： DX-103-1001美  元  价： $12410.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>临床研究公司分类： In Vitro Diagnostic (IVD) Products "Product Highlights:

FlexIBLe Assay Design - Develop assays to meet the specific needs of your customers

Validated Kit – Verified performance standards for supplied library preparation and sequencing reagents

Fast Results - Go from genomic DNA to fully analyzed data in < 2 days

High Assay Reproducibility - Consistent results between multiple operators and systems

The MiSeqDx Universal Kit is a validated, FDA-cleared amplicon sequencing solution that enables clinical laboratories to develop their own next-generation sequencing (NGS) assays for use on the FDA-cleared MiSeqDx instrument. Together, the MiSeqDx Universal Kit and instrument offer molecular diagnostics labs the accuracy and reliABIlity needed to bring NGS capABIlities into their facilities.

Customized assay design

With the MiSeqDx Universal Kit, molecular diagnostics labs develop assays using oligonucleotide probes they have designed on their own. This provides users with the flexibility to target specific regions of interest and better meet the needs of their customers.

Streamlined workflow

Prepare libraries, load on to the MiSeqDx for sequencing using the Illumina sequencing by synthesis (SBS) chemistry, and analyze with the user-friendly software. The automated workflow is quick and easy to perform, potentially minimizing human error.

Specifications:

Assay Time 1 day

Hands-On Time 2.5 hours

Input Quantity 250 ng genomic DNA

System Compatibility MiSeqDx

Technology Sequencing"

"Illumina/MiSeqDx Cystic Fibrosis Clinical Sequencing Assay (6 runs, 48 samples)/DX-102-1001/1 Ea

" DX-102-1001 Illumina 产品编号： DX-102-1001美  元  价： $9504.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>临床研究公司分类： In Vitro Diagnostic (IVD) Products "Product Highlights:

Complete View of the CFTR Gene

Capture all variants in the protein coding regions and intron/exon boundaries of the CFTR gene

Accurate Results

Deep coverage (> 3,000×) allows detection accuracy with a Positive Agreement (PA) of 99.66%*

No Demographic Bias

Sequencing the CFTR gene removes the bias inherent in existing genotyping panels1

* PA includes polyTG/polyT variants

The MiSeqDx Cystic Fibrosis Clinical Sequencing Assay is the first FDA-cleared in vitro diagnostic (IVD) next-generation sequencing (NGS) test designed to sequence all protein coding regions and intron/exon boundaries of the CFTR gene, including two large deletions, two deep intronic mutations, and indels in homopolymeric regions such as the 2184delA deletion. The assay also automatically detects polyTG/polyT variants. Viewing the CFTR sequence eliminates any bias inherent in existing cystic fibrosis (CF) panels. As a result, the MiSeqDx Cystic Fibrosis Clinical Sequencing Assay can uncover rare mutations not accounted for in standard tests.

Widely adopted next-generation sequencing

The MiSeqDx Cystic Fibrosis Clinical Sequencing Assay provides a fully integrated molecular CF testing solution on the MiSeqDx instrument. Amplify 27 CFTR gene target regions in a single PCR step, and multiplex samples to streamline your workflow. Simply prepare libraries from 250 ng gDNA, load on to the MiSeqDx for sequencing using the Illumina sequencing by synthesis (SBS) chemistry, and analyze with the user-friendly software.

A complete kit for library preparation and sequencing

The MiSeqDx Cystic Fibrosis Clinical Sequencing Assay includes all index primers and reagents needed for library preparation, sample multiplexing, and sequencing in a single kit. MiSeqDx sequencing reagents are packaged in a convenient ready-to-use format, minimizing hands-on time, and increasing uniformity in all tests.

Specifications:

Assay Time 2.5 days

Hands-On Time 3.5 hours

Input Quantity 250 ng genomic DNA

System Compatibility MiSeqDx

Technology Sequencing"

"Illumina/MiSeqDx Cystic Fibrosis 139-Variant Assay (2 runs, 96 samples)/DX-102-1004/1 Ea

" DX-102-1004 Illumina 产品编号： DX-102-1004美  元  价： $5760.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>临床研究公司分类： In Vitro Diagnostic (IVD) Products "Product Highlights:

Compared to the current ACMG recommended cystic fibrosis variant panel, the MiSeqDx Cystic Fibrosis 139-Variant Assay offers:

Improved Detection Rates of Cystic Fibrosis-Causing Variants

Detection of couples at risk increased from 72% to ~91%1

Reduced Additional Testing

Highly accurate and reproducIBLe sequencing technology delivers dependable results

The MiSeqDx Cystic Fibrosis 139-Variant Assay is an FDA-cleared in vitro diagnostic (IVD) next-generation sequencing test designed to detect 139 CFTR variants as defined in the CFTR2 database. Other methods test only for those variants most commonly found in Caucasians, potentially missing CF-causing variants across broader ethnic groups. The MiSeqDx Cystic Fibrosis 139-Variant Assay offers the largest CF variant panel to overcome this bias and provide comprehensive detection across diverse demographics.2

Widely adopted next-generation sequencing

The MiSeqDx Cystic Fibrosis 139-Variant Assay provides a fully integrated molecular CF testing solution on the MiSeqDx instrument. Simply prepare libraries from 250 ng gDNA, load on to the MiSeqDx for sequencing using Illumina sequencing by synthesis (SBS) chemistry, and analyze with the user-friendly software. The automated workflow is quick and easy to perform, potentially minimizing human error.

A complete kit for library preparation and sequencing

The MiSeqDx Cystic Fibrosis 139-Variant Assay includes all index primers and reagents needed for library preparation, sample multiplexing, and sequencing in a single kit. MiSeqDx sequencing reagents are packaged in a convenient ready-to-use format, minimizing hands-on time and increasing uniformity in all tests. To accommodate a broad spectrum of testing needs, from small to large volumes, the assay is available in two kit configurations (2 or 20 runs).

Specifications:

Assay Time 2.5 days

Hands-On Time 3.5 hours

Input Quantity 250 ng genomic DNA

System Compatibility MiSeqDx

Technology Sequencing

"

Illumina

Illumina/TruSight RNA Fusion Panel Set A (48 samples)/RS-304-1002/1 Ea RS-304-1002 Illumina 产品编号： RS-304-1002美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

This targeted RNA sequencing panel is a cost-effective solution to detect gene fusions in multiple cancer types, regardless of origin. Covering 507 fusion-associated genes, a single assay enables researchers to assess most known cancer-related fusions in blood, bone marrow, and FFPE samples, with the power to identify novel fusion gene partners.

Industry-validated content for a comprehensive view of cancer-related fusion genes, detecting common and novel fusions

From RNA to results in 4 days including on-instrument software that displays fusion calls

Compatibility with desktop sequencers to maximize lab budgets

Optimized RNA sequencing for all sample types, including FFPE tissues

Simple Fusion Report for Customers New to NGS

On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman database), and evidence of fusion-supporting reads.

Robust, ReproducIBLe, and Low-Input Fusion Assay

This assay accommodates as little input as 20 ng FFPE RNA or 10 ng fresh-frozen total RNA. The TruSight RNA Fusion Panel provides a sensitive, reproducIBLe, and economical solution for studies of gene fusions in cancer research.

Specifications:

Assay Time 2.5 days

Hands-On Time 11 hours

Input Quantity 10 ng total RNA, 20–100 ng FFPE RNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Method Targeted RNA Sequencing,Sequencing for Cytogenomics

Variant Class Gene Fusions

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Species Category Human

Cancer Type Pan-Cancer"

"Illumina/TruSight Rapid Capture (4 indexes, 16 samples, 4 enrichments)/FC-140-1103/1 Ea

" FC-140-1103 Illumina 产品编号： FC-140-1103美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London

Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets

6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

* Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 1.5 days

Input Quantity 50 ng

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Variant Class Germline Variants

Cancer Type Pan-Cancer

Method Targeted DNA Sequencing"

"Illumina/TruSight HLA v2 Sequencing Panel (24 samples, automated)/20005170/1 Ea

" 20005170 Illumina 产品编号： 20005170美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:

High-resolution sequencing of 11 HLA loci

Workflow with library preparation, sequencing and data analysis/reporting

Accurate and high-resolution HLA typing for unambiguous results

Capture Full HLA Gene Sequences

TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.

Sample-to-Report Workflow with Optimized Data Analysis

The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexIBLe options for post-analysis reporting of results.

High-Accuracy HLA Typing

The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.

How TruSight HLA v2 Works

TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.

Analysis Software Highlights

The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.

Specifications:

Assay Time 48 hours

Hands-On Time 4.5 hours

Input Quantity 400 ng DNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Variant Class Single Nucleotide Polymorphisms (SNPs)

Technology Sequencing

Species Category Human

Method HLA Sequencing"

Illumina/TruSight Tumor 170 Kit plus Watson for Genomics/20018622/1 Ea 20018622 Illumina 产品编号： 20018622美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight Tumor 170, a next-generation sequencing assay designed to cover 170 genes associated with common solid tumors, is an enrichment-based targeted panel that simultaneously analyzes DNA and RNA, covering a wide range of genes and variant types. The comprehensive nature provides laboratories with a deep view into the genetics of cancer.

Comprehensive Coverage of Cancer-Related Variants

Assessment of fusions, splice variants, insertions/deletions and single-nucleotide variants (SNVs), and amplifications in one assay using DNA and RNA creates efficiencies in sample usage, time, and cost.

Accurate Results from Low-Quality Samples1

Variant detection with as little as 40 ng DNA and RNA input, and as low as 5% mutant allele frequency, maximizes the results from precious formalin-fixed paraffin-embedded (FFPE) samples.

Integrated, Streamlined Workflow

DNA and RNA are prepared in parallel with an integrated workflow following DNA shearing/CDNA synthesis.

Specifications:

Assay Time ~2 days (Library Prep)

Hands-On Time ~10.5 hours

Input Quantity 40 ng DNA and/or RNA

Method Targeted RNA Sequencing,Targeted DNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Somatic Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE,Low Input

System Compatibility NextSeq 550,NextSeq 500,HiSeq 2500

Technology Sequencing

Species Category Human

Cancer Type Solid Tumor

Scientific Posters:

AACR 2017: TruSight Tumor 170 and Tumor Mutational Burden

Results of analysis indicate that TruSight Tumor 170, with comprehensive coverage of cancer-related genes, shows high concordance with whole exome sequencing for accurate assessment of tumor mutational burden.

Learn More

AACR 2017: TruSight Tumor 170 and Solid Tumor Profiling Analytical Performance with FFPE Samples

Standard extractions from FFPE embedded samples provide sufficient material (40ng) in >95% of samples that were extracted by Illumina. This data shows that the TruSight Tumor 170 panel is a robust assay that generates passing sample QC data in >85% of samples with varying quality, and in >95% of samples that have quality metrics that fall within the recommendations for the kit.

Learn More

AACR 2017: TruSight Tumor 170 for Small Nucleotide Variations and Gene Amplifications in FFPE DNA Samples

TruSight Tumor 170 can achieve high sensitivity and specificity for the detection of somatic variants (small variants and CNVs) from DNA extracted from FFPE tissues.

Learn More

AACR 2017: TruSight Tumor 170 for Fusions and Splice Variants in FFPE RNA Tumor Samples

Through examining limit of detection in the context of RNA expression, this study shows that TruSight Tumor 170 provides high sensitivity and specificity in RNA variant calling down to 5 copies of transcript per ng of input.

Learn More"

"Illumina/TruSight Rapid Capture (24 indexes, 48 samples, 4 enrichments)/FC-140-1104/1 Ea

" FC-140-1104 Illumina 产品编号： FC-140-1104美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London

Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets

6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

* Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 1.5 days

Input Quantity 50 ng

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Variant Class Germline Variants

Cancer Type Pan-Cancer

Method Targeted DNA Sequencing"

"Illumina/TruSight One Expanded Sequencing Panel (36 samples)/FC-141-2007/1 Ea

" FC-141-2007 Illumina 产品编号： FC-141-2007美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight One Sequencing Panels enable labs to expand and streamline their sequencing portfolio, while managing costs.

Two panels offer options to target up to 6700 genes associated with human disease, with high coverage

Single assay includes multiple genes of interest, replacing iterative testing

Easy, flexIBLe gene filtering and annotation options simplify BIOLOGical interpretation and reporting

These panels provide clinical research labs with an affordable solution for managing a diverse assay portfolio. The TruSight One Sequencing Panel provides comprehensive coverage of > 4800 disease-associated genes, while the TruSight One Expanded Sequencing Panel targets ~1900 additional genes with recent disease associations in the scientific literature. Investigators can choose to analyze all of the genes on the panel or focus on a specific subset. With a single assay, labs can expand existing menus, streamline workflows, or create an entire portfolio of sequencing options.

