Product overview
Description Mouse monoclonal [mAb51312] to CD4
Host species Mouse
Tested applications IHC-P, WB, IHC-Fr
Cross reactivity Reacts with
Mouse, Human
Immunogen Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CD4.（Note: the amino acid sequence is
proprietary）（Peptide available as ab546 99.）
Positive control This antibody gave a positive signal in Human Thymus and Human Lymph Node Tissue Lysate
Target
Function Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid
rafts.
Sequence similarities Contains 3 Ig-like C2-type （immunoglobulin-like） domains.
Contains 1 Ig-like V-type （immunoglobulin-like） domain.
Post-translational
modifications
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localization Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent
endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope
polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Target information above from: UniProt accessionP01730 The UniProt Consortium
The Universal Protein Resource （UniProt） in 2010
Nucleic Acids Res. 38:D142-D148 （2010）.
Alternative names CD 4 antibody
CD4 （L3T4） antibody
CD4 antibody
CD4 antibody
CD4 antigen （p55） antibody
CD4 Antigen antibody
CD4 molecule antibody
CD4 Receptor antibody
CD4+ Lymphocyte deficiency, included antibody
CD4_HUMAN antibody
CD4mut antibody
L3T4 antibody
Leu3 antibody
Ly-4 antibody
Lymphocyte antigen CD4 antibody
MGC165891 antibody
OTTHUMP00000238897 antibody
p55 antibody
T Cell Antigen T4 antibody
T cell antigen T4/LEU3 antibody
T cell differentiation antigen L3T4 antibody
T cell OKT4 deficiency, included antibody
http://www.abcam.com/CD4-antibody-mAb51312-ab51312.html
UK office:
Abcam plc
330 Cambridge Science Park Cambridge CB4 0FL UK
+44 （0）1223 696000
+44 （0）1223 215 215
: orders@abcam.com
US office:
Abcam Inc.
1 Kendall Square, Suite B2304 Cambridge, MA 02139-1517 USA
（888） 77-ABCAM （22226）
（877） 774-8286
These numbers are toll free in the US/Canada
: us.orders@abcam.com
Last updated on August
24, 2012
T cell surface antigen T4/Leu 3 antibody
T cell surface antigen T4/Leu3 antibody
T Cell Surface Antigen T4/Leu3 antibody
T Cell Surface Glycoprotein CD4 antibody
T-cell surface antigen T4/Leu-3 antibody
T-cell surface glycoprotein CD4 antibody
W3/25 antibody
W3/25 antigen antibody
Properties
Form Liquid
Storage instructions Store at +4°C short term （1-2 weeks）. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
See the website for more SDS information for this product.
Purity Protein G purified
Clonality Monoclonal
Clone number mAb51312
Isotype IgG1
Light chain type kappa
Applications
IHC-P IHC-P: 1/50.
WB WB: Use a concentration of 1 - 5 μg/ml. Detects a band of approximay 55 kDa （predicted molecular weight: 51 kDa）.
IHC-Fr IHC-Fr: Use at an assay dependent concentration. PubMed: 21690251
Images （See the website for higher resolution images of this product）
Our Abpromise to you: Quality guaranteed and expert technical support
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit
http://www.abcam.com/abpromise or contact our technical team.