实验开始前,请提前配置好所有试剂,试剂或样品稀释时,均需混匀,混匀时尽量避免起泡。每次检测都应该做标准曲线。如样品浓度过高时,用样品稀释液进行稀释,以使样品符合试剂盒的检测范围。
1. 加样:分别设空白孔、标准孔、待测样品孔。空白孔加样品稀释液100μl,余孔分别加标准品或待测样品100μl,注意不要有气泡,加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀,酶标板加上盖或覆膜,37℃反应120分钟。
2. 弃去液体,甩干,不用洗涤。每孔加生物素标记抗体工作液 100μl(取1μl生物素标记抗体加99μl生物素标记抗体稀释液的比例配制,轻轻混匀,在使用前一小时内配制),37℃,60分钟。
3. 温育60分钟后,弃去孔内液体,甩干,洗板3次,每次浸泡1-2分钟,350μl/每孔,甩干。
4. 每孔加辣根过氧化物酶标记亲和素工作液(同生物素标记抗体工作液) 100μl,37℃,60分钟。
5. 温育60分钟后,弃去孔内液体,甩干,洗板5次,每次浸泡1-2分钟,350μl/每孔,甩干。
6. 依序每孔加底物溶液90μl,37℃避光显色(30分钟内,此时肉眼可见标准品的前3-4孔有明显的梯度蓝色,后3-4孔梯度不明显,即可终止)。
7. 依序每孔加终止溶液50μl,终止反应(此时蓝色立转黄色)。终止液的加入顺序应尽量与底物液的加入顺序相同。为了保证实验结果的准确性,底物反应时间到后应尽快加入终止液。
8. 用酶联仪在450nm波长依序测量各孔的光密度(OD值)。 在加终止液后15分钟以内进行检测。
FS-e10001 人原钙黏素1(PCDH1)ELISA试剂盒 Human protocadherin 1,PCDH1ELISA试剂盒
FS-e10002 人白介素2受体(IL-2R)ELISA试剂盒 Human Interleukin-2 receptor,IL-2RELISA试剂盒
FS-e10003 人皮肤T细胞虏获趋化因子(CTACK/CCL27)ELISA试剂盒 Human cutaneous T cell-attracting chemokine,CTACKELISA试剂盒
FS-e10004 人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒 Human Breast and kidney expressed chemokine,BRAKELISA试剂盒
FS-e10005 人B-淋巴细胞趋化因子1(BLC-1/CXCL13)ELISA试剂盒 Human B-Lymphocyte Chemoattractant 1,BLC-1ELISA试剂盒
FS-e10006 人结缔组织活化肽Ⅲ(CTAPⅢ)ELISA试剂盒 Human connective tissue-activating peptide Ⅲ,CTAPⅢELISA试剂盒
FS-e10007 人巨噬细胞集落刺激因子受体(M-CSFR)ELISA试剂盒 Human Macrophage Colony-Stimulating Factor Receptor,M-CSFRELISA试剂盒
FS-e10008 人免疫球蛋白AFc段受体Ⅰ(FcαRⅠ/CD89)ELISA试剂盒 Human Receptor Ⅰ for the Fc region of immunoglobulin A,FcαRⅠELISA试剂盒
FS-e10009 人免疫人低氧诱导因子1α(HIF-1α)ELISA试剂盒球蛋白EFc段受体Ⅱ(FcεRⅡ/CD23)ELISA试剂盒 Human Receptor Ⅱ for the Fc region of immunoglobulin E,FcεRⅡELISA试剂盒
FS-e10010 人免疫球蛋白GFc段受体Ⅲ(FcγRⅢ/CD16)ELISA试剂盒 Human Receptor Ⅲ for the Fc region of immunoglobulin G,FcγRⅢELISA试剂盒
FS-e10011 人免疫球蛋白GFc段受体Ⅱ(FcγRⅡ/CD32)ELISA试剂盒 Human Receptor Ⅱ for the Fc region of immunoglobulin G,FcγRⅡELISA试剂盒
FS-e10012 人免疫球蛋白GFc段受体Ⅰ(FcγRⅠ/CD64)GFc段受体Ⅰ(FcγRⅠ/CD64)ELISA试剂盒 Human Receptor Ⅰ for the Fc region of immunoglobulin G,FcγRⅠELISA试剂盒
FS-e10014 人免疫球蛋白GFc段受体Ⅱ(FcγRⅡ/CD32)ELISA试剂盒 Human Receptor Ⅱ for the Fc region of immunoglobulin G,FcγRⅡELISA试剂盒
FS-e10015 人免疫球蛋白GFc段受体Ⅰ(FcγRⅠ/CD64)ELISA试剂盒 Human ReceptorⅠfor the Fc region of immunoglobulin G,FcγRⅠ ELISA试剂盒
FS-e10016 人粒细胞趋化蛋白-2(GCP-2/CXCL6)ELISA试剂盒 Human granulocyte chemotactic protein-2,GCP-2 ELISA试剂盒
FS-e10017 人ω干扰素(IFN-ω)ELISA试剂盒 Human Interferon ω,IFN-ωELISA试剂盒
FS-e10018 人糖基化依赖的细胞黏附分子(GlyCAM-1)ELISA试剂盒 Human glycosylation-dependent cell adhesion molecule-1,GlyCAM-1ELISA试剂盒
