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Roche11636111910 PCR ELISA, DIG-Detection
- 型号
- Roche11636111910
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详细信息
PCR ELISA, DIG-Detection
| For semi-quantitative detection of DIG-labeled products by a hybridization-based microplate assay. |
| Cat.No. | Pack Size | Qty | Price | Options |
|---|---|---|---|---|
| 11636111910 | 1 kit for 192 detection reactions （two microplates） allows the semi-quantitative detection of 50 PCR products |
Application
Use the PCR ELISA, DIG-Detection kit to detect DIG-labeled PCR products.
Note: The PCR ELISA system provides a convenient, nonradioactive method for labeling and detecting PCR products. It is approx. 100 times more sensitive than conventional ethidium bromide staining of products on agarose gels. （See "Principle" below for an overview of the PCR ELISA technique.）
Product Description
Brief description: The PCR ELISA, DIG-Detection kit provides all the reagents （except biotin-labeled capture probes specific for your target DNA） needed for detecting DIG-labeled PCR products. It also provides a control PCR product and control capture probe to ensure the detection process is working correctly.
Labeling: The DIG-labeled PCR products detected with this kit contain alkali-stable digoxigenin-11-dUTP （DIG-dUTP）. Such DIG-labeled products can be prepared with the PCR ELISA, DIG-Labeling kit.
Capacity: This size of the kit allows semi-quantitative detection of approx. 50 DIG-labeled PCR products. This number will vary depending upon the number of sample dilutions, controls, and/or standards used in the assay
Contents
- Microplate Modules （precoated with streptavidin and postcoated with blocking reagent）, 24 x 8 wells
- Strip Frames to hold Microplate Modules, 2
- Cover Foils for Microplate Modules, 8
- Anti-Digoxigenin-Peroxidase （enzyme-conjugated antibody, anti-DIG-POD）, 2 x 250 mU
- ABTS Substrate, 20 tablets
- Substrate Buffer, 125 ml
- Conjugate Dilution Buffer, 60 ml
- Washing Buffer Tablets （for preparation of plate wash liquid）, 5
- Hybridization Buffer, 60 ml
- Denaturation Solution, 4 x 1.1 ml
- Control Capture Probe, biotin-labeled, 75 pmol
- Control PCR Product, DIG-labeled, enough for 5 control detection procedures
Principle
Notes
This kit is part of a complete PCR ELISA system. If you combine it with other reagents and equipment, you can adapt the system for many molecular biology applications. For example:
- If you have an appropriate target-specific, biotin-labeled capture probe, you can distinguish PCR products that differ by as little as a single base pair. Thus the PCR ELISA system （DIG-labeling + DIG-detection） can be used:
- to detect point mutations, deletions or insertions.
- to classify target sequences （e.g., as in HLA-typing or cell typing）.
- Preparation of suitable DIG-labeled standards and addition of a colorimetric detection system will allow you to quantify PCR products.
- The microplate format used by the PCR ELISA system makes the system compatible with automated plate preparation and reading systems.
