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pSecTag2 A&B&C说明书

时间:2013-08-09      阅读:2923

 

pSecTag2 A&B&C

型号 载体名称 出品公司 载体用途
VPI0036 pSecTag2 A Invitrogen 哺乳动物表达载体

产品参数: 

Mammalian Selection:Zeocin
Sequencing Primer:T7 Fwd
Sequencing Primer Sequence:5'd[TAATACGACTCACTATAGGG]3' 
Tag:6X His,myc

载体抗性: 
氨苄青霉素(Ampicillin)
载体描述: 

pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features:

  • Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2)
  • Cytomegalovirus (CMV) promoter for high-level constitutive expression
  • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
  • C-terminal c-myc epitope for detection with an Anti-myc Antibody
  • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7)

The pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used in E. coli.The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.

质粒图谱: 
pSecTag2 A&B&C 质粒图谱
上海起福生物科技有限公司
:杨建辉
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