磷酸酶张力蛋白同源物(PTEN)重组蛋白
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详细信息
Recombinant Phosphatase And Tensin Homolog (PTEN)
磷酸酶张力蛋白同源物(PTEN)重组蛋白
[ PROPERTIES ]
Source:Prokaryotic expression
Host: E.coli
Residues: Thr2~Val403
Tags:N-terminal His Tag
Subcellular Location: Nucleus, Secreted, Cytoplasm
Purity: > 90%
Traits:Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Original Concentration:600µg/mL
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point:6.1
Predicted Molecular Mass: 48.6kDa
Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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