- ¥58871起订
起订量：
ROCHE 03703126001- Annexin-V-Alexa 568
- 型号
- ROCHE 03703126001-
上海叶舟生物科技有限公司从成立之初就秉着服务科研 , 传承文明的理念 , 努力为中国广大生命科学工作者提供Z全面的产品与信息咨询 。 传播科技文明 , 服务人类健康一直都是生命科学研究人员奋斗的目标 , 在这个文明的梯队里 , 我们能为广大科研工作者提供一些便捷的服务 , 使他们能全身心的投入工作 , 摆脱一些琐事的烦恼 , 把较大的能量释放到Z前沿的工作中去 , 为此我们感到十分的欣慰。 浩然生物始终坚持社会效应与公司发展的双重奉献 , 严格稳定的财务政策确保了我们向科技工作者提供产品与服务的持续性 , 同时浩然生物也和广大科研工作人员一样努力的工作 , 在品牌不断延伸与壮大的同时时刻警醒自己知恩图报回馈社会的责任。 缩小文明差距 , 扩大科技交流一直是我们的工作指向 , 为此 , 我们时刻关注着世界生命科学的较早动态 , 并努力打造一个相互交流的平台 , 把优秀的民族产品介绍给世界 , 把 *的技术与资讯带回中国 , 文明的平衡与和谐是我们永远追求的目标。同时我们要特别感谢在公司发展的过程当中给过我们很多建议与意见的中国当代的科技工作者，是他们使我们感到了公司必须尽快发展的责任与压力，我们也要感谢在公司发展过程当中给过我们很多竞争压力与困难的国内外的生物公司，是他们使我们感到了公司必须居安思危逆流而上，时刻保持创新的思想与大量的行动，努力增强抗击打与出奇制胜的能力。上海浩然生物技术有限公司随时欢迎与期待着各位用户与朋友的批评与建议。
详细信息
产品名称：Annexin-V-Alexa 568
产品货号：
产品包装：500 µl 250 tests
产品价格：2087
产品应用：The analysis of phosphatidylserine on the outer leaflet of apoptotic cell membranes is performed using Annexin-V-Alexa 568 in combination with a DNA stain （e.g., BOBO-1） to differentiate apoptotic cells from necrotic cells. Apoptotic and necrotic cells can also be differentiated by cell surface markers.
产品优势：Detection flexibility:Analysis of results with flow cytometry and/or fluorescence microscopy
Alexa is a red dye, which can be used for double or triple staining with other fluorescent markers （e.g., fluorescein-labeled surface markers）
Accuracy:Accuray distinguish apoptotic cells from necrotic cells
Stability of results:High photostability （long observation possible）
Rapid and simple procedure
产品介绍：Alexa 568 is a fluorescent label. The Annexin-V-Alexa 568 conjugate can be easily analyzed on a 488-nm laser Fluorescent Analytical Cell Sorter （FACS） machine, or using a fluorescence microscope. Excitation is in the range of 488 - 596 nm with emission above 600 nm.
Additional secondary labeling is also possible. For example, membrane surfaces can be stained with fluorescein-labeled monoclonal antibodies for further cellular characterization.
背景：In the early stages of apoptosis, changes occur on the cell surface. One of these plasma membrane alterations is the translocation of phosphatidylserine （PS） from the inner side of the plasma membrane to the outer layer, thereby exposing PS at the external surface of the cell. Fadok et al. showed that macrophages specifically recognize PS exposed on the surface of lymphocytes during apoptosis. This recognition and subsequent phagocytosis of apoptotic cells and bodies protects organisms from exposure to cellular compounds that lead to inflammation and usually accompany necrosis.
Annexin-V is a Ca2+-dependent, phospholipid-binding protein with a high affinity for PS. This protein can therefore be used as a sensitive probe for PS exposure on the outer leaflet of the cell membrane, and is suited to detect apoptotic cells. Since necrotic cells also expose PS as a result of lost membrane integrity, apoptotic cells must be differentiated from these necrotic cells. The simultaneous application of a DNA stain （used for dye-exclusion tests [e.g., BOBO-1]） allows the discrimination of necrotic cells from the Annexin-V positively stained cell cluster.
产品组成：500 µl, ready-to-use solution, for 250 tests
