Perfectstart SYBR Green qPCR master mi  Taq PCR预混液

TQ2300-02Perfectstart SYBR Green qPCR master mi Taq PCR预混液

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2015-11-15 11:13:09
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上海索宝生物科技有限公司

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产品简介

Perfectstart SYBR Green qPCR master mix
热启动SYBR Green 实时定量PCR 预混液

本产品是采用PCR技术结合SYBR Green 嵌合荧光法进行实时荧光定量PCR 的试剂

年终特销 有意者速来2389454263或相应 欢迎光临!

详细介绍

Perfectstart SYBR Green qPCR master mix
热启动SYBR Green 实时定量PCR 预混液
 
本产品是采用PCR技术结合SYBR Green 嵌合荧光法进行实时荧光定量PCR 的试剂,产品中含有已优化浓度的Perfectstart DNA聚合酶、Buffer、dNTP和SYBR Green,使用时只需加入模板和引物即可进行Real-Time PCR 实验, 操作方便、快捷,并且重复性好。本产品适合现有市场上绝大部分Real-Time PCR 仪,产品附带的ROX Dye, 可进行反应校正。基于SYBR Green 法检测的定量PCR反应Mix ,采用热启动的Taq酶进行扩增,能有效避免非特异性扩增及引物二聚体产生,优化的反应体系,能高效的进行低拷贝数基因扩增 ,扩增效率高,能在宽广的范围内进行定量,采用2X Mix包装,使用方便快捷。可应用于各种DNA或者cDNA的实时定量PCR或者两步法RT-PCR等.此产品4°C避光保存,可在-20°C 长期保存,一旦融解请于4°C 保存。反复冻融制品性能可能下降,请避免多次反复冻融制品。
 
 
 
