技术文献:微生物ATP合酶（ATPs)ELISA试剂盒引用文献
时间：2021-12-07 阅读：954
【文献标题】Bacterial survival strategies in sludge alkaline fermentation for volatile fatty acids production: Study on the physiological properties, temporal evolution and spatial distribution of bacterial community
【作者】Sijia Ma , Dongli Yang , Ke Xu , et.al
【作者单位】南京大学（Nanjing University）
【文献中引用产品】
微生物ATP合酶（ATPs)ELISA试剂盒 (酶活)
【关键词】Sludge alkaline fermentation ,Volatile fatty acids ,Bacterial survival strategies ,Phospholipid fatty acids ,Bacterial community distribution
【DOI】doi.org/10.1016/j.biortech.2021.125701
【影响因子(IF)】14.8
【出版期刊】《Bioresource Technology》
【产品原文引用】
2.2. Analytical methods
Total chemical oxygen demand (TCOD), SCOD (APHA 5220), total solids (TS) and VS (APHA 2540) were detected by the Standard Methods (APHA, 2005). CH4, H2 and VFAs yield were analyzed by 7890A gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) according to the method of Wang et al. (2019). COD of VFAs, CH4 and H2 was calculated based on: 1.07 g COD/g acetic acid, 1.51 g COD/g propionic acid, 1.81 g COD/g butyric acid, 2.04 g COD/g valeric acid, 1 g COD/ 382 mL CH4 and 1 g COD/ 1528 mL H2. Each reactor was run for more than 10 SRTs prior to monitoring (≥3 samples) to ensure steady state (Ma et al., 2019b) and then the samples were collected every 10 days for 30 days. For VFAs analysis, the digested sludge was centrifuged at 4000 g for 10 min, and then 0.45 μm fiber filter was used to filter the supernatant. The acidification percentage was calculated as follows: acidification percentage = (CODVFAs + CODCH4 + CODH2)/(SCOD + CODCH4 + CODH2) (Chen et al., 2017). To detect the ATP synthase activity and ATP content, the TS of the collected digestate was diluted approximately to 3000 mg/L, and then six diluted samples were collected and used for subsequent analysis. ATP synthase activity was measured using a commercial ELISA (Enzyme-Linked Immunosorbent Assay) kit (Shanghai Hengyuan Biotechnology Co., Ltd, China), and Bac Titer-GloTM (Promega, USA) was used to detect the ATP content. Experimental operations were conducted according to the manufacturer’s protocols. PLFA analysis was conducted according to the method described in Niu et al. (2012).
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