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Roche11480863001 DIG Oligonucleotide 5'-End Labeling Set
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代理商Cat.No. | Pack Size | Qty | Price | Options |
---|---|---|---|---|
11480863001 | 1 set for at least 10 labeling reactions | 6115 |
The set is used for 5'-end labeling of oligonucleotides with digoxigenin. The oligonucleotide is reacted in a final synthesis step with an aminolinker phosphoramidite, creating a 5'-terminal amino function. After completion of the oligonucleotide synthesis, the digoxigenin moiety is introduced at the 5' position.
In general, 1 mg NHS-ester is sufficient for the labeling of approximay 300 nmol of amino-substituted oligonucleotide.
Figure 1: Typical elution profile of a digoxigenin-labeled oligonucleotide.