定购信息
编码 名称 次数 详情 单价
TQ2300-01 Perfectstart SYBR Green qPCR master mix 1.25ml Perfectstart SYBR Green qPCR master mix 600.00
TQ2300-02 Perfectstart SYBR Green qPCR master mix 1.25mlX4 Perfectstart SYBR Green qPCR master mix 2100.00
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PCR与RT-PCR相关  
Taq DNA Polymerase:DNA聚合酶  
TQ2100-00 Taq DNA Polymerase(500U) 160
TQ2100-01 Taq DNA Polymerase(1000U) 300
TQ2100-02 Taq DNA Polymerase(5000U) 1300
TQ2100-03 Taq DNA Polymerase(10000U) 2200
PCR Amplification Kit  
TQ2104-01 PCR Amplification Kit(500U) 250
TQ2104-02 PCR Amplification Kit(1000U) 480
TQ2104-02 PCR Amplification Kit(5000U) 2300
Pfu DNA polymerase:pfu DNA聚合酶  
TQ2101-00 Pfu DNA Polymerase(250U) 180
TQ2101-01 Pfu DNA Polymerase(500U) 350
Perfectstart Taq DNA polymerase:热启动Taq DNA聚合酶  
TQ2102-00 Perfectstart Taq DNA polymerase(500U) 800
TQ2102-01 Perfectstart Taq DNA polymerase(1000U) 1500
Taq plus DNA polymerase:Taq plus DNA聚合酶  
TQ2103-00 Taq Plus DNA Polymerase(500U) 250
TQ2103-01 Taq Plus DNA Polymerase(1000U) 480
Taq master mix:Taq PCR预混液  
TQ2201-01 2 X Taq MasterMixture(5x1ml)含染料 500
TQ2201-02 2 X Taq MasterMixture(20x1ml)含染料 1800
TQ2201-03 2 X Taq MasterMixture(50x1ml)含染料 4250
TQ2200-01 2 X Taq MasterMixture(5x1ml)不含染料 450
TQ2200-02 2 X Taq MasterMixture(20x1ml)不含染料 1600
TQ2200-03 2 X Taq MasterMixture(50x1ml)不含染料 4000
Perfectstart Taq master mix:热启动Taq PCR预混液  
TQ2202-01 Perfectstart Taq master 2X Mix(5x1ml)  1450
TQ2202-02 Perfectstart Taq master 2X Mix(10x1ml)  2600
High-Fidelity PCR master mix:高保真PCR预混液  
TQ2203-01 High-Fidelity PCR master 2X Mix(5x1ml) 700
TQ2203-02 High-Fidelity PCR master 2X Mix(20x1ml) 1200
Perfectstart SYBY Green qPCR master mix:热启动SYBR Green 实时定量PCR预混液
TQ2300-01 Perfectstart SYBR Green qPCR master mix 600
TQ2300-02 Perfectstart SYBR Green qPCR master mix 2100
Perfectstart Taqman qPCR master mix:热启动Taqman实时定量PCR预混液  
TQ2301-01 Perfectstart Taqman qPCR master mix 600
TQ2301-02 Perfectstart Taqman qPCR master mix 2100
M-MLV transcriptase:M-MLV逆转录酶  
TQ2401-01 M-MLV trancriptase(5000U) 380
TQ2401-02 M-MLV trancriptase(50000U) 3500
M-MLV first strand cDNA Synthesis Kit:M-MLV*链cDNA合成试剂盒  
TQ2501-01 M-MLV first strand cDNA Synthesis Kit(20) 480
TQ2501-02 M-MLV first strand cDNA Synthesis Kit(100) 2200
One Step RT-PCR kit:一步法反转录PCR试剂盒  
TQ2601-01 Omega One step RT-PCR kit(25) 700
TQ2601-02 Omega One step RT-PCR kit(100) 2700
10mM dNTPs    
TQAC135 dNTP solution,10mM(4x250ul) 170
TQAC136 dNTP solution,25mM(4x250ul) 300
100mM dNTP each    
