CCL-246 KG-1 人白血病细胞

ATCC® Number:  CCL-246™       
Designations:  KG-1  
Depositors:   DW Golde  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: suspension 
Organism: Homo sapiens （human）  
Morphology: myeloblast

CCL-246 KG-1 人白血病细胞
Source: Organ: bone marrow 
Disease: acute myelogenous leukemia 
Cellular Products: HLA DR  
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CCL-246  
Applications: transfection host （technology from amaxa） 
Reverse Transcript: negative  
Antigen Expression: HLA A30, A31, B35, Cw4 
DNA Profile （STR）: Amelogenin: X,Y 
CSF1PO: 7 
D13S317: 11,12 
D16S539: 10,11 
D5S818: 13 
D7S820: 8,10 
THO1: 7,8 
TPOX: 7,9 
vWA: 14,19 
Cytogenetic Analysis: The stemline chromosome number is near-diploid, with the 2S component occurring at 1.8%. Five markers （constitutive markers） were common to most, if not all, metaphases analyzed. Modal chromosome number is 47 （or 46 plus a small metacentric chromosome which is smaller than the G1 group chromosome）. Normal chromosomes 5, 7, 8, 12 and 17 were monosomic, and others were disomic. The Y chromosome is detected in the Q-banded preparations. 
Isoenzymes:  AK-1, 0 
ES-D, 1 
G6PD, B 
GLO-I, 2 
Me-2, 1 
PGM1, 1-2 
PGM3, 0 
Age:  59 years  
Gender:  male  
Ethnicity:  Caucasian  
Comments: The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde. A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia. [1056] 
KG-1 cells spontaneously differentiate to granulocyte and macrophage like cells. 
They show a good response to colony stimulating factor （CSF）. [1056] 
The line is EBNA negative （EBNA-）. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C 
Subculturing:  Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 （5） viable cells/ml. Maintain cell density between 2 X 10（5） and 1 X 10（6） viable cells/ml. Do not allow to exceed 2 X 10（6） cells/ml. 
Medium Renewal: Twice per week 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2005
recommended serum:ATCC 30-2020 
References: 867: Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765
1056: Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682
26069: . . Blood 54: 174a, 1979.
33055: Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689 
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CCL-246

CCL-246