【文献标题】Insight into the roles of packing carriers and ultrasonication in anaerobic side-stream reactor coupled membrane bioreactors: Sludge reduction performance and mechanism

【作者】Yue Zheng，Cheng Cheng，Zhen Zhou，et.al

【作者单位】上海电力大学（Shanghai University of Electric Power）

【文献中引用产品】

微生物三磷酸腺苷（ATP）ELISA试剂盒

微生物蛋白酶（Protease）试剂盒

【关键词】Sludge reduction，Anaerobic side-stream reactor，Carrier，Ultrasonication，Microbial community

【DOI】doi.org/10.1016/j.watres.2019.02.039

【影响因子(IF)】14.50

【出版期刊】《Water Research》

【产品原文引用】

ATP and protease activity were measured for samples collected from MBR, UTR and ASSR by using the ELISA kit (Shanghai Hengyuan Biotech). The sludge samples were diluted for 5 times and incubated on the enzyme plate at 37℃ for 30 min. The cycle for discarding the supernatant, injecting washing solution and incubation for 30 min was repeated for 5 times. After that, the enzyme standard reagent was added and incubated for 30 min 50 mL reagents A and B from the ELISA kit were added into the sample sequentially and mix at 37℃ for 15 min in the dark. Finally, 50 mL termination agent was added to stop the reaction and the absorbance of the samples was measured by Labsystems Multiskan MS at 450 nm in 15 min.

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