TQAC131 dATP solution,100mM(250ul) 200
TQAC132 dcTP solution,100mM(250ul) 200
TQAC133 dGTP solution,100mM(250ul) 200
TQAC134 dTTP solution,100mM(250ul) 200
动物组织直接PCR试剂盒:动物组织无需经DNA抽提而直接用于PCR扩增  
TQ2600-01 Animal tissue Direct PCR Kit(20)                  原名为Tissue Direct PCR Kit(20) 250
TQ2600-02 Animal tissue Direct PCR Kit(100) 1100
TQ2600-03 Animal tissue Direct PCR Kit(500) 5000
TQ2610-01 Fix Direct PCR Kit(20) 350
TQ2610-02 Fix Direct PCR Kit(100) 1500
TQ2610-03 Fix Direct PCR Kit(500) 7000
石蜡组织(FFPE)直接PCR试剂盒:石蜡组织无需经DNA抽提而直接用于PCR扩增  
TQ3000-01 FFPE Direct PCR Kit(20) 300
TQ3000-02 FFPE Direct PCR Kit(100) 1300
TQ3000-03 FFPE Direct PCR Kit(500) 6000
TQ3100-01 microorganism Direct PCR Kit(20)
240
TQ3100-02 microorganism Direct PCR Kit(100)
1100
血液直接PCR试剂盒:血液无需经DNA抽提而直接用于PCR扩增  
TQ2700-01 Blood Direct PCR Kit(20) 250
TQ2700-02 Blood Direct PCR Kit(100) 1100
TQ2700-03 Blood Direct PCR Kit(500) 5000
植物组织直接PCR试剂盒:植物组织无需经DNA抽提而直接用于PCR扩增  
TQ2800-01 Plant tissue Direct PCR kit(20) 220
TQ2800-02 Plant tissue Direct PCR kit(100) 980
TQ2800-03 Plant tissue Direct PCR kit(500) 4500
植物组织直接PCR试剂盒:植物组织无需经DNA抽提而直接用于PCR扩增  
TQ2801-01 Plant tissue Direct PCR kit without Taq(20) 150
TQ2801-02 Plant tissue Direct PCR kit without Taq(100) 650
TQ2801-03 Plant tissue Direct PCR kit without Taq(500) 3000
植物种子直接PCR试剂盒:植物种子无需经DNA抽提而直接用于PCR扩增  
TQ2900-01 Plant seeds Direct PCR kit(20) 250
TQ2900-02 Plant seeds Direct PCR kit(100) 1000
TQ2900-03 Plant seeds Direct PCR kit(500) 5000
50bp DNA Ladder    
M11-01 50bp DNA Ladder(250ul) 150
M11-02 50bp DNA Ladder(1ml) 560
50bp DNA Ladder plus  
M07-01 50bp DNA Ladder Plus(250ul) 180
M07-02 50bp DNA Ladder Plus (1ml) 680
100bp DNA Ladder    
M01-01 100bp DNA Ladder(50)(即250ul) 150
M01-02 100bp DNA Ladder(200)(即1ml) 560
100bp DNA Ladder plus  
M08-01 100bp DNA Ladder Plus(250ul) 180
M08-02 100bp DNA Ladder Plus(1ml) 680
200bp DNA Ladder    
M02-01 200bp DNA ladder(50)(即250ul) 150
M02-02 200bp DNA Ladder(200)(即1ml) 560
1kb DNA Ladder    
M03-01 1kbp DNA Ladder(250ul)(即50次) 90
M03-02 1kbp DNA Ladder(1ml)(即200次) 340
1kb DNA Ladder plus  
M09-01 1Kb DNA Ladder Plus(250ul) 160
M09-02 1Kb DNA Ladder Plus(1ml) 600
DL2000 DNA Marker  
M10-01 DL2000 DNA Marker(250ul) 85
M10-02 DL2000 DNA Marker(1ml) 320
CL5000 DNA Marker  
M06-01 CL5000 DNA Marker (250ul) 150
M06-02 CL5000 DNA Marker (1ml) 560
HT DNA Marker I    
M04-01 HT Ladder I(50)(即250ul) 80
M04-02 HT Ladder I(200)(即1ml) 300
HT DNA Marker II    
M05-01 HT Ladder II (50)(即250ul) 80
M05-02 HT Ladder II (200)(即1ml) 300
     
