- ¥28001起订
起订量：
070222h Human Interferon,Beta（IFNb） ELISA Kit
- 型号
- 070222h
该企业相似产品
上海武昊经贸有限公司, 是一家专业经营生命科学领域的科研试剂(如：免疫组化抗体;流式产品-Foxp3,Treg-调节性T细胞检测试剂盒；酶联免疫(ELISA)试剂盒等)、仪器(高效液相色谱，色谱球，色谱塔，示波器，频谱分析仪等)、实验室耗材(如：CORNING-细胞培养板，培养瓶，培养皿，离心管等),水中微生物检测试剂的企业。
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详细信息
一、人β干扰素 ELISA试剂盒的产品简述：
ELISA 品 牌： USCN
ELISA 货 号：070222h
ELISA英文名：human IFNb ELISAKit
ELISA中文名：人β干扰素,IFNb ELISA 试剂盒
二、人IFNb ELISAKIT的技术参数：
1. 检测范围：
2. 灵 敏 度：
3. 适用标本：
4.保存条件：2-8℃
5. 有 效 期： 6个月
三、ELISA试剂盒的产品用途：
产品用途：酶联免疫实验（ELISA实验）
备 注：仅供参考，请以随货纸质说明书为准，更多产品如抗体（antibody）；蛋白（protein）；鲎试剂；内毒素工作标准品；内毒素指示剂；无热原检查用水；无热原吸咀；紫外灯；色谱仪；色谱柱等更详细信息请登录上海武昊公司www.wuhaosh.com 或致电咨询
ELISA实验前，血清、血浆、组织、细胞培养上清等样品的采集及处理
Serum - Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4oC before centrifugation for 20 minutes at approximay 1000×g. Assay freshly prepared serum immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 000×g at 2 - 8oC within 30 minutes of collection. Remove plasma and assay immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS（0.02mol/L,pH 7.0-7.2） to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in 5-10 mL of PBS with a glass homogenizer on ice（Micro Tissue Grinders woks, too）. The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 5 minutes at 5000×g. Remove the supernate and assay immediay or aliquot and store at ≤-20oC.
Cell culture supernates - Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
