HTB-9 5637 人膀胱癌细胞

ATCC® Number:  HTB-9™        
Designations:  5637  
Depositors:   G Cannon  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens （human）  
Morphology: epithelial

HTB-9 5637 人膀胱癌细胞
Source: Organ: urinary bladder 
Tumor Stage: grade II 
Disease: carcinoma 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Isolation:  Isolation date: March, 1974 
Applications: transfection host （Roche FuGENE® Transfection Reagents） 
Tumorigenic: Yes  
DNA Profile （STR）: Amelogenin: X,Y 
CSF1PO: 11 
D13S317: 11 
D16S539: 9 
D5S818: 11,12 
D7S820: 10,11 
THO1: 7,9 
TPOX: 8,9 
vWA: 16,18 
Cytogenetic Analysis: modal chromosome number = 67; range = 59 to 71The stemline modal chromosome number is 67 occurring at 36%, and polyploidy at 0.6%. Fourteen marker chromosomes were common to these cells: 3q+, 11q+, i（13q）, t（9q21q）, i（17q）, i（21q） and 8 others of unknown origin. Additional markers: der（5）t（5;7）（q31;p11） and 1p were found that were specific to a minor subpopulation. Microchromosomes （larger than double minutes） and double minutes （DM） were also found in a small population of cells. Normal N13 was absent; and N21 was found only in the minor subpopulation.One, or occasionally two, Y chromosomes were present in each cell. 
Isoenzymes:  AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 1-2 
Me-2, 1 
PGM1, 1 
PGM3, 2
Age:  68 years adult  
Gender:  male  
Ethnicity:  Caucasian  
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C

Subculturing:  Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended 
Medium Renewal: 2 to 3 times per week 
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5% HTB-9 5637