Product overview
Description Rabbit polyclonal to Streptavidin （FITC）
Conjugation FITC
Conjugation notes Fluorescein isothiocyanate （FITC） （Molecular Weight 390 daltons） Absorption Wavelength: 495 nm Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Rabbit IgG
Host species Rabbit
Specificity No cross reactivity occurs against Avidin.
Tested applications Immunomicroscopy, Flow Cyt
Immunogen Streptavidin [Streptomyces avidinii]
Target
Relevance Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l.
The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in
diagnostics and laboratory kits.
Cellular localization Cytoplasmic
Alternative names SA V1 antibody
SA V2 antibody
Streptavidin V1 antibody
Streptavidin V2 antibody
Properties
Form Liquid
Storage instructions Store at +4°C short term （1-2 weeks）. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer 0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
See the website for more SDS information for this product.
Purity IgG fraction
Purification notes This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt
fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single
precipitin arc against anti-rabbit serum and streptavidin.
Clonality Polyclonal
Isotype IgG
Applications
Immunomicroscopy,
Flow Cyt
There are no notes listed as specific to these applications. For more information please see the 'general application notes' section
below or check the website.
General application notes Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays
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