上海起发实验试剂有限公司

化工仪器网中级15

收藏

pPIC3.5K载体说明书

时间:2013-08-09      阅读:3863

 

pPIC3.5K

型号 载体名称 出品公司 载体用途
VJI0298 pPIC3.5K Invitrogen 酵母表达载体

Multiple copy integration of recombinant genes in Pichia has been demonstrated to 

increase expression of the desired gene in some cases (Brierley, et al., 1994; Clare, et al., 

1991a; Cregg, et al., 1993; Romanos, et al., 1991; Scorer, et al., 1993; Scorer, et al., 

1994; Thill, et al., 1990; Vedvick, et al., 1991). The two vectors included in this kit 

allow isolation and generation of multicopy inserts, either by in vivo or in vitro methods, 

in order to test whether increasing the copy number of your recombinant gene will lead to 

a subsequent increase in protein expression. The in vivo method utilizes hyper-resistance 

to GeneticinÆ

 (G418 sulfate) to screen for possible multicopy inserts, while the in vitro

method produces tandem inserts of your gene by ligation. 

质粒图谱: 
pPIC3.5K 质粒图谱
上海起福生物科技有限公司
:杨建辉
办公:
*8006
:
:http://www.qfbio.com/
地址:上海市浦东新区绣川路561号1102室

上一篇: pAO815载体说明书 下一篇: pPIC9K载体说明书

下载此资料需要您留下相关信息

对本公司产品近期是否有采购需求?

提示

请选择您要拨打的电话: