CCL-119 CCRF-CEM 人急性淋巴细胞白血病T淋巴细胞

ATCC® Number:  CCL-119™      
Designations:  CCRF-CEM [CCRF CEM]  
Depositors:   GE Foley  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: suspension 
Organism: Homo sapiens （human）  
Morphology: lymphoblast

CCL-119 CCRF-CEM 人急性淋巴细胞白血病T淋巴细胞 
Source: Organ: peripheral blood 
Disease: acute lymphoblastic leukemia 
Cell Type: T lymphoblast; 
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CCL-119 CCRF-CEM 人急性淋巴细胞白血病T淋巴细胞 
Isolation:  Isolation date: November, 1964 
Applications: transfection host （Nucleofection technology from Lonza） 
Tumorigenic: Yes  
Reverse Transcript: negative  
Antigen Expression: CD3; Homo sapiens, expressed 
CD4; Homo sapiens, expressed 
CD5; Homo sapiens, expressed 
CD7; Homo sapiens, expressed 
DNA Profile （STR）: Amelogenin: X 
CSF1PO: 10,11 
D13S317: 11,12 
D16S539: 10,13 
D5S818: 12,13 
D7S820: 9,13 
THO1: 6,7 
TPOX: 8 
vWA: 17,19 
Cytogenetic Analysis: modal number = 47; range = 41 to 95. 
Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted. 
Isoenzymes:  ADA, 1 
ES-D, 1 
G6PD, B 
GLO-I, 1 
PEP-D, 1 
PGD, C 
PGM1, 1 
PGM3, 0 
Age:  4 years juvenile  
Gender:  female  
Ethnicity:  Caucasian  
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing:  Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cell density between 2 to 3 X 10 exp5 and 1 to 2 X 10 exp6 viable cells/ml. 
Medium Renewal: Add fresh medium （20% to 30% by volume） every 2 to 3 days 
Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-2264
derivative:ATCC CRL-2265
derivative:ATCC CRL-8993
derivative:ATCC TIB-195 
References: 22788: Foley GE, et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965. PubMed: 14278051
23337: Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323
25967: Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381
26284: Uzman BG, et al. Morphologic variations in human leukemic lymphoblasts （CCRF-CEM cells） after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. PubMed: 5224274
26293: Adams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047
32913: Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 
CCL-119 CCRF-CEM 人急性淋巴细胞白血病T淋巴细胞 
CCL-119 CCRF-CEM 人急性淋巴细胞白血病T淋巴细胞