- 面议
起订量：
CBP60380 MCF-7人乳腺癌细胞
- 型号
- CBP60380
该企业相似产品
南京康佰生物科技有限公司是一家专业提供，进口细胞，科研试剂，生命科学研究服务外包，生物大数据库的综合型公司。公司在多年的发展历程中，整合资源，悉心研发逐步建立起 "较大肿瘤细胞库”，"较大STR鉴定比对信息库”等专业平台，同时开发了分子生物学、蛋白工程、病毒包装、抗体工程、细胞工程、药物研发等一系列平台，与上海张江药谷、苏州纳米园、南京高新区及其他企业均建立了稳定的合作关系。
详细信息
CBP60380 |
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I. General information
Synonyms: | MCF 7; MCF.7; MCF7; Michigan Cancer Foundation-7; ssMCF-7; ssMCF7; MCF7/WT; IBMF-7; MCF7-CTRL |
Background: | Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen P2 containment. Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2. |
Species: | Homo sapiens, human |
Tissue: | Mammary gland, breast; derived from metastatic site: pleural effusion |
Disease: | Breast adenocarcinoma |
Gender: | Female, 69 years |
Morphology: | Epithelial-like cells growing as monolayers |
Growth Mode: | Adherent |
Doubling Time: | ca. 50 hours range 30-72 hours |
DNA Profile: | Amelogenin: X Cobioer’s Cell Line Authentication Service |
Culture Medium: | MEM + 10% FBS+ 1% Non Essential Amino Acids NEAA + 1mM Sodium Pyruvate NaP+ 10 µg/ml human insulininsulinoptional |
Cryopreservation medium: | 90% FBS+10%DMSO |
Photo: |
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Antigen Expression: | Blood Type O; Rh+ |
Receptor Expression: | Estrogen receptor, expressed |
Genes Expressed: | Insulin-like growth factor binding proteins IGFBP BP-2; BP-4; BP-5 |
Immunology: | cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - |
Molec. Genetics: | Expression of fusion gene BCAS4-BCAS3 confirmed by RT-PCR |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV - |
Comments: | The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene. |