Specifications:

Input Quantity 50 ng

Content Specifications ~12 Mb genomic content for TruSight One; ~16.5 Mb genomic content for TruSight One Expanded

Multiplexing Up to 96-plex

System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Method Targeted DNA Sequencing"

"Illumina/TruSight Rapid Capture (24 indexes, 96 samples, 8 enrichments)/FC-140-1105/1 Ea

" FC-140-1105 Illumina 产品编号： FC-140-1105美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London

Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets

6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

* Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 1.5 days

Input Quantity 50 ng

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Variant Class Germline Variants

Cancer Type Pan-Cancer

Method Targeted DNA Sequencing"

"Illumina/TruSight RNA Pan-Cancer Panel Set B/RS-303-1003/1 Ea

" RS-303-1003 Illumina 产品编号： RS-303-1003美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The TruSight RNA Pan-Cancer panel provides a comprehensive analysis of the cancer transcriptome. It offers:

Gene expression information, variant calling, and fusion detection with known and novel gene fusion partners

Optimized, low-input protocol for a wide range of sample types including FFPE

A comprehensive view of cancer pathways

Economical RNA sequencing (RNA-Seq) on a desktop sequencer

Highly Sensitive and Economical Targeted Sequencing

The TruSight RNA Pan-Cancer panel enables the quantitative measurement of gene expression as well as the detection of gene fusions with both known and novel gene fusion partners. The panel accommodates as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity at 8 samples per run on a desktop sequencer, allowing cost-effective access to NGS for any lab.

Intuitive Cloud-based Data Analysis

Analysis can be performed using the RNA-Seq Alignment BaseSpace App. This intuitive tool performs fusion calling andvariant detection, and provides gene expression profiles, offering a comprehensive solution to all cancer researchers.

TruSight RNA Pan-Cancer Sample Datasets

MiSeq Data

10 ng of human reference RNA (UHR), human reference brain RNA (Brain), cell line RNA (MCF7), and 20 ng of breast tumor FFPE RNA (BT) were prepared using the TruSight RNA Pan-Cancer Panel and sequenced on the MiSeq System. Read mapping and fusion calling were performed using the RNA-Seq Alignment App with STAR aligner on BaseSpace Sequence Hub.

View Project

MiniSeq Data

Human reference RNA (HBRR and UHRR), cell line RNA (MCF7), and breast tumor RNA samples were prepared using the TruSight RNA Pan-Cancer panel, and 8 samples were sequenced on the MiniSeq System at a 2x76bp read length with single indexing. The total yield was 4.2 Gb with 96.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

Access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 2.5 days

Hands-On Time 11 hours

Input Quantity 10 ng total RNA,20 - 100 ng FFPE RNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Method Targeted RNA Sequencing,Sequencing for Cytogenomics

Variant Class Gene Fusions,Somatic Variants,Novel Transcripts,Transcript Variants

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Species Category Human

Cancer Type Pan-Cancer

"

"Illumina/TruSight HLA v2 Sequencing Panel (24 samples)/20000215/1 Ea

" 20000215 Illumina 产品编号： 20000215美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:

High-resolution sequencing of 11 HLA loci

Workflow with library preparation, sequencing and data analysis/reporting

Accurate and high-resolution HLA typing for unambiguous results

Capture Full HLA Gene Sequences

TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.

Sample-to-Report Workflow with Optimized Data Analysis

The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexIBLe options for post-analysis reporting of results.

High-Accuracy HLA Typing

The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.

How TruSight HLA v2 Works

TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.

Analysis Software Highlights

The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.

Specifications:

Assay Time 48 hours

Hands-On Time 4.5 hours

Input Quantity 400 ng DNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Variant Class Single Nucleotide Polymorphisms (SNPs)

Technology Sequencing

Species Category Human

Method HLA Sequencing"

"Illumina/TruSight Tumor 15 MiniSeq Kit/20005610/1 Ea

" 20005610 Illumina 产品编号： 20005610美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight Tumor 15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly mutated in solid tumors. It accurately detects low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed, paraffin-embedded (FFPE) tumor tissue.

View the Gene List

Featuring a rapid workflow that can be easily integrated into lab procedures, this panel offers a single assay for accurate, economical, and rapid analysis of solid tumors.

This TruSight Tumor panel offers:

Comprehensive workflow: Assess 15 genes with one simple workflow instead of single, iterative gene testing with polymerase chain reaction (PCR)

Efficient: Rapid turnaround with only 3.5 hours of hands-on time, going from DNA to data in approximately 36 hours

Relevant gene content for solid tumors: Somatic variants selected from relevant industry guidelines1,2, key opinion leaders3,4, and pharmaceutical research

Sensitive variant detection from low DNA input: Accurate somatic variant detection of 5% allele frequency using 20 ng DNA from FFPE tissue samples

TruSight Tumor 15 Sample Datasets

3 human reference samples and 5 FFPE-exacted DNA samples from lung, colon, melanoma, and breast tumors were prepared using TruSight Tumor 15. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 150 bp read length configuration with dual indexing. The total yield was 7.6 Gb with 94.9% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

Access to this data requires a BaseSpace Sequence Hub login. 

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 7 hours

Hands-On Time 3.5 hours

Input Quantity 20 ng

System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Variant Class Somatic Variants,Insertions-Deletions (indels)

Species Category Human

Cancer Type Solid Tumor

Method Amplicon Sequencing,Targeted DNA Sequencing"

"Illumina/TruSight RNA Pan-Cancer Set A MiniSeq Kit/20005611/1 Ea

" 20005611 Illumina 产品编号： 20005611美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The TruSight RNA Pan-Cancer panel provides a comprehensive analysis of the cancer transcriptome. It offers:

Gene expression information, variant calling, and fusion detection with known and novel gene fusion partners

Optimized, low-input protocol for a wide range of sample types including FFPE

A comprehensive view of cancer pathways

Economical RNA sequencing (RNA-Seq) on a desktop sequencer

Highly Sensitive and Economical Targeted Sequencing

The TruSight RNA Pan-Cancer panel enables the quantitative measurement of gene expression as well as the detection of gene fusions with both known and novel gene fusion partners. The panel accommodates as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity at 8 samples per run on a desktop sequencer, allowing cost-effective access to NGS for any lab.

Intuitive Cloud-based Data Analysis

Analysis can be performed using the RNA-Seq Alignment BaseSpace App. This intuitive tool performs fusion calling andvariant detection, and provides gene expression profiles, offering a comprehensive solution to all cancer researchers.

TruSight RNA Pan-Cancer Sample Datasets

MiSeq Data

10 ng of human reference RNA (UHR), human reference brain RNA (Brain), cell line RNA (MCF7), and 20 ng of breast tumor FFPE RNA (BT) were prepared using the TruSight RNA Pan-Cancer Panel and sequenced on the MiSeq System. Read mapping and fusion calling were performed using the RNA-Seq Alignment App with STAR aligner on BaseSpace Sequence Hub.

View Project

MiniSeq Data

Human reference RNA (HBRR and UHRR), cell line RNA (MCF7), and breast tumor RNA samples were prepared using the TruSight RNA Pan-Cancer panel, and 8 samples were sequenced on the MiniSeq System at a 2x76bp read length with single indexing. The total yield was 4.2 Gb with 96.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

Access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 2.5 days

Hands-On Time 11 hours

Input Quantity 10 ng total RNA,20 - 100 ng FFPE RNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Method Targeted RNA Sequencing,Sequencing for Cytogenomics

Variant Class Gene Fusions,Somatic Variants,Novel Transcripts,Transcript Variants

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Species Category Human

Cancer Type Pan-Cancer

"

"Illumina/TG TruSight Inherited Disease Sequencing Panel (48 Samples)/TG-141-1005/1 Ea

" TG-141-1005 Illumina 产品编号： TG-141-1005美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Developed in collaboration with Dr. Stephen Kingsmore and team during his tenure at Children's Mercy Hospital (CMH) for Pediatric Genomic Medicine, Dr. Carol Saunders at CMH, and Dr. Hilger Ropers at the Max Planck Institute

Targets 552 genes, including coding exons, intron-exon boundaries, and regions known to harbor pathogenic mutations

TruSight Inherited Disease was initially based on a 448 disease panel designed for preconception carrier testing for severe, recessive childhood diseases published by Dr. Kingsmore and team in Science Translational Medicine1.

The original content was revised by Dr. Saunders, FACMG, at CMH (following ACMG guidelines for testing ultra-rare genetic diseases) to reflect the needs of medical geneticists with a primary focus on severe recessive diseases with childhood onset. Intellectual disABIlity genes were added by Dr. Ropers.

The TruSight Inherited Disease sequencing panel set includes custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions.

Specifications:

Input Quantity 50 ng DNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Method Targeted DNA Sequencing"

"Illumina/TruSeq Index Plate Fixture Kit (2 fixtures)/FC-130-1005/1 Ea

" FC-130-1005 Illumina 产品编号： FC-130-1005美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:

High-resolution sequencing of 11 HLA loci

Workflow with library preparation, sequencing and data analysis/reporting

Accurate and high-resolution HLA typing for unambiguous results

Capture Full HLA Gene Sequences

TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.

Sample-to-Report Workflow with Optimized Data Analysis

The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexIBLe options for post-analysis reporting of results.

High-Accuracy HLA Typing

The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.

How TruSight HLA v2 Works

TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.

Analysis Software Highlights

The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.

Specifications:

Assay Time 48 hours

Hands-On Time 4.5 hours

Input Quantity 400 ng DNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Variant Class Single Nucleotide Polymorphisms (SNPs)

Technology Sequencing

Species Category Human

Method HLA Sequencing"

"Illumina/TruSight Tumor 170 Kit (24 Samples)/OP-101-1004/1 Ea

" OP-101-100 Illumina 产品编号： OP-101-1004美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight Tumor 170, a next-generation sequencing assay designed to cover 170 genes associated with common solid tumors, is an enrichment-based targeted panel that simultaneously analyzes DNA and RNA, covering a wide range of genes and variant types. The comprehensive nature provides laboratories with a deep view into the genetics of cancer.

Comprehensive Coverage of Cancer-Related Variants

Assessment of fusions, splice variants, insertions/deletions and single-nucleotide variants (SNVs), and amplifications in one assay using DNA and RNA creates efficiencies in sample usage, time, and cost.

Accurate Results from Low-Quality Samples1

Variant detection with as little as 40 ng DNA and RNA input, and as low as 5% mutant allele frequency, maximizes the results from precious formalin-fixed paraffin-embedded (FFPE) samples.

Integrated, Streamlined Workflow

DNA and RNA are prepared in parallel with an integrated workflow following DNA shearing/CDNA synthesis.

Specifications:

Assay Time ~2 days (Library Prep)

Hands-On Time ~10.5 hours

Input Quantity 40 ng DNA and/or RNA

Method Targeted RNA Sequencing,Targeted DNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Somatic Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE,Low Input

System Compatibility NextSeq 550,NextSeq 500,HiSeq 2500

Technology Sequencing

Species Category Human

Cancer Type Solid Tumor

Scientific Posters:

AACR 2017: TruSight Tumor 170 and Tumor Mutational Burden

Results of analysis indicate that TruSight Tumor 170, with comprehensive coverage of cancer-related genes, shows high concordance with whole exome sequencing for accurate assessment of tumor mutational burden.

Learn More

AACR 2017: TruSight Tumor 170 and Solid Tumor Profiling Analytical Performance with FFPE Samples

Standard extractions from FFPE embedded samples provide sufficient material (40ng) in >95% of samples that were extracted by Illumina. This data shows that the TruSight Tumor 170 panel is a robust assay that generates passing sample QC data in >85% of samples with varying quality, and in >95% of samples that have quality metrics that fall within the recommendations for the kit.

Learn More

AACR 2017: TruSight Tumor 170 for Small Nucleotide Variations and Gene Amplifications in FFPE DNA Samples

TruSight Tumor 170 can achieve high sensitivity and specificity for the detection of somatic variants (small variants and CNVs) from DNA extracted from FFPE tissues.

