CALU抗原；钙腔蛋白(CALU)重组蛋白多种属
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详细信息
Recombinant Calumenin (CALU)
钙腔蛋白(CALU)重组蛋白
[ PROPERTIES ]
Residues: Lys20~Phe315
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O43852
Host: E. coli
Subcellular Location: Endoplasmic reticulumlumen. Secreted. Melanosome. Sarcoplasmicreticulum lumen.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 4.7
Predicted Molecular Mass: 38.8kDa
Accurate Molecular Mass: 49kDa as determined by SDS-PAGE reducingconditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
The sequence of the target protein is listed below.
K PTEKKDRVHH EPQLSDKVHN DAQSFDYDHD AFLGAEEAKT FDQLTPEESK ERLGKIVSKIDG DK DG F VT V DE LK DWIK FA QK RWIY E DV E RQWK G H D L NE DG LVSWE E YKNATYGYVLDD PDPDDGFNYK QMMVRDERRF KMADKDGDLI ATKEEFTAFL HPEEYDYMKDIVVQETMEDI DKNADGFIDL EEYIGDMYSH DGNTDEPEWV KTEREQFVEF RDKNRDGKMDKEETKDWILP SDYDHAEAEA RHLVYESDQN KDGKLTKEEI VDKYDLFVGS QATDFGEALVRHDEF
[ IDENTIFICATION ]
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