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Roche 11667327001 DNA Isolation Kit for Mammalia;Roche 11667327001原装
- 型号
- Roche 11667327001
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详细信息
产地：Roche
货号：11667327001
Benefits
Process multiple samples simultaneously.
DNA is easily purified in less than 90 minutes.
Use a cost-effective kit.
The convenient kit purifies DNA for the cost of most homebrew methods.
Obtain consistent and reliable results.
The kit enables analysis of different sample volumes （1 to 10 ml） with varying amounts of leukocytes.
Product Description
Sample
Human whole blood （treated with sodium heparin, EDTA or sodium citrate）: 1 - 10 ml
Isolated lymphocytes: 1 - 10 ml
Isolated buffy coat: buffy coat from 10 - 20 ml whole blood, containing at least 1.0 x 107 leukocytes
Application
The DNA Isolation Kit for Mammalian Blood is designed for the rapid isolation of DNA from 1-10 ml mammalian whole blood, lymphocyte, or buffy coat samples. The purified DNA is suitable for multiple applications, including standard PCR, long-range PCR, sequencing, and Southern blots. The entire procedure can be completed in less than 1.5 hours （plus 30-60 minutes resuspension time）, requiring no organic extractions or column-purification steps. Multiple samples can be easily processed. .
Time required
The entire procedure can be completed in less than 1.5 hours.
Purity of isolated DNA
Average A260 / A280 ratio: 1.7 - 1.9
Contents
Red Blood Cell Lysis Buffer, 750 ml
White Blood Cell Lysis Buffer, 125 ml
Protein Precipitation Solution, 65 ml
Principle
The DNA Isolation Kit for Mammalian Blood procedure relies on the separation of white blood cells from whole blood via a preferential red blood cell lysis. In the presence of a strong anionic detergent, the white blood cells are lysed, then the proteins are removed by dehydration and precipitation. The purified DNA is subsequently recovered via ethanol precipitation.