Learn More

AACR 2017: TruSight Tumor 170 for Fusions and Splice Variants in FFPE RNA Tumor Samples

Through examining limit of detection in the context of RNA expression, this study shows that TruSight Tumor 170 provides high sensitivity and specificity in RNA variant calling down to 5 copies of transcript per ng of input.

Learn More"

"Illumina/TruSight Rapid Capture (1 index, 8 samples, 8 enrichments)/FC-140-1101/1 Ea

" FC-140-1101 Illumina 产品编号： FC-140-1101美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London

Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets

6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

* Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 1.5 days

Input Quantity 50 ng

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Variant Class Germline Variants

Cancer Type Pan-Cancer

Method Targeted DNA Sequencing"

"Illumina/TruSight Tumor 26/FC-130-2001/1 Ea

" FC-130-2001 Illumina 产品编号： FC-130-2001美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight Tumor 26 takes a deeper view of variation in solid tumors including lung, colon, melanoma, gastric and ovarian. This enables clinical researchers to identify low-frequency variation across 26 genes for a more comprehensive view of somatic variation.

Highly accurate variant analysis at limit of detection below 5% variant allele frequency across 174 amplicons, with 1000x minimum coverage of each region

Exceptional sample success rates with minimal DNA input for accurate base calling, even in degraded FFPE samples

Coverage of complete exons for analysis of molecular heterogeneity in highly relevant content selected from CAP1 and NCCN2 guidelines, and late stage clinical trials3

This panel provides amplicon-based library preparation reagents, DNA QC, sample indexes, and oligos targeting identified regions of interest.

Genes in TruSight Tumor 26

See which solid tumor-related genes are represented in TruSight Tumor 26. View Gene List

Specifications:

Assay Time 11 hours

Hands-On Time 4 hours

Input Quantity 30 to 300 ng depending on QC results

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Specialized Sample Types FFPE

Technology Sequencing

Variant Class Somatic Variants,Insertions-Deletions (indels)

Species Category Human

Cancer Type Solid Tumor

Method Amplicon Sequencing,Targeted DNA Sequencing"

"Illumina/TruSight Tumor 170 Kit, For Use with NextSeq (24 Samples)/OP-101-1003/1 Ea

" OP-101-1003 Illumina 产品编号： OP-101-1003美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight Tumor 170, a next-generation sequencing assay designed to cover 170 genes associated with common solid tumors, is an enrichment-based targeted panel that simultaneously analyzes DNA and RNA, covering a wide range of genes and variant types. The comprehensive nature provides laboratories with a deep view into the genetics of cancer.

Comprehensive Coverage of Cancer-Related Variants

Assessment of fusions, splice variants, insertions/deletions and single-nucleotide variants (SNVs), and amplifications in one assay using DNA and RNA creates efficiencies in sample usage, time, and cost.

Accurate Results from Low-Quality Samples1

Variant detection with as little as 40 ng DNA and RNA input, and as low as 5% mutant allele frequency, maximizes the results from precious formalin-fixed paraffin-embedded (FFPE) samples.

Integrated, Streamlined Workflow

DNA and RNA are prepared in parallel with an integrated workflow following DNA shearing/CDNA synthesis.

Specifications:

Assay Time ~2 days (Library Prep)

Hands-On Time ~10.5 hours

Input Quantity 40 ng DNA and/or RNA

Method Targeted RNA Sequencing,Targeted DNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Somatic Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE,Low Input

System Compatibility NextSeq 550,NextSeq 500,HiSeq 2500

Technology Sequencing

Species Category Human

Cancer Type Solid Tumor

Scientific Posters:

AACR 2017: TruSight Tumor 170 and Tumor Mutational Burden

Results of analysis indicate that TruSight Tumor 170, with comprehensive coverage of cancer-related genes, shows high concordance with whole exome sequencing for accurate assessment of tumor mutational burden.

Learn More

AACR 2017: TruSight Tumor 170 and Solid Tumor Profiling Analytical Performance with FFPE Samples

Standard extractions from FFPE embedded samples provide sufficient material (40ng) in >95% of samples that were extracted by Illumina. This data shows that the TruSight Tumor 170 panel is a robust assay that generates passing sample QC data in >85% of samples with varying quality, and in >95% of samples that have quality metrics that fall within the recommendations for the kit.

Learn More

AACR 2017: TruSight Tumor 170 for Small Nucleotide Variations and Gene Amplifications in FFPE DNA Samples

TruSight Tumor 170 can achieve high sensitivity and specificity for the detection of somatic variants (small variants and CNVs) from DNA extracted from FFPE tissues.

Learn More

AACR 2017: TruSight Tumor 170 for Fusions and Splice Variants in FFPE RNA Tumor Samples

Through examining limit of detection in the context of RNA expression, this study shows that TruSight Tumor 170 provides high sensitivity and specificity in RNA variant calling down to 5 copies of transcript per ng of input.

Learn More"

"Illumina/TruSight Myeloid Sequencing Panel (96 samples)/FC-130-1010/1 Ea

" FC-130-1010 Illumina 产品编号： FC-130-1010美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The TruSight Myeloid Sequencing Panel uses expert-defined content and proven next-generation sequencing (NGS) technology to identify somatic variants in myeloid malignancies.

The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in:

Acute myeloid leukemia (AML)

Myelodysplastic syndrome (MDS)

Myeloproliferative neoplasms (MPN)

Chronic myelogenous leukemia (CML)

Chronic myelomonocytic leukemia (CMML)

Juvenile myelomonocytic leukemia (JMML)

The TruSight Myeloid Sequencing Panel covers 15 full genes (exons only) and 39 additional genes where oncogenic hotspots are covered, providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test. The result is an accurate, cost-effective solution that enables researchers to profile liquid tumors.

View the gene list

TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller. Filtering and annotation can then be performed using the following Illumina annotation and filtering tools: BaseSpace Variant Interpreter or VariantStudio.

Specifications:

Assay Time 8 hours

Hands-On Time 3 hours

Input Quantity 50 ng

System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Technology Sequencing

Variant Class Somatic Variants,Insertions-Deletions (indels)

Species Category Human

Cancer Type Hematological

Method Amplicon Sequencing,Targeted DNA Sequencing"

"Illumina/TruSeq Amplicon - Cancer Panel (96 samples)/FC-130-1008/1 Ea

" FC-130-1008 Illumina 产品编号： FC-130-1008美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The TruSeq Amplicon - Cancer Panel enables highly sensitive mutation detection studies of important cancer-related genes, including BRAF, KRAS, and EGFR. Mutations in these genes are linked to many cancers, including melanoma, colorectal, ovarian, and lung cancer.

This cancer sequencing panel provides:

A streamlined workflow including an integrated quality control assay for formalin-fixed, paraffin-embedded (FFPE) DNA

Simple sample normalization

Automated cluster generation and paired-end sequencing

On-instrument data analysis

The unique ABIlity of this assay to screen precious FFPE samples for these important variants will unlock a wealth of genomic information for many tumor types.

Genes in the TruSeq Amplicon - Cancer Panel

See which cancer-related genes are represented in this panel.

View Gene List

Specifications:

Assay Time 8 hours

Hands-On Time 3.5 hours

Input Quantity 150 ng high-quality genomic DNA,250 ng FFPE genomic DNA

System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq

Specialized Sample Types FFPE

Technology Sequencing

Variant Class Somatic Variants,Insertions-Deletions (indels)

Species Category Human

Cancer Type Pan-Cancer

Method Amplicon Sequencing,Targeted DNA Sequencing"

"Illumina/TruSight Rapid Capture (2 indexes, 8 samples, 4 enrichments)/FC-140-1102/1 Ea

" FC-140-1102 Illumina 产品编号： FC-140-1102美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London

Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets

6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

* Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 1.5 days

Input Quantity 50 ng

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Variant Class Germline Variants

Cancer Type Pan-Cancer

Method Targeted DNA Sequencing

"

"Illumina/TruSight Tumor 170 Kit, For Use with NextSeq plus Watson for Genomics/20018621/1 Ea

" 20018621 Illumina 产品编号： 20018621美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight Tumor 170, a next-generation sequencing assay designed to cover 170 genes associated with common solid tumors, is an enrichment-based targeted panel that simultaneously analyzes DNA and RNA, covering a wide range of genes and variant types. The comprehensive nature provides laboratories with a deep view into the genetics of cancer.

Comprehensive Coverage of Cancer-Related Variants

Assessment of fusions, splice variants, insertions/deletions and single-nucleotide variants (SNVs), and amplifications in one assay using DNA and RNA creates efficiencies in sample usage, time, and cost.

Accurate Results from Low-Quality Samples1

Variant detection with as little as 40 ng DNA and RNA input, and as low as 5% mutant allele frequency, maximizes the results from precious formalin-fixed paraffin-embedded (FFPE) samples.

Integrated, Streamlined Workflow

DNA and RNA are prepared in parallel with an integrated workflow following DNA shearing/CDNA synthesis.

Specifications:

Assay Time ~2 days (Library Prep)

Hands-On Time ~10.5 hours

Input Quantity 40 ng DNA and/or RNA

Method Targeted RNA Sequencing,Targeted DNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Somatic Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Specialized Sample Types FFPE,Low Input

System Compatibility NextSeq 550,NextSeq 500,HiSeq 2500

Technology Sequencing

Species Category Human

Cancer Type Solid Tumor

Scientific Posters:

AACR 2017: TruSight Tumor 170 and Tumor Mutational Burden

Results of analysis indicate that TruSight Tumor 170, with comprehensive coverage of cancer-related genes, shows high concordance with whole exome sequencing for accurate assessment of tumor mutational burden.

Learn More

AACR 2017: TruSight Tumor 170 and Solid Tumor Profiling Analytical Performance with FFPE Samples

Standard extractions from FFPE embedded samples provide sufficient material (40ng) in >95% of samples that were extracted by Illumina. This data shows that the TruSight Tumor 170 panel is a robust assay that generates passing sample QC data in >85% of samples with varying quality, and in >95% of samples that have quality metrics that fall within the recommendations for the kit.

Learn More

AACR 2017: TruSight Tumor 170 for Small Nucleotide Variations and Gene Amplifications in FFPE DNA Samples

TruSight Tumor 170 can achieve high sensitivity and specificity for the detection of somatic variants (small variants and CNVs) from DNA extracted from FFPE tissues.

Learn More

AACR 2017: TruSight Tumor 170 for Fusions and Splice Variants in FFPE RNA Tumor Samples

Through examining limit of detection in the context of RNA expression, this study shows that TruSight Tumor 170 provides high sensitivity and specificity in RNA variant calling down to 5 copies of transcript per ng of input.

Learn More"

"Illumina/TruSight RNA Pan-Cancer Panel Set A/RS-303-1002/1 Ea

" RS-303-1002 Illumina 产品编号： RS-303-1002美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

The TruSight RNA Pan-Cancer panel provides a comprehensive analysis of the cancer transcriptome. It offers:

Gene expression information, variant calling, and fusion detection with known and novel gene fusion partners

Optimized, low-input protocol for a wide range of sample types including FFPE

A comprehensive view of cancer pathways

Economical RNA sequencing (RNA-Seq) on a desktop sequencer

Highly Sensitive and Economical Targeted Sequencing

The TruSight RNA Pan-Cancer panel enables the quantitative measurement of gene expression as well as the detection of gene fusions with both known and novel gene fusion partners. The panel accommodates as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity at 8 samples per run on a desktop sequencer, allowing cost-effective access to NGS for any lab.

Intuitive Cloud-based Data Analysis

Analysis can be performed using the RNA-Seq Alignment BaseSpace App. This intuitive tool performs fusion calling andvariant detection, and provides gene expression profiles, offering a comprehensive solution to all cancer researchers.

TruSight RNA Pan-Cancer Sample Datasets

MiSeq Data

10 ng of human reference RNA (UHR), human reference brain RNA (Brain), cell line RNA (MCF7), and 20 ng of breast tumor FFPE RNA (BT) were prepared using the TruSight RNA Pan-Cancer Panel and sequenced on the MiSeq System. Read mapping and fusion calling were performed using the RNA-Seq Alignment App with STAR aligner on BaseSpace Sequence Hub.