TQR01-01 RNase inhibitor(2000U) 300
TQR01-02 RNase inhibitor(10000U) 1350
耗材与配件、溶液  
96孔真空抽滤盒    
VAC-03 EZ 96 Vacuum Manifolds 2500
柱式真空抽滤盒    
VAC-08 Vacuum Manifolds 1700
磁珠分离架    
MSD-01 E-Z 96 Magnetic Separation Device-A 1600
MSD-02 E-Z 96 Magnetic Separation Device-B 1600
MSD-03 Magnetic Separation Device-B 760
2ml收集板    
EZ9602-01 96 well 2ml collection plate(5) 250
EZ9602-02 96 well 2ml collection plate(25) 1100
500ul收集板    
EZ9604-01 96 well 500ul collection plate(5) 200
EZ9604-02 96 well 500ul collection plate(25) 900
1.2ml带盖固定圆底收集板  
SSI-1780-01 1.2ml Round Well Plate(10) 500
SSI-1780-02 1.2ml Round Well Plate(100) 3790
1.2ml带盖可拆圆底收集板  
SSI-1702-00 1.2ml 8-Strip Caps,Non-strerile,125 strips 1500
SSI-1702-S0 1.2ml 8-Strip Caps,Prestrerile,125 strips 1770
SSI-1703-00 1.2ml 8-Strip Caps,Non-strerile,80 strips 1530
SSI-1703-S0 1.2ml 8-Strip Caps,Prestrerile,805 strips 1770
SSI-1760-00 1.2ml 8-Strip tubes,Non-sterile,960 tubes 3420
SSI-1760-S0 1.2ml 8-Strip tubes,Presterile,960 tubes 3870
SSI-1770-00 1.2ml 12-Strip tubes,Non-sterile,960 tubes 3420
SSI-1770-S0 1.2ml 12-Strip Tubes,Presterile,960 tubes 3870
96孔不透气封口膜    
AC1201-01 Sealing film(50)--不透气 400
AC1201-02 Sealing film(100) 720
96孔透气封口膜    
AC1200-01 AeraSeal Film(50)--透气 400
AC1200-02 AeraSeal Film(100) 720
一次性多道移液枪分装容器  
AC1331-01 Multi-Channel Diposable reservoir(10pk) 370
AC1332-02 12-channel reservoir(5/pk.4pk/case) 850
96孔过滤板    
FL9601 E-Z 96 Lysate Clearance Plate(10) 1800
FL9602 E-Z 97 Lysate Clearance Plate(100) 12000
匀浆柱    
HCR003 Homogenizer Spin Column(200) 1000
HCR9601-02 EZ 96  Homogenization plate(4) 550
HCR9601-01 EZ 96  Homogenization plate(1) 150
Dnase试剂盒:用于RNA提取时DNA的膜上消化以去除DNA污染  
E1091-01 Rnase Free Dnase Set(50) 500
E1091-02 Rnase Free Dnase Set(200) 1800
Rnase A:(25mg/ml)在DNA制备时酶切去除RNA  
AC117 RNase A(400ul) 150
AC118 RNase A(5ml) 1050
Proteinase K:在DNA或RNA制备时酶消化样品  
AC110 Proteinase K,30mg(配buffer1.5ml) 300
AC111 Proteinase K,100mg(配buffer5ml) 780
AC112 Proteinase K,1g(配buffer50ml) 5000
OB Protease:在DNA或RNA制备时,消化样品  
AC113 OB Protease,30mg(配buffer1.5ml) 300
AC114 OB Protease,100mg(配buffer5ml) 780
溶菌酶Lysozyme:酶切消化去除细菌胞壁  
AC141 Lysozyme A(50mg\23500u/mg) 150
AC142 Lysozyme A(50mg X 4) 560
破壁酶Lyticase:酶切消化去除酵母细胞壁  
AC143 LyticaseA(2500u) 150
AC144 LyticaseA(10000u) 560
ATP-Dependent Dnase:可有效的去除质粒溶液中的基因组污染  
AC160 ATP-Dependent DNase(1000U) 1500
AC161 ATP-Dependent DNase(5000U) 2900
AC162 ATP-Dependent DNase(10000U) 4800
酸洗玻璃珠:用于物理方法裂解培养细胞,细菌或真菌细胞  
AC150 0.1mm-0.2mm Glass Beads(50g) 150
AC151 0.1mm-0.2mm Glass Beads(500g) 1050
AC152 0.4mm-0.6mm Glass Beads(50g) 150
AC153 0.4mm-0.6mm Glass Beads(500g) 1050
Oligo(dT)纤维素:用于纯化mRNA  
R6512-01 Oligo(dt)cellouse(250mg) 1480
R6512-02 Oligo(dt)cellouse(750mg) 4260
质粒相关溶液  
PS001 Solution I, 250ml 180
