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Roche11480863001 DIG Oligonucleotide 5'-End Labeling Set

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Roche11480863001
广州市齐云生物技术有限公司

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详细信息

DIG Oligonucleotide 5'-End Labeling Set

Cat.No. Pack Size Qty Price Options
11480863001 1 set for at least 10 labeling reactions 6115

Application

The set is used for 5'-end labeling of oligonucleotides with digoxigenin. The oligonucleotide is reacted in a final synthesis step with an aminolinker phosphoramidite, creating a 5'-terminal amino function. After completion of the oligonucleotide synthesis, the digoxigenin moiety is introduced at the 5' position.
In general, 1 mg NHS-ester is sufficient for the labeling of approximay 300 nmol of amino-substituted oligonucleotide.
 


Figure 1: Typical elution profile of a digoxigenin-labeled oligonucleotide.

Product Description

Stability: The compounds of the set are stable in solid form for at least one year when stored at –15 to –25°C. The phosphoramidite solution is stable for approximay two weeks at ambient temperature with the exclusion of moisture. The solution of the NHS-ester in ethanol is stable only for a short time. Therefore, the ester solution should be used for several parallel labelings.

Contents

  1. “Aminolinker” [N-Trifluoroacetamido-(3-oxa)-pentyl-N,N-diisopropyl-methyl]–phosphoramidite, 100 mg
  2. Digoxigenin-NHS-ester, (Digoxigenin-3-0-methylcarbonyl-e-aminocaproic acid-N-hydroxy-succinimide ester), 5 x 1 mg

 

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