DSC扫描过程中缓冲液的选择
时间:2016-02-29 阅读:339
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时间:2016-02-29 阅读:339
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TA仪器资料大小
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93次资料类型
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339次Although it is often assumed that the pH of a buffer solution remains constant during a differential scanning calorimetry (DSC) experiment, the pK of a buffer can in fact change signifi cantly between 0 oC and 100 oC. Such pH changes can result in sample denaturation, aggregation and precipitation that, in the absence of knowledge about the temperature stability of the buffer, could erroneously be ascribed solely to temperature-induced unfolding of the biomolecule.