FS-e10019 人干扰素调节因子(IRF)ELISA试剂盒 Human interferon Regulatory Factor,IRFELISA试剂盒
FS-e10020 人淋巴毒素β(LTB)ELISA试剂盒 Human lymphotoxinβ,LTBELISA试剂盒
FS-e10021 人淋巴毒素α(LTA)ELISA试剂盒 Human lymphotoxinα,LTAELISA试剂盒
FS-e10022 人CC趋化因子受体1(CCR1)ELISA试剂盒 Human CC-chemokine receptor 1,CCR1ELISA试剂盒
FS-e10023 人CX3C趋化因子受体1(CX3CR1)ELISA试剂盒 Human CX3C-chemokine receptor 1,CX3CR1ELISA试剂盒
FS-e10024 人肺部活化调节趋化因子(PARC/CCL18)ELISA试剂盒 Human pulmonary activation regulated chemokine,PARCELISA试剂盒
FS-e10025 人黏膜地址素细胞黏附分子(MAdCAM-1)ELISA试剂盒 Human mucosal addressin cell adhesion molecule-1,MAdCAM-1ELISA试剂盒
FS-e10026 人β干扰素(IFN-β/IFNB)ELISA试剂盒 Human Interferon β,IFN-β/IFNB ELISA试剂盒
FS-e10027 人可溶性CD38(sCD38)ELISA试剂盒 Human Soluble Cluster of differentiation 38,sCD38ELISA试剂盒
FS-e10028 人可溶性CD21(CR2/sCD21)ELISA试剂盒 Human Soluble Cluster of differentiation 21,sCD21ELISA试剂盒
FS-e10029 人可溶性瘦素受体(sLR)ELISA试剂盒 Human Leptin Soluble Receptor,sLRELISA试剂盒
FS-e10030 人Toll样受体9(TLR-9/CD289)ELISA试剂盒 Human Toll-like receptor 9,TLR-9ELISA试剂盒
FS-e10031 人转化生长因子β2(TGFβ2)ELISA试剂盒 Human transforming growth factors β2,TGFβ2 ELISA试剂盒
FS-e10032 人单核细胞趋化蛋白4(MCP-4/CCL13)ELISA试剂盒 Human monocyte chemotactic protein 4,MCP-4 ELISA试剂盒
FS-e10033 人白三烯D4(LTD4)ELISA试剂盒 Human leukotriene D4,LT-D4ELISA试剂盒
FS-e10034 人N钙黏蛋白/神经钙黏蛋白(N-Cad)ELISA试剂盒 Human Neural-Cadherin, N-CadELISA试剂盒
FS-e10035 人肝素结合性表皮生长因子(HB-EGF)ELISA试剂盒 Human heparin-binding epidermal growth factor-like growth factor,HB-EGFELISA试剂盒
FS-e10036 人红细胞刺激因子(ESF)ELISA试剂盒 Human erythropoiesis stimulating factor,ESFELISA试剂盒
FS-e10037 人肿瘤坏死因子相关激活诱导因子(TRANCE)ELISA试剂盒 Human TNF related activation induced cytokine,TRANCEELISA试剂盒
FS-e10038 人生长激素释放因子(GH-RF)ELISA试剂盒 Human growth hormone relasing factor,GH-RFELISA试剂盒
FS-e10039 人巨噬细胞趋化因子(MCF)ELISA试剂盒 Human macrophage chemotatic factor,MCFELISA试剂盒
FS-e10040 人α/β干扰素受体(IFN-α/βR)ELISA试剂盒 Human Interferon α/βReceptor,IFN-α/βRELISA试剂盒
FS-e10041 人B细胞生长因子(BCGF)ELISA试剂盒 Human B cell growth protein,BCGFELISA试剂盒
FS-e10042 人B细胞分化因子(BCDF)ELISA试剂盒 Human B cell differetiation factor,BCDFELISA试剂盒
FS-e10043 人上皮细胞粘附分子(Ep-CAM/CD362)ELISA试剂盒 Human Epithelial Cell Adhesion Molecule,Ep-CAMELISA试剂盒
FS-e10044 人可溶性粘附分子(Sam)ELISA试剂盒 Human soluble adhesion molecules,SamELISA试剂盒
FS-e10045 人巨噬细胞替代激活相关化学因子1(AmAC-1)ELISA试剂盒 Human Alternative macrophage activation-associated CC chemokine 1,AmAC-1ELISA试剂盒
FS-e10046 人可溶性血管内皮生长因子受体2(VEGFR-2/sFLK-1)ELISA试剂盒 Human soluble vascular endothelial growth factor receptor-2,VEGFR-2/sFLK-1ELISA试剂盒
