pSicoR载体序列
时间:2013-08-02 阅读:2917
-
提供商
上海起发实验试剂有限公司 -
资料大小
9953 -
资料图片
点击查看 -
下载次数
542次 -
资料类型
txt -
浏览次数
2917次
时间:2013-08-02 阅读:2917
提供商
上海起发实验试剂有限公司资料大小
9953资料图片
点击查看下载次数
542次资料类型
txt浏览次数
2917次型号 | 载体名称 | 出品公司 | 载体用途 |
VMA0372 | pSicoR | Addgene | 慢病毒载体 |
Backbone size (bp): 7567
Modifications to Backbone: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5': HpaI
Cloning site 3': XhoI
5' sequencing primer: mU6-F
pSicoR allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression.