CRL-1932 786-O 人肾透明细胞腺癌细胞

ATCC® Number:  CRL-1932™     
Designations:  786-O [786-0]  
Depositors:   RD Williams  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens （human）  
Morphology: epithelial

 CRL-1932 786-O 人肾透明细胞腺癌细胞
Source: Organ: kidney 
Disease: renal cell adenocarcinoma 
Cellular Products: parathyroid hormone （PTH） like peptide  
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CRL-1932  
Applications: transfection host （technology from amaxa） 
Tumorigenic: Yes  
DNA Profile （STR）: Amelogenin: X,Y 
CSF1PO: 10 
D13S317: 8 
D16S539: 12 
D5S818: 9 
D7S820: 11,12 
THO1: 6,9.3 
TPOX: 8,11 
vWA: 15,17 
Cytogenetic Analysis: hypertriploid; Y was present in 60% the cells examined 
Age:  58 years  
Gender:  male  
Ethnicity:  Caucasian  
Comments: This line was derived from a primary clear cell adenocarcinoma. 
The cells display both microvilli and desmosomes, and can be grown in soft agar. 
The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. 
The peptide has an N terminal sequence similar to PTH, has PTH like activity, and has a molecular weight of 6000 daltons. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing:  Protocol: 
CRL-1932
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

CRL-1932 

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:12 is recommended 
Medium Renewal: Every 2 to 3 days 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001
recommended serum:ATCC 30-2020 
References: 22648: Williams RD, et al. In vitro c*tion of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528
22653: Williams RD, et al. In vitro c*tion of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102 
CRL-1932 

CRL-1932