Cisbio代理
时间：2015-03-06 阅读：1712
Cisbio代理，Cisbio上海代理，Cisbio北京代理，Cisbio总代理，Cisbio一级代理，Cisbio国内代理，Cisbio中国代理
Features
- Cost effective alternative to ELISA
- Single-plate protocols for HTS
- Highly sensitive: < 39 pg/mL
- Small sample size needed: down to 5 μL
- Compatible with cell supernatant and whole cells
Applications
- Human IL5 quantification on cell supernatants and whole cells
- Functional screening of compounds using IL5 secreting cells
========================================================
Human IL5 is measured using sandwich immunoassay involving two monoclonal antibodies, one labelled with Eu-Cryptate (Donor) and the second with d2 (Acceptor). The intensity of the signal is proportional to the concentration of the IL5 present in the sample.
A Human IL5 test, like all cytokine assays, can be run with cell supernatants or with whole cells using the protocol described on the left. The antibodies labelled with HTRF? fluorophores may be pre-mixed and added in a single dispensing step to further streamline the protocol. An assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally. The Human IL5 assay protocol is described here, using a white 384-well small volume plate.
| Human IL5 | |
|---|---|
| Incubation | 3h or ON at RT |
| Linear range | 39-10,000 pg/mL |
| Detection limit | 39 pg/mL |
| extended range | 30,000 pg/mL |
As described on the figure on the right, the performances of the assay are not affected by culture media (RPMI + 10% FBS)
Description Cat. No
Human IL5 kit - 1,000 tests 63ADKPEBU007
Human IL5 kit - 20,000 tests 63ADKPECU007
订货请上海起发实验试剂有限公司
：4006551678 企业：4006551678 ：fige007
更多产品请我们
正规代理品牌：Polysciences Inc ，AB Biolabs ，abbexa ，Acris Antibodies ，arcticzymes ，AROTEC ，Athens ，Aves Labs, Inc. Bangs Laboratories ，BBI ， bellbrooklabs ，biomatik ，Biorelevant ，Biosearch ，chiralix ，Columbia Biosciences ，Crystal chem ，Dianova Enzyme Research ，FD NEURO ，FITZGRALD ，FormuMax ，Gelcompany Gene Bridges GmbH ，Glycotope，Hyglos GmbH ，iduron ，Immudex ，immunoGlob，Innovative Research ，iris-biotech ，kalenbiomed ，Ludger ，Microspheres-nanosphere ，Mybiosource ，nanocomposix ，nanotools ，Neuro Probe ，nibsc ，OLIGOENGINE ，pentapharm ，ProQinase ，prospec ，QA-Bio,Inc ，QuickZyme Biosciences ，researchdiets ，anta Cruz, springbio ，synaptic system ，TriLink BioTechnologies, Inc. ，Viagen Biotech ，Immunologic Corporation ，Zyagen Laboratories ，ZYGEM Corp Ltd，Alomone，medicago，BIOCHAIN