表面活性剂中的BSA分析 (在线去除表面活性剂)
时间:2024-05-10 阅读:225
Sample: BSA
Pretreatment
Column : Shodex MSpak GF-4A (4.6 mm I.D. x 10 mm)
Eluent : 50 mM Sodium phosphate buffer (pH 7.0) + 150 mM NaCl
Flow rate : 1.0 mL/min
Column temp. : Room temp.
Switching time : 5 min
Analysis
Column : Shodex PROTEIN KW-804 + KW-802.5 (8.0 mm I.D. x 300 mm each)
Eluent : 50 mM Sodium phosphate buffer (pH 7.0) + 150 mM NaCl
Flow rate : 1.0 mL/min
Detector : UV (280 nm)
Column temp. : Room temp.