FS-e10047 人胸腺基质淋巴细胞生成素(TSLP)ELISA试剂盒 Human thymic stromal lymphopoietin,TSLPELISA试剂盒
FS-e10048 人穿孔素/成孔蛋白(PF/PFP)ELISA试剂盒 Human Perforin/Pore-forming protein,PF/PFPELISA试剂盒
FS-e10049 人多效生长因子(PTN)ELISA试剂盒 Human pleiotrophin,PTNELISA试剂盒
FS-e10050 人可溶性CD28(sCD28)ELISA试剂盒 human soluble cluster of differentiation 28,sCD28 ELISA试剂盒
FS-e10051 人淋巴细胞因子ELISA试剂盒 Human lymphocyte factorELISA试剂盒
FS-e10052 人胸腺活化调节趋化因子(TARC/CCL17)ELISA试剂盒 Human thymus activation regulated chemokine,TARCELISA试剂盒
FS-e10053 人神经细胞粘附分子配体1(NCAM-L1/CD171)ELISA试剂盒 Human Neural cell adhesion molecule ligand 1,NCAM-L1ELISA试剂盒
FS-e10054 人神经保护因子(CVNPF)ELISA试剂盒 Human Cobra venom neuronal protective factor,CVNPFELISA试剂盒
FS-e10055 人可溶性肿瘤坏死因子α受体(sTNFαR)ELISA试剂盒 Human soluble Tumor Necrosis Factorαreceptor,sTNFαRELISA试剂盒
FS-e10056 人可溶性细胞因子受体(sCKR)ELISA试剂盒 Human soluble cytokine receptor,sCKRELISA试剂盒
FS-e10057 人可溶性凋亡相关因子配体(sFASL)ELISA试剂盒 Human soluble Factor-related Apoptosis ligand,sFASL/Apo-1ELISA试剂盒
FS-e10058 人细胞凋亡抑制因子(IAP)ELISA试剂盒 Human inhibitor of apoptosis,IAPELISA试剂盒
FS-e10059 人集落刺激因子(CSF)ELISA试剂盒 Human colony-stimulating factor,CSFELISA试剂盒
FS-e10060 人γ干扰素诱导单核细胞因子(MIGF/CXCL9)ELISA试剂盒 Human monocyte interferon gamma inducing factor,MIGFELISA试剂盒
FS-e10061 人干扰素诱导T细胞趋化因子(ITAC/CXCL11)ELISA试剂盒 Human Interferon inducible T-cell Chemoattractant,I-TACELISA试剂盒
FS-e10062 人CD14分子(CDl4)ELISA试剂盒 Human cluster Of differentiation,CDl4ELISA试剂盒
FS-e10063 人凋亡诱导因子(AIF)ELISA试剂盒 Human apoptosis inducing factor,AIFELISA试剂盒
FS-e10064 人白细胞共同抗原(LCA/CD45)ELISA试剂盒 Human leukocyte common antigen,LCA/CD45ELISA试剂盒
FS-e10065 人CD4分子(CD4)ELISA试剂盒 Human cluster Of differentiation,CD4ELISA试剂盒
FS-e10066 人P钙黏蛋白/胎盘钙黏蛋白(P-cad)ELISA试剂盒 Human Placenta Cadherin,P-cadELISA试剂盒
FS-e10067 人角化细胞生长因子(KGF)ELISA试剂盒 Human Keratinocyte Growth Factor,KGFELISA试剂盒
FS-e10068 人血小板衍生生长因子BB(PDGF-BB)ELISA试剂盒 Human Plaet-Derived Growth Factor-BB,PDGF-BB ELISA试剂盒
FS-e10069 人CXC趋化因子配体16(CXCL16)ELISA试剂盒 Human CXC-chemokine ligand 16,CXCL16ELISA试剂盒
FS-e10070 人CXC趋化因子受体3(CXCR3)ELISA试剂盒 Human CXC-chemokine receptor 3,CXCR3ELISA试剂盒
FS-e10071 人γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒 Human interferon-inducible protein 16,IFI16/p16ELISA试剂盒
FS-e10072 人基质细胞衍生因子1a(SDF-1a/CXCL12)ELISA试剂盒 Human Stromal cell derived factor 1a,SDF-1aELISA试剂盒
FS-e10073 人淋巴细胞趋化因子(Lptn/LTN/XCL1)ELISA试剂盒 Human lymphotactin,Lptn/LTNELISA试剂盒
FS-e10074 人α干扰素(IFN-α)ELISA试剂盒 Human Interferon α,IFN-αELISA试剂盒
FS-e10075 人可溶性CD86(B7-2/sCD86)ELISA试剂盒 Human soluble Cluster of differentiation 86,sCD86ELISA试剂盒