PS002 Solution II, 250ml 180
PS003 Solution III, 250ml 270
PS004 Neutralization Buffer, 250ml 180
PS005 Buffer SE, 250ml 180
PS006 Buffer T1, 250ml 180
PS007 Buffer T2, 250ml 180
PS008 Buffer T3, 250ml 180
PS009 Buffer HB, 250ml 315
PS010 BAC Binding Buffer,250ml 270
PS011 Buffer N3,250ml 270
PS012 ETR Reagent, 100ml 300
PS013 MGC Binding Buffer Concentrate, 20ml 270
PS014 SPM Wash Buffer Concentrate, 40 ml 270
PS015 Buffer GBT, 100 ml 270
PS016 Endotoxin-Free Elution Buffer-100ml 270
RNA相关溶液  
PR021 TRK Lysis Buffer , 100ml 900
PR022 QVL Lysis Buffer , 100ml 900
PR024 Buffer YRL, 100 ml 360
PR025 Buffer BRL, 100 ml 360
PR026 Buffer RB, 100ml 900
PR027 Buffer RPL, 100ml 900
PR028 Buffer RFL, 100ml 900
PR029 Buffer SP, 60ml 720
PR030 RNA Wash Buffer I, 100ml 360
PR031 RNA Wash Buffer II, 100ml 360
PR032 DEPC-treated Water, 100ml 120
PR033 Linear Acrylamide, 10ml 100
PR034 GTC Buffer,100ml 900
PR035 FTL Buffer,100ml 1080
PR036 AP Buffer,55ml 400
DNA、RNA回收纯化相关溶液  
PDR040 Binding Buffer, 200ml(XP2) 450
PDR041 Binding Buffer, 500ml 1080
PDR042 Buffer CP, 200ml 450
PDR043 Buffer CP, 500ml 1080
PDR044 DNA Wash Buffer Concentrate, 40ml 108
PDR045 SPW Wash Buffer Concentrate, 25ml 108
PDR046 RWB Wash Buffer Concentrate, 20ml 360
PDR047 Buffer DP, 100ml 270
PDR048 Elution Buffer, 100ml 150
PDR049 Poly-Gel DNA Elution Buffer, 100ml 180
PDR050 Buffer RFE, 100ml  270
基因组DNA相关溶液  
PD061 Buffer TL,100ml(WTL) 540
PD062 Buffer BL,100ml 900
PD063 Buffer STL,100ml 540
PD064 Buffer BDL,100ml 900
PD065 Buffer BTL,200ml 540
PD066 Buffer YL,100ml 540
PD067 Buffer MTL,100ml 540
PD068 Buffer YDL,100ml 450
PD069 Buffer ERL,100ml-红细胞裂解液 540
PD070 Buffer MSL,100ml 270
PD071 Buffer NL,100ml 270
PD072 Buffer XL,100ml 405
PD073 Buffer SP1,250ml 495
PD074 Buffer SP2, 60ml 405
PD075 Buffer SP3, 100ml 405
PD076 Buffer  P1, 250ml 495
PD077 Buffer P2, 60ml 405
PD078 Buffer P3, 100ml 405
PD079 Buffer CPL, 100ml 405
PD080 Buffer CXD, 100ml 405
PD081 Buffer SFG1, 250ml 405
PD082 Buffer SFG2, 60ml 405
PD083 Buffer SFG3, 100ml 405
PD084 Buffer FG1, 250ml 405
PD085 Buffer FG2, 60ml 455
PD086 Buffer FG3, 100ml 510
PD087 Buffer GPS  15ml 60
PD088 Buffer GPS  30ml 100
PD089 HTR 50ml 1800
PD090 Buffer SLX mlus,250ml 540
PD091 Buffer SLX ,100ml 900
PD092 Buffer DS 100ml 540
PD093 Midi Syringe 27
PD094 Max Syringe 45
PD095 ETR Binding Buffer  1L 2500
PD096 ETR Wash Buffer 1L  2100
PD097 EHB Buffer 1L 1260
SSI-1014-39 Homogenization Pestles(10/bag,20bag/cs)-0.5ml 1240
SSI-1015-39 Homogenization Pestles(10/bag,20bag/cs)-1.5ml 1240
PD098 玻璃奶 1ml 250
PD099 糖元 10mg 300
PD100 Carrier RNA 1mg 150
PD101 99.8%盐酸胍(500g) 350
PD102 99.5%异硫氰酸胍(500g) 650
代理价 单独购买柱子价格:盒子的市场价X0.7X0.55/次数 质粒、PCR、胶回收是2元/个
市场价 单独购买柱子价格:盒子的市场价X0.7/次数 质粒、PCR、胶回收是2.5元/个

 

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