天狼猩红染液
时间：2012-11-22 阅读：434
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海德创业（北京）生物科技有限公司 -
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0.1%的天狼猩红溶于饱和苦味酸溶液
海德创业（北京）生物科技有限公司
This method eliminates cytoplasmic staining, revealing thin septa and collagen fibers clearly.
Fixation
Bouins' Fixative
Sections
Parrafin @ 7 microns.
Staining
1. Deparaffinize and hydrate to distilled water.
2. Wash in running tap water, 10 minutes.
3. Rinse in distilled water.
4. Treat in Phosphomolybdic Acid 0.2% Aqueous （1-5 minutes）
5. Stain in Sirius Red, 0.1% in Saturated Picric Acid , for 90 minutes.
6. Wash for 2 minutes in 0.01 N Hydrochloric Acid .
7. Rinse in 70% Alcohol for 45 seconds.
8. Dehydrate, clear and mount in Permount
Results
| Septa | Red |