View Project

MiniSeq Data

Human reference RNA (HBRR and UHRR), cell line RNA (MCF7), and breast tumor RNA samples were prepared using the TruSight RNA Pan-Cancer panel, and 8 samples were sequenced on the MiniSeq System at a 2x76bp read length with single indexing. The total yield was 4.2 Gb with 96.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

Access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 2.5 days

Hands-On Time 11 hours

Input Quantity 10 ng total RNA,20 - 100 ng FFPE RNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Method Targeted RNA Sequencing,Sequencing for Cytogenomics

Variant Class Gene Fusions,Somatic Variants,Novel Transcripts,Transcript Variants

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Species Category Human

Cancer Type Pan-Cancer

"

"Illumina/TruSight Inherited Disease Sequencing Panel (48 samples)/FC-121-0205/1 Ea

" FC-121-0205 Illumina 产品编号： FC-121-0205美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Developed in collaboration with Dr. Stephen Kingsmore and team during his tenure at Children's Mercy Hospital (CMH) for Pediatric Genomic Medicine, Dr. Carol Saunders at CMH, and Dr. Hilger Ropers at the Max Planck Institute

Targets 552 genes, including coding exons, intron-exon boundaries, and regions known to harbor pathogenic mutations

TruSight Inherited Disease was initially based on a 448 disease panel designed for preconception carrier testing for severe, recessive childhood diseases published by Dr. Kingsmore and team in Science Translational Medicine1.

The original content was revised by Dr. Saunders, FACMG, at CMH (following ACMG guidelines for testing ultra-rare genetic diseases) to reflect the needs of medical geneticists with a primary focus on severe recessive diseases with childhood onset. Intellectual disABIlity genes were added by Dr. Ropers.

The TruSight Inherited Disease sequencing panel set includes custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions.

Specifications:

Input Quantity 50 ng DNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Method Targeted DNA Sequencing

"

"Illumina/TruSight Tumor 15 (Library Prep Only)/OP-101-1002/1 Ea

" OP-101-1002 Illumina 产品编号： OP-101-1002美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight Tumor 15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly mutated in solid tumors. It accurately detects low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed, paraffin-embedded (FFPE) tumor tissue.

View the Gene List

Featuring a rapid workflow that can be easily integrated into lab procedures, this panel offers a single assay for accurate, economical, and rapid analysis of solid tumors.

This TruSight Tumor panel offers:

Comprehensive workflow: Assess 15 genes with one simple workflow instead of single, iterative gene testing with polymerase chain reaction (PCR)

Efficient: Rapid turnaround with only 3.5 hours of hands-on time, going from DNA to data in approximately 36 hours

Relevant gene content for solid tumors: Somatic variants selected from relevant industry guidelines1,2, key opinion leaders3,4, and pharmaceutical research

Sensitive variant detection from low DNA input: Accurate somatic variant detection of 5% allele frequency using 20 ng DNA from FFPE tissue samples

TruSight Tumor 15 Sample Datasets

3 human reference samples and 5 FFPE-exacted DNA samples from lung, colon, melanoma, and breast tumors were prepared using TruSight Tumor 15. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 150 bp read length configuration with dual indexing. The total yield was 7.6 Gb with 94.9% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

Access to this data requires a BaseSpace Sequence Hub login. 

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 7 hours

Hands-On Time 3.5 hours

Input Quantity 20 ng

System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Variant Class Somatic Variants,Insertions-Deletions (indels)

Species Category Human

Cancer Type Solid Tumor

Method Amplicon Sequencing,Targeted DNA Sequencing

"

"Illumina/TruSight RNA Fusion Panel Set B (48 samples)/RS-304-1003/1 Ea

" RS-304-1003 Illumina 产品编号： RS-304-1003美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

This targeted RNA sequencing panel is a cost-effective solution to detect gene fusions in multiple cancer types, regardless of origin. Covering 507 fusion-associated genes, a single assay enables researchers to assess most known cancer-related fusions in blood, bone marrow, and FFPE samples, with the power to identify novel fusion gene partners.

Industry-validated content for a comprehensive view of cancer-related fusion genes, detecting common and novel fusions

From RNA to results in 4 days including on-instrument software that displays fusion calls

Compatibility with desktop sequencers to maximize lab budgets

Optimized RNA sequencing for all sample types, including FFPE tissues

Simple Fusion Report for Customers New to NGS

On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman database), and evidence of fusion-supporting reads.

Robust, ReproducIBLe, and Low-Input Fusion Assay

This assay accommodates as little input as 20 ng FFPE RNA or 10 ng fresh-frozen total RNA. The TruSight RNA Fusion Panel provides a sensitive, reproducIBLe, and economical solution for studies of gene fusions in cancer research.

Specifications:

Assay Time 2.5 days

Hands-On Time 11 hours

Input Quantity 10 ng total RNA, 20–100 ng FFPE RNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Method Targeted RNA Sequencing,Sequencing for Cytogenomics

Variant Class Gene Fusions

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Species Category Human

Cancer Type Pan-Cancer"

"Illumina/TruSight Tumor 15 MiSeq Kit/OP-101-1001/1 Ea

" OP-101-1001 Illumina 产品编号： OP-101-1001美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight Tumor 15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly mutated in solid tumors. It accurately detects low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed, paraffin-embedded (FFPE) tumor tissue.

View the Gene List

Featuring a rapid workflow that can be easily integrated into lab procedures, this panel offers a single assay for accurate, economical, and rapid analysis of solid tumors.

This TruSight Tumor panel offers:

Comprehensive workflow: Assess 15 genes with one simple workflow instead of single, iterative gene testing with polymerase chain reaction (PCR)

Efficient: Rapid turnaround with only 3.5 hours of hands-on time, going from DNA to data in approximately 36 hours

Relevant gene content for solid tumors: Somatic variants selected from relevant industry guidelines1,2, key opinion leaders3,4, and pharmaceutical research

Sensitive variant detection from low DNA input: Accurate somatic variant detection of 5% allele frequency using 20 ng DNA from FFPE tissue samples

TruSight Tumor 15 Sample Datasets

3 human reference samples and 5 FFPE-exacted DNA samples from lung, colon, melanoma, and breast tumors were prepared using TruSight Tumor 15. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 150 bp read length configuration with dual indexing. The total yield was 7.6 Gb with 94.9% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

Access to this data requires a BaseSpace Sequence Hub login. 

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 7 hours

Hands-On Time 3.5 hours

Input Quantity 20 ng

System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Variant Class Somatic Variants,Insertions-Deletions (indels)

Species Category Human

Cancer Type Solid Tumor

Method Amplicon Sequencing,Targeted DNA Sequencing"

"Illumina/TruSight Cancer MiniSeq Kit/20005612/1 Ea

" 20005612 Illumina 产品编号： 20005612美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London

Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets

6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

* Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 1.5 days

Input Quantity 50 ng

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Variant Class Germline Variants

Cancer Type Pan-Cancer

Method Targeted DNA Sequencing

"

"Illumina/TruSight Rapid Capture (96 indexes, 288 samples, 24 enrichments)/FC-140-1106/1 Ea

" FC-140-1106 Illumina 产品编号： FC-140-1106美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London

Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets

6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

View Run

View Project

* Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

Specifications:

Assay Time 1.5 days

Input Quantity 50 ng

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Variant Class Germline Variants

Cancer Type Pan-Cancer

Method Targeted DNA Sequencing"

"Illumina/TruSight One Sequencing Kit (9 samples)/FC-141-1006/1 Ea

" FC-141-1006 Illumina 产品编号： FC-141-1006美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight One Sequencing Panels enable labs to expand and streamline their sequencing portfolio, while managing costs.

Two panels offer options to target up to 6700 genes associated with human disease, with high coverage

Single assay includes multiple genes of interest, replacing iterative testing

Easy, flexIBLe gene filtering and annotation options simplify BIOLOGical interpretation and reporting

These panels provide clinical research labs with an affordable solution for managing a diverse assay portfolio. The TruSight One Sequencing Panel provides comprehensive coverage of > 4800 disease-associated genes, while the TruSight One Expanded Sequencing Panel targets ~1900 additional genes with recent disease associations in the scientific literature. Investigators can choose to analyze all of the genes on the panel or focus on a specific subset. With a single assay, labs can expand existing menus, streamline workflows, or create an entire portfolio of sequencing options.

Specifications:

Input Quantity 50 ng

Content Specifications ~12 Mb genomic content for TruSight One; ~16.5 Mb genomic content for TruSight One Expanded

Multiplexing Up to 96-plex

System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Method Targeted DNA Sequencing

"

"Illumina/TruSight One Sequencing Panel (36 samples)/FC-141-1007/1 Ea

" FC-141-1007 Illumina 产品编号： FC-141-1007美  元  价： $0.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 其它>其它试剂>芯片公司分类： Clinical Research Products "Product Highlights:

TruSight One Sequencing Panels enable labs to expand and streamline their sequencing portfolio, while managing costs.

Two panels offer options to target up to 6700 genes associated with human disease, with high coverage

Single assay includes multiple genes of interest, replacing iterative testing

Easy, flexIBLe gene filtering and annotation options simplify BIOLOGical interpretation and reporting

These panels provide clinical research labs with an affordable solution for managing a diverse assay portfolio. The TruSight One Sequencing Panel provides comprehensive coverage of > 4800 disease-associated genes, while the TruSight One Expanded Sequencing Panel targets ~1900 additional genes with recent disease associations in the scientific literature. Investigators can choose to analyze all of the genes on the panel or focus on a specific subset. With a single assay, labs can expand existing menus, streamline workflows, or create an entire portfolio of sequencing options.

Specifications:

Input Quantity 50 ng

Content Specifications ~12 Mb genomic content for TruSight One; ~16.5 Mb genomic content for TruSight One Expanded

Multiplexing Up to 96-plex

System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Method Targeted DNA Sequencing

"

Illumina/TruSeq Stranded mRNA Library Prep Kit High Throughput (96 samples, 96 indexes)/RS-122-2103/1 Ea RS-122-2103 Illumina 产品编号： RS-122-2103美  元  价： $4700.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Stranded mRNA Library Prep Kit offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It is compatIBLe with a wide range of samples.

Precise and Accurate

Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Cost-Efficient

Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.

Scalable

The kit enables robust interrogation of both standard and low-quality samples, and workflows compatIBLe with a wide range of study designs.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~4.5 hours

Input Quantity 0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails)

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Species Category Other,Mammalian,Bovine,Mouse,Human,Rat

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants

Technology Sequencing

Method mRNA Sequencing

Automation CapABIlity Liquid Handling Robots"

Illumina/Nextera Rapid Capture Exome (8 rxn x 12 plex)/FC-140-1003/1 Ea FC-140-1003 Illumina 产品编号： FC-140-1003美  元  价： $10290.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome

Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful

Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 5 hours total assay time

Hands-On Time 4 hours

Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)

Mechanism of Action Mechanical fragmentation

Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit

Variant Class Small Insertions-Deletions (indels)

System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Automation CapABIlity Liquid Handling Robots

Method Exome Sequencing

"

Illumina/20-pack MiSeq Reagent Kit v2 (300-cycles)/MS-102-2022/1 Ea MS-102-2022 Illumina 产品编号： MS-102-2022美  元  价： $19900.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry

Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit

Extend your read lengths with the 500-cycle kit

Choose the perfect number of cycles for your application (50, 300, or 500)

When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:

Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)

Maximum Reads per Run Up to 15 million

Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis

System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode

Technology Sequencing"

Illumina/Nextera DNA Library Prep Kit (96 samples)/FC-121-1031/1 Ea FC-121-1031 Illumina 产品编号： FC-121-1031美  元  价： $7283.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Nextera DNA Library Preparation Kits provide a fast and easy workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. Samples prepared with Nextera kits are compatIBLe with all Illumina sequencers.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input.

See a list of automation vendors with robotic systems compatIBLe with this kit

Specifications:

Assay Time 90 minutes

Hands-On Time 15 minutes

Input Quantity 50 ng genomic DNA (compatIBLe with most large DNA genomes)

Mechanism of Action Enzymatic Fragmentation

Multiplexing Up to 96 available indexes

Species Category Human,Other,Mammalian,Mouse,Rat,Plant

Species Details CompatIBLe with most large DNA genomes.

System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiniSeq,NextSeq 500,HiSeq 2500,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants,Insertions-Deletions (indels)

Method Whole-Genome Sequencing

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)/RS-122-2001/1 Ea

" Illumina 产品编号： RS-122-2001美  元  价： $3591.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capABIlity and a simplified workflow with master-mixed reagents.

Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples (including up to 384 RNA-Seq samples on a single HiSeq 2500 System run).

