PH7.2磷酸盐缓冲液配制方法
时间：2013-03-09 阅读：4986
Microbiologics公司的部分菌株产品有自带的稀释液，有些就需要自制的PH7.2磷酸盐缓冲液，实验室也有很多地方会用到该缓冲液，其配制方法如下：
Stock Solution— Dissolve 34 g of monobasic potassium phosphate in about 500 mL of water contained in a 1000-mL volumetric flask. Adjust to pH 7.2 ± 0.1 by the addition of sodium hydroxide TS （about 175 mL）, add water to volume, and mix. Dispense and sterilize. Store under refrigeration.
For use, dilute the Stock Solution with water in the ratio of 1 to 800, and sterilize.