HTB-36 JEG-3 人绒毛膜癌细胞

ATCC® Number:  HTB-36™        
Designations:  JEG-3  
Depositors:   G Kohler  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens （human）  
Morphology: epithelial

ATCC®编号：HTB-36™
名称：JEG-3
存款人：G Kohler
生物安全等级：1
发货：冷冻
中度和血清：见传播
成长属性：坚持
生物：智人（人类）
形态学：上皮细胞

HTB-36 JEG-3 人绒毛膜癌细胞 
Source: Organ: placenta 
Disease: choriocarcinoma 
Cellular Products: human chorionic gonadotropin （hCG）, human chorionic somatomammotropin （placental lactogen）; progesterone  
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HTB-36  
Applications: transfection host （Roche FuGENE® Transfection Reagents） 
Tumorigenic: Yes  
DNA Profile （STR）: Amelogenin: X,Y 
CSF1PO: 11,12 
D13S317: 9,11 
D16S539: 13,14 
D5S818: 10,11 
D7S820: 10,12 
THO1: 9,9.3 
TPOX: 8 
vWA: 16 
Cytogenetic Analysis: This is a hypertriploid human cell line. The modal chromosome number is 71, occurring at 34%, and polyploidy at 2.6%. The t（4;11）（p15;q13）, i（13q）, t（10p15q）, del（18）（q21）, and 6 other markers are common to most cells, and two other markers are found in some. Giant salites are seen in one N14, and two N22. N2, N5, and N9 have 4 copies, and N7, N13, N18, N21 and X a single copy. A single Y chromosome is detected by Q-band examination. 
Isoenzymes:  AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 1-2 
PGM1, 1 
PGM3, 1-2 
Comments: This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates. 
The cells are able to transform steroid precursors to estrone and estradiol 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing:  Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended 
Medium Renewal: 2 to 3 times per week 
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. 
Add fresh culture medium, aspirate and dispense into new culture flasks. 
Preservation:  Freeze medium: Culture medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
recommended serum:ATCC 30-2020 
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23377: . . Acta Endocrinol. Suppl. 153: 137-153, 1971.
32288: Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029
32564: Roesler WJ, et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491
58051: Kohler PO, Bridson WE. Isolation of hormone-producing clonal lines of human choriocarcinoma. J. Clin. Endocrinol. 32: 683-687, 1971. 
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