- 面议
起订量：
P2709 Protein G抗体纯化填料
- 型号
- P2709
上海维景生物科技有限公司是一家专注生命科学领域的企业，拥有强大的资金背景支持和专业的团队等资源。公司面向全国市场，迅速发展，广纳英才，现已建成专业生命科学产品销售团队，其中大部份是在生命科学市场上工作多年，有丰富的销售经验，也有良好的客户资源，销售渠道全面覆盖全国范围大部分工业以及科研客户，团队中免疫学及相关专业硕士以上学历人士达80%，有较强的技术支持能力，在针对专业技术服务中能够提供完善周到的专业化服务，对客户在使用产品过程中提出的问题能满意答复，并可及时传递较早的产品技术资料信息。
上海维景生物是一家面向全国以专业从事免疫学实验试剂和抗体试剂代理销售为主的公司，服务对象主要为大学研究所科研人员、大型制药公司等。
同时公司还是美国Engibody品牌的全国总代理，Engibody公司产品专注于标签抗体、内参抗体及标签抗体偶联beads这一领域。其开发的标签抗体偶联agarose beads（affinity gel）在科研以及临床研究中享有很高的声誉，更借助Engibody在标签抗体、内参抗体领域的优势，成为WB,IP,Co-IP全套试剂及试剂盒的专业供应商。
维景生物是一家专注标签抗体偶联beads（affinity gel），标签抗体，HRP直标标签抗体，内参抗体等抗体销售,及WB,IP,Co-IP全套试剂及试剂盒的高科技生物公司。
详细信息
产品名称：Protein G agarose Resin 6FF for Antibody Purification， Protein G抗体纯化填料
货号： P2709
品牌：Engibody
简介：Protein G抗体纯化填料，用于小鼠和大鼠的单克隆抗体及多克隆抗体的纯化。
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. P2709 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100µl to 0.5ml. Up to 500µg of antibody can be purified in each run.
The method involves capturing the antibody on the Protein G resin. Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. Once the antibody has bound to the Protein G, unwanted substances can be removed by simply washing the resin. Up to 500ug of antibody can be purified in each run.
Description
Protein G Resin for Antibody Purification
Tested applications
Antibody Purification
Form
50% slurry
Storage instruction
Store re-equilibrated Protein G Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
Beads Diameter
90 μm
Binding Capacity
>30 mg human IgG/mL settled resin
Ligand
Recombinant protein G produced in E.coli
Matrix
Highly cross-linked 6% agarose beads
Maximum pressure
0.3 MPa (3 bar)
Chemical stability
All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
Flow rate
Gravity flow, 100 - 300 cm/hour
pH stability
Long term: pH3 - 9;
Short term: pH2 - 10
Storage buffer
1x PBS containing 20% ethanol
市售抗体通常含有干扰酶或荧光团标记反应的物质（例如BSA、三嗪、叠氮化物）。P2709快速去除这些污染物。它还可以用于从诸如腹水或免疫血清的粗样品中纯化抗体。待纯化或净化的抗体的理想体积为100µl至0.5ml。每次运行最多可纯化500µg抗体。
该方法包括在蛋白G树脂上捕获抗体。蛋白质G对来自各种物种的IgG分子的Fc区具有高亲和力。一旦抗体与G蛋白结合，可以通过简单地清洗树脂来去除不需要的物质。每次运行最多可纯化500ug抗体。
描述
抗体纯化用G蛋白偶联beads
经过测试的应用实验
抗体纯化
类型
50%slurry
存储说明
将重新平衡的G蛋白树脂储存在2°C至8°C含20%乙醇的结合/洗涤缓冲液中。不要冻结。
beads直径
90微米
结合能力
>30 mg人IgG/mL纯beads
配体
大肠杆菌中产生的重组蛋白G
基质
高交联6%琼脂糖珠
最大压力
0.3 MPa
化学稳定性
所有常用的水性缓冲液，包括6M盐酸胍和8M尿素。
流速
重力流，100-300 cm/小时
pH稳定性
Long term: pH3 - 9;
Short term: pH2 - 10
存储缓冲液
1x PBS，含20%乙醇
Protein A and G are popular choices for antibody purification, because they are both stable and target selective. IgG class antibodies from multiple species bind to protein A and/or G, allowing antibody to be captured on protein-bound beads. Protein A and G bind IgG subtypes with varying affinities, determined by species and the properties of the heavy chain. This chart that reports the affinity of protein A and G for various species immunoglobulin subtypes.
