类器官Y-27632 dihydrochloride
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普迈精医科技(北京)有限公司(PRECISION MEDICINE TECHNOLOGY(Beijing)Co.LTD)是EVOQUA德国懿华在中国的合作伙伴,为实验室客户提供专业的科研检测设备解决方案。
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实验室纯水及超纯水供给解决方案:可根据客户需求,提供1.2-14升/分钟的超纯水,10-1000升/小时的纯水设备及设计方案,并提供全部相关耗材,EDI去离子模块。
生物样本库解决方案:从样本库前期设计、各功能区规划,到样本前、后处理设备、样本冻存耗材、样本冻存设备,再到样本自动化工作站、自动化样本库、样本库管理软件,为客户提供一站式样本存储解决方案。
细胞培养解决方案:该方案涵盖组织提取,研磨消解,培养分选,生化分析,分离富集,冻存转运各环节的仪器设备及耗材,并提供相关检测服务及科研服务。
细胞囊泡及外泌体EXOSOME解决方案:该方案涵盖细胞囊泡及外泌体EXOSOME的分离提取,鉴定鉴别,测序分析及后续应用。我们为客户提供相关环节专业仪器,试剂盒,耗材,并提供相关检测服务及科研服务。
分子生物学解决方案:该方案包含基因表达,分子克隆,蛋白表达,核酸检测等各分子生物学研究领域所需要的设备及耗材,并提供流程自动化方案和技术服务。
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类器官Y-27632 dihydrochloride
类器官(Organoids)是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致,从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出*的优势。
产品介绍DESCRIPTION
Background | Y-27632 dihydrochloride is an orally active, ATP-competitive inhibitor of ROCK-I and ROCK-II, with Kis of 220 and 300 nM, respectively. Y-27632 dihydrochloride attenuates Doxorubicin-induced apoptosis of human cardiac stem cells. Y-27632 also suppresses dissociation-induced apoptosis of murine prostate stem/progenitor cells. Y-27632 dihydrochloride primes human induced pluripotent stem cells (hIPSCs) to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation[1][2][3][4][5]. |
技术参数
M. W t | 320.26 | ||
Formula | C14H23Cl2N3O | ||
CAS No | 129830-38-2 | ||
Storage | Powder | -20 °C | 3 years |
In solvent | -80°C | 6 months | |
-20 °C | 1 month | ||
Solubility | DMSO | 33.33 mg/mL(104.07 mM; Need ultrasonic) | |
H2O | 100 mg/mL(312.25 mM; Need ultrasonic) |
BIOLOGICAL ALTIVITY
In Vitro
Y-27632 inhibits the ROCK family of kinases 100 times more potently than other kinases including protein kinase C, cAMP-dependent kinase and myosin light chain kinase. Y-27632 prolongs the lag time and delays the appearance of BrdU-labeled cells in a concentration-dependent manner, delays of about 1 and 4 h are noticed in the Swiss 3T3 cells treated with 10 and 100 μM Y-27632, respectively[1].
Y-27632 promotes neuronal differentiation of adipose tissue-derived stem cells (ADSCs). Compared to 1.0 and 2.5 μM Y-27632 induced groups, percentages of neuroal-like cells achieved a peak in the 5.0 μM Y-27632 induced group[2].
In Vivo
Y-27632 (5 and 10 mg/kg) significantly prolongs the onset time of myoclonic jerks when compare with saline group. Y-27632 (5 and 10 mg/kg) significantly prolongs the onset time of clonic convulsions when compare with saline group[3].
Treatment with Dimethylnitrosamine (DMN) causes a significant decrease in rat body and liver weight (DMN-S group) compared with control animals (S-S group). Oral Y27632 (30 mg/kg) essentially prevents this DMN-induced rat body and liver weight loss (DMN-Y group)[4].
类器官Y-27632 dihydrochloride