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pPIC3.5K载体说明书

时间:2013-08-09      阅读:3849

 

pPIC3.5K

型号 载体名称 出品公司 载体用途
VJI0298 pPIC3.5K Invitrogen 酵母表达载体

Multiple copy integration of recombinant genes in Pichia has been demonstrated to 

increase expression of the desired gene in some cases (Brierley, et al., 1994; Clare, et al., 

1991a; Cregg, et al., 1993; Romanos, et al., 1991; Scorer, et al., 1993; Scorer, et al., 

1994; Thill, et al., 1990; Vedvick, et al., 1991). The two vectors included in this kit 

allow isolation and generation of multicopy inserts, either by in vivo or in vitro methods, 

in order to test whether increasing the copy number of your recombinant gene will lead to 

a subsequent increase in protein expression. The in vivo method utilizes hyper-resistance 

to GeneticinÆ

 (G418 sulfate) to screen for possible multicopy inserts, while the in vitro

method produces tandem inserts of your gene by ligation. 

质粒图谱: 
pPIC3.5K 质粒图谱
上海起福生物科技有限公司
:杨建辉
办公:
*8006
:
:http://www.qfbio.com/
地址:上海市浦东新区绣川路561号1102室

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