美国National Diagnostics公司

SequaGel MD 测序SequaGel  突变检测胶
 

• Point Mutation Analysis
• SSCP Analysis
• Heteroduplex Analysis

Catalog Number: EC-845
 

1.用于检测微小突变差异的产品.

2.适用于点突变分析.

3.适用于单链构象多态性分析（SSCP Analysis）.

4.适用于异源双链分析 （Heteroduplex Analysis）.
 

得益于相对迁移率的高分辨率，SequaGelTM突变检测胶允许发现DNA序列中的微小突变差异。SequaGelTM突变检测胶采用保护的配方，提供2X浓度的贮存液用于单链构象多态性和异源双链的分析。异源双链试剂盒由SequaGelTM突变检测胶溶液和三色上样缓冲液组成，单链构象多态性试剂盒由SequaGelTM突变检测胶溶液和SSCP终止液组成。100-300bp的SSCP核酸样品建议使用0.5X浓度的SequaGelTM突变检测胶，更小的片段则建议使用更高浓度（0.75X）的胶，比较大的片段则可以使用较低浓度（0.4X）的胶。异源双链的核酸样品在不超过900bp时均推荐使用1X浓度的胶。
 

SequaGel MD permits minor mutational differences in DNA sequences to be detected as a high resolution relative mobility （Rf） shift. SequaGel MD is a proprietary formulation, supplied as a 2X stock, designed to resolve sequence related differences by SSCP （Single Strand Conformational Polymorphism） and Heteroduplex Analysis.

DNA mutations, or sequence modifications, are readily associated with specific disease states. Since a variation of a single nucleotide in a sequence may indicate a significant genetic anomaly, an extremely sensitive method to analyze these mutations is necessary.

Two principle methods of conformational analysis have been developed, SSCP Analysis and Heteroduplex Analysis. SequaGel MD is available as a monomer solution packaged with Triple Dye Loading Buffer in the SequaGel MD Heteroduplex Kit or with SSCP Stop Solution in the SequaGel MD SSCP Kit.

SequaGel MD is designed to run SSCP samples in the 100-300 nucleotide range at a 0.5X concentration. Small fragments may be better resolved by using higher concentration （0.75X） gels. Larger fragments may be better resolved by running lower concentration （0.4X） gels. SequaGel MD is designed to run heteroduplex analysis on DNA fragments up to 900 bases at a 1X concentration.