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any NGS platform.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~4.5 hours

Input Quantity 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Content Specifications Captures the coding transcriptome (without strand information)

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex per lane

System Compatibility Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000

Species Category Other,Mammalian,Bovine,Mouse,Human,Rat

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants

Technology Sequencing

Method mRNA Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/TruSeq FFPE DNA Library Prep QC Kit (24 samples)/FC-121-9999/1 Ea

" Illumina 产品编号： FC-121-9999美  元  价： $155.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Custom Amplicon Low Input Library Prep Kit is a scalable amplicon sequencing solution that delivers sensitive and specific results from both low-input and formalin-fixed, paraffin-embedded (FFPE) DNA samples. It offers:

Accurate variant detection from as low as 10 ng of input genomic DNA (gDNA) and challenging FFPE samples

Fully supported, optimized workflow solution including simple data analysis

Automation-friendly workflow that can be completed in 6 hours, with only 3 hours of hands-on time

Low DNA Input and FFPE Compatibility

The TruSeq Custom Amplicon Low Input Library Prep Kit is a fully customizable, amplicon-based assay for targeted resequencing starting from as low as 10 ng of genomic DNA (gDNA). This scalable assay allows researchers to capture multiple targets of interest simultaneously and sequence up to 1536 amplicons in a single pool, using a single reaction. This library prep kit offers the flexibility to accommodate FFPE samples, such as preserved tumor tissue.

Confident Assay Design

Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio

Start a project in DesignStudio now (login required)

Assess FFPE Sample Quality before Sequencing

The TruSeq FFPE DNA Library Prep QC Kit uses a simple qPCR reaction to determine DNA quality and provide guidance on sequencing parameters. This step ensures that only samples that will achieve the necessary sequencing metrics are prepared, conserving resources that might be used on potentially low-quality, unrecoverable samples. The results of the QC step determine the recommended amount of input DNA. The TruSeq FFPE DNA Library Prep QC Kit can be bundled with the TruSeq Custom Amplicon Low Input Library Prep Kit to maximize lab budgets.

For FFPE applications, we suggest designing your project with 150 bp or 175 bp amplicons since FFPE DNA is highly degraded. TruSeq Custom Amplicon Low Input cannot be used with applications requiring 425 bp amplicons.

Species Compatibility

The TruSeq Custom Amplicon Low Input Library Prep Kit supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.

Inquire about Illumina Concierge services

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

TruSeq Custom Amplicon Library Prep Protocol:

The TruSeq Custom Amplicon assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.

Seamless Amplicon Sequencing Workflow:

The TruSeq Custom Amplicon Low Input Library Prep Kit is part of an integrated, fully supported workflow for amplicon sequencing that guides researchers from design through data analysis. Illumina technical and field specialists help ensure rapid resolution and minimize potential laboratory downtime.

Robust Variant Detection at Low DNA Input:

The TruSeq Custom Amplicon Low Input Library Prep Kit demonstrates high concordance between expected and observed variant frequencies for 10 ng high-quality FFPE DNA. Reference DNA samples were prepared following the TruSeq Custom Amplicon Low Input workflow with the TruSeq Amplicon - Cancer Panel primer pool, and sequenced on the MiSeq System in replicates of 4. Variants were called using MiSeq Reporter Software. R2 values are shown."

"Illumina/TruSeq ChIP Library Preparation Kit - Set A (12 indexes, 48 rxns)/IP-202-1012/1 Ea

" Illumina 产品编号： IP-202-1012美  元  价： $2783.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq ChIP Libary Preparation Kits provide a simple, cost-effective solution for generating chromatin immunoprecipitation sequencing (ChIP-Seq) libraries from ChIP-derived DNA. ChIP-Seq leverages next-generation sequencing (NGS) to quickly and efficiently determine the distribution and abundance of DNA-bound protein targets of interest across the genome.

This method has become one of the most widely used NGS applications, enabling researchers to reliably and simultaneously identify binding sites of a broad range of targets across the entire genome, with high resolution and without constraints.

TruSeq ChIP Library Preparation Kits are compatIBLe with all Illumina sequencers. Offering the proven data quality and ease of use of TruSeq DNA sequencing, they provide a streamlined ChIP-Seq library preparation workflow that leverages reagent master mixes to minimize pipetting and reduce total assay time.

The kits have a low DNA input requirement and are compatIBLe with a range of sample sources. In addition, they offer more robust multiplex sequencing with 24 unique indexes, allowing researchers to optimize the distribution of sequencing output across samples based on read depth requirements.

Specifications:

Assay Time ~6.5 hours

Hands-On Time ~3.5 hours

Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)

Mechanism of Action Probe hybridization, extension-ligation, and PCR

Multiplexing 1–96

Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb"

"Illumina/20-pack MiSeq Reagent Kit v2 (500-cycles)/MS-102-2023/1 Ea

" Illumina 产品编号： MS-102-2023美  元  价： $22000.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry

Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit

Extend your read lengths with the 500-cycle kit

Choose the perfect number of cycles for your application (50, 300, or 500)

When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:

Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)

Maximum Reads per Run Up to 15 million

Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis

System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode

Technology Sequencing"

"Illumina/TruSeq® Exome Kit (8 rxn × 12plex)/FC-150-1004/1 Ea

" Illumina 产品编号： FC-150-1004美  元  价： $6720.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Exome Library Prep Kit is a cost-effective library preparation and exome enrichment solution with industry-leADIng accuracy. It offers:

Pre-enrichment library pooling and optimal coverage for low-cost exome sequencing

Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and greater than or equal to 80% on-target sequencing reads

Fully supported workflow solution to simplify exome sequencing

Cost-Effective Exome Sequencing

The TruSeq Exome Library Prep Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and identify variants in less time by sequencing up to 12 libraries per flow cell lane. The high on-target percentage requires fewer sequencing cycles to reach the optimal level of coverage, while still achieving high coverage uniformity for confident results. It also enables sequencing of more exomes per run, so researchers can maximize their budgets.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Proven TruSeq Data Quality

The TruSeq Exome Library Prep Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Library Prep Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.

Convenient, Integrated Workflow Solution

Illumina provides an integrated, fully supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 2.5 days

Hands-On Time 6 hours

Input Quantity 100 ng genomic DNA

Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).

Mechanism of Action Mechanical fragmentation (COVARIS) and exome enrichment with biotinylated capture probes

Multiplexing Up to 12-plex enrichment

System Compatibility NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,NovaSeq 6000,HiSeq 4000

Specialized Sample Types FFPE

Technology Sequencing

Species Category Human

Automation CapABIlity Liquid Handling Robots

Method Exome Sequencing"

"Illumina/Nextera® XT Index Kit (96 indexes, 384 samples)/FC-131-1002/1 Ea

" Illumina 产品编号： FC-131-1002美  元  价： $999.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids

Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time

Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.

Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications

Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 90 minutes

Hands-On Time 15 minutes

Mechanism of Action Enzymatic Fragmentation

Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.

Input Quantity 1 ng DNA

Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria

System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode

Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants

Specialized Sample Types Low Input,Single Cells

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/Nextera DNA CD Indexes (24 Indexes, 24 Samples)/20018707/1 Ea

" Illumina 产品编号： 20018707美  元  价： $120.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time

Flexibility to accommodate variations in sample type, DNA input amount, and application

Optimized library prep performance, generating reliable results

Save Time and Resources

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:

Assay Time ~3.5 hours total assay time

Hands-On Time 1-1.5 hours

Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng

Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome

Mechanism of Action Bead-linked transposome

Multiplexing Up to 96 available indexes

Species Category Any Species

System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Method Whole-Genome Sequencing

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/TruSeq Dual Index Sequencing Primer Box, Single-Read/FC-121-1003/1 Ea

" Illumina 产品编号： FC-121-1003美  元  价： $95.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

The primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 2500, HiSeq 2000, HiSeq 1500, HiSeq 1000 Systems; the HiScanSQ System using TruSeq SBS Kit v3; or the Genome Analyzer System.

The single-read kit should be used with a single-read flow cell, and the paired-end kit with a paired-end flow cell.

Any Nextera library except Nextera Mate Pair

Any library constructed with a Nextera or Nextera XT Index Kit

Any TruSeq HT library when performing a dual-index run on a single-read flow cell

A TruSeq Synthetic Long-Read DNA library

Any TruSight library except TruSight RNA Pan-Cancer 

A TruSeq Rapid Exome library

A TruSeq Custom Amplicon library (v1.5 and Low Input) 

A TruSeq Amplicon Cancer Panel library

A VeriSeq PGS library"

"Illumina/MiniSeq Mid Output Kit (300-cycles)/FC-420-1004/1 Ea

" Illumina 产品编号： FC-420-1004美  元  价： $550.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

MiniSeq System sequencing reagent kits bring the power and reliABIlity of proven Illumina next-generation sequencing (NGS) to labs of all sizes. Access cost-efficient sequencing with high data quality, even for low numbers of samples. These kits offer:

Robust base calling and optimal signal-to-noise ratio

Multiple options for both sequencing output and read length

Simple workflow that reduces chance for error through the integration of clustering, sequencing and wash reagents into a single cartridge

Intuitive labeling and RFID-encoded reagents

Optimized chemistry with the latest in Illumina sequencing by synthesis (SBS) chemistry

Configurations for various applications

MiniSeq Reagent Kits offer a wide range of solutions for applications ranging from gene expression and small RNA profiling to targeted DNA amplicon sequencing and small genome sequencing. High accuracy and paired-end sequencing capABIlity are built into every reagent kit.

Ready-to-use cartridges redefine ease of use

Preconfigured reagent kits for the MiniSeq System provide all the components needed for cluster generation, sequencing and wash on the platform in 1 premixed, integrated, RFID-enabled cartridge. Simply load user-prepared library pools directly into the MiniSeq System. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Specifications:

Maximum Output 7.5 Gb (300-cycle high output kit), 3.75 Gb (150-cycle high output kit), 2.4 Gb (300-cycle mid output kit), 1.875 Gb (75-cycle high output kit)

Maximum Reads Per Run Up to 25 million (high output kits), Up to 8 million (mid output kits)

Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis

Technology Sequencing

System Compatibility MiniSeq"

"Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold High Throughput (96 samples, 96 indexes)/RS-122-

" Illumina 产品编号： RS-122-2303美  元  价： $10280.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs

The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.

TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.

The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.

Species Compatibility

The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:

Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants

Specialized Sample Types FFPE

Method Whole-Transcriptome Sequencing

Species Category Mouse,Human,Rat

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/TruSeq Nano DNA LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)/FC-121-4001/1 Ea

" Illumina 产品编号： FC-121-4001美  元  价： $735.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Nano DNA Library Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted library preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

Designed for low sample input

High coverage quality

Accelerated library preparation

Manual preparation of high-quality libraries in less than a day

The proven TruSeq DNA library preparation workflow has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples

The TruSeq Nano DNA protocol offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps

TruSeq Nano DNA kits reduce the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of trADItionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. TruSeq Nano DNA kits are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexIBLe throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq Nano DNA LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.

TruSeq Nano DNA HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).

TruSeq Nano DNA Library Preparation Kits are also available for use with the automated NeoPrep Library Prep System.

Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Shotgun Sequencing,Whole-Genome Sequencing,Genotyping by Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Species Category Mammalian,Mouse,Human,Other,Rat,Plant

System Compatibility NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 2000,MiSeq,HiSeq X Ten,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000

Specialized Sample Types Low Input

Technology Sequencing

Automation CapABIlity NeoPrep Digital Microfluidics,Liquid Handling Robots"

"Illumina/ForenSeq DNA Signature Prep Kit/TG-450-1001/1 Ea

" Illumina 产品编号： TG-450-1001美  元  价： $15500.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The ForenSeq DNA Signature Prep Kit is part of a complete, fully validated DNA-to-data solution designed for forensic genomics applications. It includes all reagents to prepare next-generation sequencing (NGS) libraries from forensic DNA samples.

Achieve high resolution and exceptional accuracy from as little as 1 ng of DNA—Even with complex mixtures or degraded DNA. This kit allows labs to:

Perform multiplexing and rapid sample processing

Access a wider range of informative single-nucleotide polymorphisms (SNPs) with a single kit

Analyze challenging samples

Multiplexing and rapid sample processing

The ForenSeq DNA Signature Prep Kit supports preparation of up to 96 libraries simultaneously using a simple plate-based format and standard lab equipment. Targeted primer mixes enable analysis of autosomal, Y- and X-chromosome short tandem repeat (STR) targets, and identity-informative SNPs, all in a single reaction.

