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Ficoll-Paque PREMIUM

时间:2017-04-06      阅读:2505

Ficoll-Paque PREMIUM 

  • 17-5442-02  6X100ml 1.078 g/ml

Ficoll-Paque PREMIUM产品系列为无菌即用型密度梯度介质,用于制备单核细胞。

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2012.
  • Low levels of endotoxin (< 0.12 EU/ml) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for products.

products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2012.

Applications


Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

1.084

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

1.073

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

ApplicationOptimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
Endotoxin Activity Max.< 0.12 EU/ml
Properties products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from blood and bone marrow
Estimated Shelf Life from Manufacture Date3 years
Storage Conditions4–30°C
Stability products are provided as sterile solutions which are stable for at least 3 yr when stored between 4°C and 30°C and protected from light. Deterioration of products is indicated by the appearance of a yellow color or particulate material in the solution
Physical StateLiquid
Osmolality Max.310 mOsm/kg
pH Range5.5–7.5
ColorColorless to slightly yellow
Certificate of AnalysisYes
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