pLVX-AcGFP1-N1载体说明书
时间:2013-08-02 阅读:3864
-
提供商
上海起发实验试剂有限公司 -
资料大小
144690 -
资料图片
点击查看 -
下载次数
531次 -
资料类型
pdf -
浏览次数
3864次
时间:2013-08-02 阅读:3864
提供商
上海起发实验试剂有限公司资料大小
144690资料图片
点击查看下载次数
531次资料类型
pdf浏览次数
3864次型号 | 载体名称 | 出品公司 | 载体用途 |
VMC0381 | pLVX-AcGFP1-N1 | Clontech | 慢病毒载体 |
pLVX-AcGFP1-N1 is an HIV-1-based, lentiviral expression vector that allows you to express
your gene of interest fused to AcGFP1, a green fl uorescent protein derived from Aequorea
coerulescens. Genes cloned into the multiple cloning site (MCS), located upstream of the
AcGFP1 coding sequence, are expressed as N-terminal fusions of the AcGFP1 protein.
Expression of the fusion protein is driven by the constitutively active human cytomegalovirus
immediate early promoter (P
CMV IE) located just upstream of the MCS. Lentiviral particles
derived from the vector allow the expression of AcGFP1 fusion proteins in virtually any cell
type, including primary cells. The unmodifi ed vector expresses AcGFP1, and may be used to
produce marker virus to optimize infection protocols.