Optionally, you can include biogeographical ancestry-informative SNPs (aiSNPs) and phenotypic-informative SNPs (piSNPs). This data can be critical in generating investigative leads from “no sUSPect” cases that have otherwise gone cold.

A wider range of informative SNPs with a single kit

The ForenSeq DNA Signature Prep Kit consolidates all autosomal STR Markers currently used around the world for casework and criminal DNA databases. This single, streamlined workflow eliminates the need to run multiple STR tests.

The kit delivers approximately 200 genetic Markers in a single test, removing the tradeoffs and risk imposed by technical limitations such as low DNA quantity. It also contains a dense set of identity informative single nucleotide polymorphism (iiSNP) Marker sets not routinely available with trADItional capillary electrophoresis (CE) methods.

Superior analysis of challenging samples

Cases are often complicated and sometimes unresolved due to the presence of highly degraded DNA, low quality DNA, or complex DNA mixtures. The ForenSeq DNA Signature Prep Kit includes a large number of Markers, many of which are highly polymorphic.

These additional Markers, coupled with the inherent sensitivity of Illumina chemistry, help detect minor components that might go undetected by conventional STR and CE analysis.

Specifications:

Assay Time ~6.5 hours

Hands-On Time ~3.5 hours

Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)

Mechanism of Action Probe hybridization, extension-ligation, and PCR

Multiplexing 1–96

Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb

System Compatibility NovaSeq 5000,HiSeq 2000,HiScanSQ,MiSeq FGx

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Short Tandem Repeats (STRs)

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Method Amplicon Sequencing,Targeted DNA Sequencing

ForenSeq DNA Signature Prep Kit Workflow:

The ForenSeq DNA Signature Prep Kit is part of a fully integrated, sample-to-answer solution, including library preparation, DNA sequencing platform, and data analysis software specifically designed for forensic genomics.

ForenSeq DNA Signature Prep Kit Forensic Loci and Investigative Workflow:

With ~200 genetic Markers in a single workflow, the MiSeq FGx System offers the most comprehensive multiplex of STRs and SNPs and the most straightforward path to human identification."

"Illumina/TruSeq Synthetic Long-Read DNA Barcode Kit (4 barcode plates)/FC-126-1003/1 Ea

" Illumina 产品编号： FC-126-1003美  元  价： $3045.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end sequencing solution that can be used for genome assembly or genome phasing. Depending on the analysis option you select, you can use it to:

Assemble synthetically long reads for de novo assembly and genome finishing applications

Perform genome phasing to identify co-inherited alleles and haplotype information, as well as phase de novo mutations

Accurately Construct Synthetic Long Reads

The TruSeq Synthetic Long-Read DNA Library Prep and TruSeq Synthetic Long-Read DNA Barcode Kits are designed for preparing DNA libraries to generate synthetically long reads. The library prep kit combines TruSeq and Nextera chemistries with synthetic long-read technology to prepare DNA libraries. The accompanying barcode kit includes 384 indexes for labeling the samples in each well. These indexes are then used after sequencing to construct synthetically long fragments for long-read assembly and phasing analysis.

Simplified Informatics for Genome Assembly or Human Whole-Genome Phasing

After sequencing, push-button analysis in BaseSpace Sequence Hub simplifies assembly of long reads. Data can be transferred from an Illumina sequencing instrument to the BaseSpace Sequence Hub cloud instantly.

BaseSpace App for Genome Assembly:

The TruSeq Long-Read Assembly App constructs long, synthetic reads from shorter sequencing reads for accurate genome assembly and genome finishing. TruSeq synthetic long-read technology allows you to use the same familiar platform with a new application.

BaseSpace App for Phasing Analysis:

The TruSeq Phasing Analysis App can be used to perform whole human genome phasing, identifying haplotype information and co-inherited alleles, and phasing de novo mutations. By constructing synthetically long fragments from shorter sequencing reads, this method provides more comprehensive and accurate phasing compared to conventional trio studies or statistical inference.

Specifications:

Assay Time 3 days

Hands-On Time 6 hours

Species Category Any Species

System Compatibility HiSeq 2000,HiSeq 3000,HiSeq 2500,HiSeq 4000

Method Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing

Technology Sequencing

品牌介绍

"

"Illumina/TruSeq® Rapid Exome Kit (8 rxn × 12 plex)/FC-144-1004/1 Ea

" Illumina 产品编号： FC-144-1004美  元  价： $9600.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Rapid Exome Library Prep Kit delivers a simple, efficient method for high-confidence calling of exonic variants. The fast library preparation and exome enrichment workflow deliver libraries in 1 day with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. This kit offers:

Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment.

Rapid, automation-friendly workflow completes in 1 day with only 3 hours of hands-on time.

High coverage uniformity across exonic regions and on-target %

Proven TruSeq Data Quality

The TruSeq Rapid Exome Library Prep Kit delivers an average of 75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels, but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 1 day

Hands-On Time 3 hours

Input Quantity 50 ng genomic DNA

Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).

Mechanism of Action Transposase-based fragmentation and exome enrichment with biotinylated capture probes

Multiplexing Up to 12-plex enrichment

Technology Sequencing

Species Category Human

Automation CapABIlity Liquid Handling Robots

System Compatibility NextSeq 500,NovaSeq 6000,HiSeq 4000

Method Exome Sequencing

"

"Illumina/Flex Lysis Reagent Kit/20018706/1 Ea

" Illumina 产品编号： 20018706美  元  价： $288.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time

Flexibility to accommodate variations in sample type, DNA input amount, and application

Optimized library prep performance, generating reliable results

Save Time and Resources

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:

Assay Time ~3.5 hours total assay time

Hands-On Time 1-1.5 hours

Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng

Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome

Mechanism of Action Bead-linked transposome

Multiplexing Up to 96 available indexes

Species Category Any Species

System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Method Whole-Genome Sequencing

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/TruSeq Nano DNA HT Library Preparation Kit (96 indexes in plate format, 96 samples)/FC-121-4003/1 Ea

" Illumina 产品编号： FC-121-4003美  元  价： $3000.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Nano DNA Library Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted library preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

Designed for low sample input

High coverage quality

Accelerated library preparation

Manual preparation of high-quality libraries in less than a day

The proven TruSeq DNA library preparation workflow has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples

The TruSeq Nano DNA protocol offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps

TruSeq Nano DNA kits reduce the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of trADItionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. TruSeq Nano DNA kits are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexIBLe throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq Nano DNA LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.

TruSeq Nano DNA HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).

TruSeq Nano DNA Library Preparation Kits are also available for use with the automated NeoPrep Library Prep System.

Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Shotgun Sequencing,Whole-Genome Sequencing,Genotyping by Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Species Category Mammalian,Mouse,Human,Other,Rat,Plant

System Compatibility NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 2000,MiSeq,HiSeq X Ten,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000

Specialized Sample Types Low Input

Technology Sequencing

Automation CapABIlity NeoPrep Digital Microfluidics,Liquid Handling Robots"

"Illumina/TruSeq SBS Kit v3-HS (200 cycles)/FC-401-3001/1 Ea

" Illumina 产品编号： FC-401-3001美  元  价： $8600.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

TruSeq SBS v3-HS kits contain ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis (SBS) technology on HiSeq sequencing systems. These kits offer a hands-on preparation time of only two minutes.

To perform a TruSeq v3 run on an enabled HiSeq system, you need one TruSeq SBS Kit v3. Each kit includes sufficient reagents for sequencing 1 flow cell.

The TruSeq v3 reagent kits can be run using either single-read (SR) or paired-end (PE) reads.

The TruSeq PE Cluster Kit v3-cBot-HS enables up to 600 Gb of output, while the TruSeq SR Cluster Kit v3-cBot-HS enables up to 300 Gb of output.

Note: This kit is NOT compatIBLe with the Genome Analyzer.

Specifications:

Maximum Output Up to 600 Gb (with HiSeq 2500 dual flow cell)

Maximum Reads per Run Up to 6 billion paired-end reads (with HiSeq 2500 dual flow cell)

Reagent Type Paired-End Sequencing,Sequencing by Synthesis,Single-Read Sequencing

System Compatibility HiSeq 2000,HiScanSQ,HiSeq 1000,HiSeq 2500

Technology Sequencing"

"Illumina/TruSeq Synthetic Long-Read DNA Accessory Kit/FC-126-1004/1 Ea

" Illumina 产品编号： FC-126-1004美  元  价： $81.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end sequencing solution that can be used for genome assembly or genome phasing. Depending on the analysis option you select, you can use it to:

Assemble synthetically long reads for de novo assembly and genome finishing applications

Perform genome phasing to identify co-inherited alleles and haplotype information, as well as phase de novo mutations

Accurately Construct Synthetic Long Reads

The TruSeq Synthetic Long-Read DNA Library Prep and TruSeq Synthetic Long-Read DNA Barcode Kits are designed for preparing DNA libraries to generate synthetically long reads. The library prep kit combines TruSeq and Nextera chemistries with synthetic long-read technology to prepare DNA libraries. The accompanying barcode kit includes 384 indexes for labeling the samples in each well. These indexes are then used after sequencing to construct synthetically long fragments for long-read assembly and phasing analysis.

Simplified Informatics for Genome Assembly or Human Whole-Genome Phasing

After sequencing, push-button analysis in BaseSpace Sequence Hub simplifies assembly of long reads. Data can be transferred from an Illumina sequencing instrument to the BaseSpace Sequence Hub cloud instantly.

BaseSpace App for Genome Assembly:

The TruSeq Long-Read Assembly App constructs long, synthetic reads from shorter sequencing reads for accurate genome assembly and genome finishing. TruSeq synthetic long-read technology allows you to use the same familiar platform with a new application.

BaseSpace App for Phasing Analysis:

The TruSeq Phasing Analysis App can be used to perform whole human genome phasing, identifying haplotype information and co-inherited alleles, and phasing de novo mutations. By constructing synthetically long fragments from shorter sequencing reads, this method provides more comprehensive and accurate phasing compared to conventional trio studies or statistical inference.

Specifications:

Assay Time 3 days

Hands-On Time 6 hours

Species Category Any Species

System Compatibility HiSeq 2000,HiSeq 3000,HiSeq 2500,HiSeq 4000

Method Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing

Technology Sequencing

品牌介绍

"

"Illumina/TruSeq DNA PCR-Free LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)/FC-121-3001/1 Ea

" Illumina 产品编号： FC-121-3001美  元  价： $735.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole-genomes. The kits offer:

Shortened gel-free workflows that remove the need for PCR

ABIlity to sequence the most challenging regions

Improved genome coverage to identify the greatest number of variants

Sequence the most challenging regions

TruSeq DNA PCR-free kits offer superior coverage of areas which are trADItionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content.

The kits are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Detect the greatest number of variants

PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high G/C rich regions.

Use PCR-free for faster protocols

Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, the TruSeq DNA PCR-Free Library Preparation Kit provides a range of enhancements to the industry's most widely adopted library preparation workflow.

Access flexIBLe throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq DNA PCR-Free LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.

TruSeq DNA PCR-Free HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).

Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:

Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000

Automation CapABIlity Liquid Handling Robots

Variant Class Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions

Species Category Rat,Mouse

Method Whole-Transcriptome Sequencing

Technology Sequencing"

"Illumina/TruSeq Stranded Total RNA Kit with Ribo-Zero Plant, Set B (48 samples, 12 indexes)/RS-122-2402/1 Ea

" Illumina 产品编号： RS-122-2402美  元  价： $5630.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

High-Quality Whole-Transcriptome Analysis with Precise Strand Information

RNA-Seq technology provides uniform coverage, precise measurement of strand orientation and high-confidence discovery of features such as alternative transcripts, antisense expression and allele-specific expression across both coding and multiple forms of noncoding RNA. The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Efficient Ribosomal RNA Reduction Across a Range of Plant Species, Study Designs

The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. The kit has been validated for use in multiple plant species, including ArABIdopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:

Assay Time ~6.5 hours

Hands-On Time ~3.5 hours

Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)

Mechanism of Action Probe hybridization, extension-ligation, and PCR

Multiplexing 1–96

Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb

Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)

System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000

Species Category Other,Bovine,Mouse,Human,Rat

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Method Amplicon Sequencing,Custom Sequencing

"

"Illumina/Nextera Rapid Capture Exome (8 rxn x 1 plex)/FC-140-1000/1 Ea

" Illumina 产品编号： FC-140-1000美  元  价： $2573.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome

Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful

Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 5 hours total assay time

Hands-On Time 4 hours

Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)

Mechanism of Action Mechanical fragmentation

Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit

Variant Class Small Insertions-Deletions (indels)

System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Automation CapABIlity Liquid Handling Robots

Method Exome Sequencing

"

"Illumina/TruSeq ChIP Sample Preparation Kit - Set B (12 indexes, 48 rxns)/IP-202-1024/1 Ea

" Illumina 产品编号： IP-202-1024美  元  价： $2783.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq ChIP Libary Preparation Kits provide a simple, cost-effective solution for generating chromatin immunoprecipitation sequencing (ChIP-Seq) libraries from ChIP-derived DNA. ChIP-Seq leverages next-generation sequencing (NGS) to quickly and efficiently determine the distribution and abundance of DNA-bound protein targets of interest across the genome.

This method has become one of the most widely used NGS applications, enabling researchers to reliably and simultaneously identify binding sites of a broad range of targets across the entire genome, with high resolution and without constraints.

TruSeq ChIP Library Preparation Kits are compatIBLe with all Illumina sequencers. Offering the proven data quality and ease of use of TruSeq DNA sequencing, they provide a streamlined ChIP-Seq library preparation workflow that leverages reagent master mixes to minimize pipetting and reduce total assay time.

The kits have a low DNA input requirement and are compatIBLe with a range of sample sources. In addition, they offer more robust multiplex sequencing with 24 unique indexes, allowing researchers to optimize the distribution of sequencing output across samples based on read depth requirements.

Specifications:

Assay Time ~6.5 hours

Hands-On Time ~3.5 hours

Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)

Mechanism of Action Probe hybridization, extension-ligation, and PCR

Multiplexing 1–96

Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb"

"Illumina/Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)/FC-131-2003/1 Ea

" Illumina 产品编号： FC-131-2003美  元  价： $989.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids

Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time

Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.

Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications

Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 90 minutes

Hands-On Time 15 minutes

Mechanism of Action Enzymatic Fragmentation

Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.

Input Quantity 1 ng DNA

Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria

System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode

Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants

Specialized Sample Types Low Input,Single Cells

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/TruSeq Synthetic Long-Read DNA Barcode Kit (1 barcode plate)/FC-126-1002/1 Ea

" Illumina 产品编号： FC-126-1002美  元  价： $761.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end sequencing solution that can be used for genome assembly or genome phasing. Depending on the analysis option you select, you can use it to:

Assemble synthetically long reads for de novo assembly and genome finishing applications

Perform genome phasing to identify co-inherited alleles and haplotype information, as well as phase de novo mutations

Accurately Construct Synthetic Long Reads

The TruSeq Synthetic Long-Read DNA Library Prep and TruSeq Synthetic Long-Read DNA Barcode Kits are designed for preparing DNA libraries to generate synthetically long reads. The library prep kit combines TruSeq and Nextera chemistries with synthetic long-read technology to prepare DNA libraries. The accompanying barcode kit includes 384 indexes for labeling the samples in each well. These indexes are then used after sequencing to construct synthetically long fragments for long-read assembly and phasing analysis.

Simplified Informatics for Genome Assembly or Human Whole-Genome Phasing

After sequencing, push-button analysis in BaseSpace Sequence Hub simplifies assembly of long reads. Data can be transferred from an Illumina sequencing instrument to the BaseSpace Sequence Hub cloud instantly.

BaseSpace App for Genome Assembly:

The TruSeq Long-Read Assembly App constructs long, synthetic reads from shorter sequencing reads for accurate genome assembly and genome finishing. TruSeq synthetic long-read technology allows you to use the same familiar platform with a new application.

BaseSpace App for Phasing Analysis:

The TruSeq Phasing Analysis App can be used to perform whole human genome phasing, identifying haplotype information and co-inherited alleles, and phasing de novo mutations. By constructing synthetically long fragments from shorter sequencing reads, this method provides more comprehensive and accurate phasing compared to conventional trio studies or statistical inference.

Specifications:

Assay Time 3 days

Hands-On Time 6 hours

Species Category Any Species

System Compatibility HiSeq 2000,HiSeq 3000,HiSeq 2500,HiSeq 4000

Method Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing

Technology Sequencing

品牌介绍

"

"Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat High Throughput (96 samples, 96 index

" Illumina 产品编号： RS-122-2203美  元  价： $10280.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs

The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.

TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.

The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.

Species Compatibility

The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:

Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants

Specialized Sample Types FFPE

Method Whole-Transcriptome Sequencing

Species Category Mouse,Human,Rat

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/TruSeq Nano DNA Library Prep Kit for NeoPrep (16 indexes, 16 samples)/NP-101-1001/1 Ea

" Illumina 产品编号： NP-101-1001美  元  价： $480.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Nano DNA for NeoPrep offers a simple, all-inclusive prep solution, ideal for any whole-genome sequencing application. Run the same proven TruSeq Nano DNA biochemistry on the fully integrated digital microfluidics NeoPrep Library Prep System.

Simplified and seamless workflow solution – Includes onboard quantification and normalization to deliver sequencing-ready libraries

Exceptional performance and reproducibility – Generates libraries with TruSeq Nano DNA coverage and quality, with minimal hands-on time and reduced user variABIlity

Low input requirement – Requires as little as 25 ng genomic DNA to prepare high-quality, sequencing-ready libraries

Reduce Library Bias and Coverage Gaps

TruSeq Nano DNA library prep reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. It also provides excellent coverage of trADItionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables you to access more information from each sequencing run.

NeoPrep System enhances TruSeq Nano DNA performance

The NeoPrep System enhances TruSeq Nano DNA performance by providing high-quality reproducIBLe results, even with low input amounts of DNA. Digital microfluidics technology precisely manipulates droplets that perform the library prep workflow, including quantification and normalization within the tightly controlled environment of the NeoPrep library card.

A simple, intuitive workflow delivers 16 sequencing-ready libraries, eliminating almost all manual steps, and reducing hands-on time from ~4 hours to just 30 minutes. In addition, digital microfluidics requires less DNA input, enabling excellent performance from 25-75 ng of genomic DNA. Successful libraries have been demonstrated with DNA inputs ranging from 1–100 ng, up to 10-fold lower than required by manual protocols.

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Shotgun Sequencing,Whole-Genome Sequencing,Genotyping by Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Species Category Mammalian,Mouse,Human,Other,Rat,Plant

System Compatibility NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 2000,MiSeq,HiSeq X Ten,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000

Specialized Sample Types Low Input

Technology Sequencing

Automation CapABIlity NeoPrep Digital Microfluidics"

"Illumina/Nextera Rapid Capture Exome (4 rxn x 12 plex)/FC-140-1002/1 Ea

" Illumina 产品编号： FC-140-1002美  元  价： $7665.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome

Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful

Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 5 hours total assay time

Hands-On Time 4 hours

Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)

Mechanism of Action Mechanical fragmentation

Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit

Variant Class Small Insertions-Deletions (indels)

System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Automation CapABIlity Liquid Handling Robots

Method Exome Sequencing

"

"Illumina/MiSeq Reagent Nano Kit v2 (300-cycles)/MS-103-1001/1 Ea

" Illumina 产品编号： MS-103-1001美  元  价： $290.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry

Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit

Extend your read lengths with the 500-cycle kit

Choose the perfect number of cycles for your application (50, 300, or 500)

When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:

Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)

Maximum Reads per Run Up to 15 million

Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis

System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode

Technology Sequencing"

"Illumina/HiSeq PE Cluster Kit V4 - cBot/PE-401-4001/1 Ea

" Illumina 产品编号： PE-401-4001美  元  价： $6200.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

The HiSeq PE (Paired-End) Cluster Kit v4 cBot provides reagents for the cBot cluster amplification system. DNA library samples are bound to complimentary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridization primer by 3’ extension using a high fidelity DNA polymerase. Copies are isothermally amplified to create clonal clusters of ~1000 copies each, ready for sequencing on enabled HiSeq systems*.

Cluster generation reagents provided in a pre-mixed, 96 well plate format that requires minimal reagent preparation. The kit also included reagents for cluster resynthesis of the reverse strand, regenerated by bridge amplification within the paired-end flow cell. After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read."

"Illumina/Nextera DNA Flex Library Prep (96 Samples)/20018705/1 Ea

" Illumina 产品编号： 20018705美  元  价： $4032.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time

Flexibility to accommodate variations in sample type, DNA input amount, and application

Optimized library prep performance, generating reliable results

Save Time and Resources

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:

Assay Time ~3.5 hours total assay time

Hands-On Time 1-1.5 hours

Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng

Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome

Mechanism of Action Bead-linked transposome

Multiplexing Up to 96 available indexes

Species Category Any Species

System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Method Whole-Genome Sequencing

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat Set B (48 samples, 12 indexes)/RS-122

" Illumina 产品编号： RS-122-2202美  元  价： $5630.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs

The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.

TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.

The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.

Species Compatibility

The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:

Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants

Specialized Sample Types FFPE

Method Whole-Transcriptome Sequencing

Species Category Mouse,Human,Rat

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/TruSeq DNA PCR-Free HT Library Preparation Kit (96 indexes in plate format, 96 samples)/FC-121-3003/1 Ea

" Illumina 产品编号： FC-121-3003美  元  价： $3000.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole-genomes. The kits offer:

Shortened gel-free workflows that remove the need for PCR

ABIlity to sequence the most challenging regions

Improved genome coverage to identify the greatest number of variants

Sequence the most challenging regions

TruSeq DNA PCR-free kits offer superior coverage of areas which are trADItionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content.

The kits are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Detect the greatest number of variants

PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high G/C rich regions.

Use PCR-free for faster protocols

Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, the TruSeq DNA PCR-Free Library Preparation Kit provides a range of enhancements to the industry's most widely adopted library preparation workflow.

Access flexIBLe throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq DNA PCR-Free LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.

TruSeq DNA PCR-Free HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).

Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:

Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000

Automation CapABIlity Liquid Handling Robots

Variant Class Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions

Species Category Rat,Mouse

Method Whole-Transcriptome Sequencing

Technology Sequencing"

"Illumina/Nextera DNA CD Indexes (96 Indexes, 96 Samples)/20018708/1 Ea

" Illumina 产品编号： 20018708美  元  价： $480.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time

Flexibility to accommodate variations in sample type, DNA input amount, and application

Optimized library prep performance, generating reliable results

Save Time and Resources

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:

Assay Time ~3.5 hours total assay time

Hands-On Time 1-1.5 hours

Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng

Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome

Mechanism of Action Bead-linked transposome

Multiplexing Up to 96 available indexes

Species Category Any Species

System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Method Whole-Genome Sequencing

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/Nextera Rapid Capture Expanded Exome (4 rxn x 12 plex)/FC-140-1005/1 Ea

" Illumina 产品编号： FC-140-1005美  元  价： $8946.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome

Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful

Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 5 hours total assay time

Hands-On Time 4 hours

Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)

Mechanism of Action Mechanical fragmentation

Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit

Variant Class Small Insertions-Deletions (indels)

System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Automation CapABIlity Liquid Handling Robots

Method Exome Sequencing

"

"Illumina/TruSeq Custom Amplicon Filter Plate (1 plate)/FC-130-1006/1 Ea

" Illumina 产品编号： FC-130-1006美  元  价： $51.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. The assay enables you to focus interrogation on key regions of genomic interest. Up to 1536 amplicons can be sequenced in a single reaction using a simple workflow. This highly targeted approach offers unparalleled efficiency for discovering, validating, and screening genetic variants in areas of research focus.

Requiring as little as 50 ng of input genomic DNA (gDNA), this kit can be used with a wide range of sample types. The ABIlity to multiplex up to 1536 amplicons per reaction allows coverage of up to 650 kb of cumulative sequence. Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling you to fully benefit from desktop sequencer output capABIlities.

This assay provides improved performance in difficult to address GC-rich regions, as well as increased library yield, uniformity, and stABIlity. Additionally, optimized amplification steps enable sample input requirements to drop from 150 ng to 50 ng.

Simplified Probe Design

Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio

Start a project in DesignStudio now (login required)

Simple Data Analysis

Automated data analysis using the BaseSpace TruSeq Amplicon App allows researchers to easily review project data, including variants detected, across multiple runs.

Species Compatibility

TruSeq Custom Amplicon supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.

Inquire about Illumina Concierge services

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)

System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000

Species Category Other,Bovine,Mouse,Human,Rat

Specialized Sample Types FFPE

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Method Amplicon Sequencing,Custom Sequencing"

"Illumina/Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)/FC-131-2004/1 Ea

" Illumina 产品编号： FC-131-2004美  元  价： $989.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids

Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time

Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.

Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications

Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 90 minutes

Hands-On Time 15 minutes

Mechanism of Action Enzymatic Fragmentation

Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.

Input Quantity 1 ng DNA

Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria

System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode

Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants

Specialized Sample Types Low Input,Single Cells

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/NextSeq 500/550 High Output v2 kit (300 cycles)/FC-404-2004/1 Ea

" Illumina 产品编号： FC-404-2004美  元  价： $4450.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

NextSeq 500/550 v2 sequencing reagent kits bring the power of a high-throughput sequencing system to the desktop, now with improved data quality and the highest yield of error-free reads. These kits offer:

Robust base calling and improved signal-to-noise ratio

Multiple options for both sequencing output and read length

Simplified reagent cartridge configurations, with improved workflow and cartridge loADIng

Intuitive labeling and RFID-encoded reagents

Optimized chemistry with the latest in sequencing by synthesis (SBS) and bridge-amplification cluster generation

The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Ready-to-use cartridges redefine ease-of-use

Pre-configured reagent kits for the NextSeq Series Systems provide all the components needed for sequencing on the platform in 3 ready-to-use cartridges (reagent, flow cell, and buffer). The kits offer simplified cartridge loADIng with the bleach and dual-indexing primers already in the cartridge.

Simply load user-prepared library pools directly into the NextSeq reagent cartridge. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

Specifications:

Maximum Output 120 Gb (300-cycle high output kit), 60 Gb (150-cycle high output kit), 39 Gb (300-cycle mid output kit), 30 Gb (75-cycle high output kit), 19.5 Gb (150-cycle mid output kit)

Maximum Reads per Run 400 million clusters (high output kits), 130 million clusters (mid output kits)

Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis,Single-Read Sequencing

System Compatibility NextSeq 550,NextSeq 500

Technology Sequencing"

"Illumina/MiniSeq High Output Reagent Kit (150-cycles)/FC-420-1002/1 Ea

" Illumina 产品编号： FC-420-1002美  元  价： $965.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

MiniSeq System sequencing reagent kits bring the power and reliABIlity of proven Illumina next-generation sequencing (NGS) to labs of all sizes. Access cost-efficient sequencing with high data quality, even for low numbers of samples. These kits offer:

Robust base calling and optimal signal-to-noise ratio

Multiple options for both sequencing output and read length

Simple workflow that reduces chance for error through the integration of clustering, sequencing and wash reagents into a single cartridge

Intuitive labeling and RFID-encoded reagents

Optimized chemistry with the latest in Illumina sequencing by synthesis (SBS) chemistry

Configurations for various applications

MiniSeq Reagent Kits offer a wide range of solutions for applications ranging from gene expression and small RNA profiling to targeted DNA amplicon sequencing and small genome sequencing. High accuracy and paired-end sequencing capABIlity are built into every reagent kit.

Ready-to-use cartridges redefine ease of use

Preconfigured reagent kits for the MiniSeq System provide all the components needed for cluster generation, sequencing and wash on the platform in 1 premixed, integrated, RFID-enabled cartridge. Simply load user-prepared library pools directly into the MiniSeq System. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Specifications:

Maximum Output 7.5 Gb (300-cycle high output kit), 3.75 Gb (150-cycle high output kit), 2.4 Gb (300-cycle mid output kit), 1.875 Gb (75-cycle high output kit)

Maximum Reads Per Run Up to 25 million (high output kits), Up to 8 million (mid output kits)

Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis

Technology Sequencing

System Compatibility MiniSeq"

"Illumina/TruSeq® Exome Kit (8 rxn × 6 plex)/FC-150-1002/1 Ea

" Illumina 产品编号： FC-150-1002美  元  价： $3360.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Exome Library Prep Kit is a cost-effective library preparation and exome enrichment solution with industry-leADIng accuracy. It offers:

Pre-enrichment library pooling and optimal coverage for low-cost exome sequencing

Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and greater than or equal to 80% on-target sequencing reads

Fully supported workflow solution to simplify exome sequencing

Cost-Effective Exome Sequencing

The TruSeq Exome Library Prep Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and identify variants in less time by sequencing up to 12 libraries per flow cell lane. The high on-target percentage requires fewer sequencing cycles to reach the optimal level of coverage, while still achieving high coverage uniformity for confident results. It also enables sequencing of more exomes per run, so researchers can maximize their budgets.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Proven TruSeq Data Quality

The TruSeq Exome Library Prep Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Library Prep Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.

Convenient, Integrated Workflow Solution

Illumina provides an integrated, fully supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 2.5 days

Hands-On Time 6 hours

Input Quantity 100 ng genomic DNA

Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).

Mechanism of Action Mechanical fragmentation (COVARIS) and exome enrichment with biotinylated capture probes

Multiplexing Up to 12-plex enrichment

System Compatibility NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,NovaSeq 6000,HiSeq 4000

Specialized Sample Types FFPE

Technology Sequencing

Species Category Human

Automation CapABIlity Liquid Handling Robots

Method Exome Sequencing"

"Illumina/Infinium FFPE QC Kit (384 reactions)/WG-321-1001/1 Ea

" Illumina 产品编号： WG-321-1001美  元  价： $99.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. The assay enables you to focus interrogation on key regions of genomic interest. Up to 1536 amplicons can be sequenced in a single reaction using a simple workflow. This highly targeted approach offers unparalleled efficiency for discovering, validating, and screening genetic variants in areas of research focus.

Requiring as little as 50 ng of input genomic DNA (gDNA), this kit can be used with a wide range of sample types. The ABIlity to multiplex up to 1536 amplicons per reaction allows coverage of up to 650 kb of cumulative sequence. Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling you to fully benefit from desktop sequencer output capABIlities.

This assay provides improved performance in difficult to address GC-rich regions, as well as increased library yield, uniformity, and stABIlity. Additionally, optimized amplification steps enable sample input requirements to drop from 150 ng to 50 ng.

Simplified Probe Design

Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio

Start a project in DesignStudio now (login required)

Simple Data Analysis

Automated data analysis using the BaseSpace TruSeq Amplicon App allows researchers to easily review project data, including variants detected, across multiple runs.

Species Compatibility

TruSeq Custom Amplicon supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.

Inquire about Illumina Concierge services

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)

System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000

Species Category Other,Bovine,Mouse,Human,Rat

Specialized Sample Types FFPE

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Method Amplicon Sequencing,Custom Sequencing"

"Illumina/HiSeq SBS Kit V4 250 cycle kit/FC-401-4003/1 Ea

" Illumina 产品编号： FC-401-4003美  元  价： $10100.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

HiSeq SBS Kit v4 kits contain ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis (SBS) technology on enabled HiSeq sequencing systems* run in high output mode.

The HiSeq SBS v4 kits enable up to 1000 gigabases (Gb) of output per 6-day dual flow cell run using the HiSeq PE Cluster Kit v4 cBot. These kits contain sufficient volumes to support dual-indexing applications – no additional SBS reagents are required.

HiSeq v4 chemistry allows all reagents to be loaded onto the instrument before beginning the run. To perform a HiSeq v4 run, you need 1 HiSeq SBS Kit v4. Each kit includes sufficient reagents for sequencing 1 flow cell.

*HiSeq Systems require the latest hardware configuration. Check with your local sales rep regarding system compatibility.

Note: This kit is NOT compatIBLe with the Genome Analyzer.

FlexIBLe Run Configurations

You can choose from single-read or paired-end read runs, with read lengths up to 2×125 bp in high output mode. If longer read lengths are desired, rapid run SBS kits enable up to 2×250 bp.

A Broad Range of Applications

These kits enable a wide variety of applications, from ChIP-Seq to whole-genome sequencing, splice variant detection, and more. They enable researchers, in a single run, to process up to†:

10 genomes

156 exomes

80 whole-transcriptome RNA samples

†Assumes 100 Gb per genome at 2x125 bp, 5 Gb per exome at 2x100 bp with TruSeq Exome or TruSeq Rapid Exome kits, and 50 million reads per transcriptome.

This product is also available as an Illumina Advantage product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

Specifications:

Maximum Output Up to 1000 Gb per dual flow cell run

Maximum Reads per Run Up to 4 billion

System Compatibility HiSeq 2000,HiSeq 1500,HiSeq 2500

Reagent Type Sequencing by Synthesis

Technology Sequencing

"

"Illumina/Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)/FC-131-2001/1 Ea

" Illumina 产品编号： FC-131-2001美  元  价： $989.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids

Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time

Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.

Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications

Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Assay Time 90 minutes

Hands-On Time 15 minutes

Mechanism of Action Enzymatic Fragmentation

Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.

Input Quantity 1 ng DNA

Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria

System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode

Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants

Specialized Sample Types Low Input,Single Cells

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

"Illumina/HiSeq X Ten Reagent Kit v2.5 - 10 pack/FC-501-2521/1 Ea

" Illumina 产品编号： FC-501-2521美  元  价： $127500.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

HiSeq X Reagent Kits support high coverage whole-genome sequencing (WGS) with the HiSeq X Series of sequencing systems. By leveraging patterned flow cells, these kits provide increased cluster density to enable significant increases in data output and daily throughput. HiSeq X Reagent Kits support industry-leADIng library preparation options while offering superior data quality and coverage.

HiSeq X Reagent Kits support the TruSeq DNA PCR-Free Library Preparation Kit, for industry-best coverage of challenging genomic regions, and the TruSeq Nano DNA Library Preparation Kit, for efficient sequencing of samples with limited available DNA. Together, the reagent kits and library preparation options deliver exceptional genome coverage, enabling highly accurate and comprehensive variant calling for WGS on the HiSeq X Ten and the HiSeq X Five Systems. The HiSeq X Reagent Kits:

Deliver ultra-high data output

Provide ultra-high daily throughput

Enable sequencing up to 16 genomes per run

Are available in single and multipack configurations

Are compatIBLe with TruSeq DNA PCR-Free and TruSeq Nano DNA LibraryPreparation Kits

HiSeq X Reagent Kits are only compatIBLe with the HiSeq X Series of sequencing systems. HiSeq X Ten Reagent Kits are only available for purchase by HiSeq X Ten users and the HiSeq X Five Reagent Kits are only available for purchase by HiSeq X Five users. Reagents are also available in a multipack configuration to support bulk purchasing with significant reductions in packaging materials.

Latest technology innovation powers population-scale sequencing

HiSeq X Reagent Kits leverage the newest format in Illumina flow cell technology to achieve ultra-high-throughput WGS. Patterned flow cells contain billions of nanowell substrates at fixed locations across both surfaces of the flow cell. The structured organization (see below) provides even spacing of sequencing clusters to deliver significant increases in sequencing reads and total output of the system.

Specifications:

Maximum Output Up to 1800 Gb (1 HiSeq X System with dual flow cells)

Maximum Reads per Run Up to 6 billion

Reagent Type Paired-End Sequencing,Cluster Generation

System Compatibility HiSeq X Ten,HiSeq X Five

Technology Sequencing

"

"Illumina/HiSeq 3000/4000 SBS Kit (300 cycles)/FC-410-1003/1 Ea

" Illumina 产品编号： FC-410-1003美  元  价： $9900.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>簇生成和测序试剂公司分类： Cluster Generation & Sequencing Reagents "Product Highlights:

HiSeq 3000/4000 SBS (Sequencing by Synthesis) Kits support a wide range of sequencing applications, making them ideal for high-throughput labs performing various genomic applications. CompatIBLe with the HiSeq 3000 and HiSeq 4000 Sequencing Systems, the new kits leverage patterned flow cell technology for even cluster spacing and uniform feature size, to deliver extremely high cluster densities and significant increases in data output and daily throughput.

The HiSeq 3000/4000 SBS Kits enable up to 1500 Gb (1.5 Tb) of output per dual flow cell run when used with the HiSeq 3000/4000 PE Cluster Kit. This high capacity means that more samples can be sequenced simultaneously at a great depth, generating rich and meaningful data.

Specifications:

Maximum Output Up to 1500 Gb (HiSeq 4000 with 2 flow cells), Up to 750 Gb (HiSeq 3000)

Maximum Reads Up to 5 billion (HiSeq 4000), Up to 2.5 billion (HiSeq 3000)

Reagent Type Sequencing by Synthesis

System Compatibility HiSeq 3000,HiSeq 4000

Technology Sequencing"