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反转录酶III 逆转录酶三 invitrogen正品原装 SuperScript® III Reverse Transcriptase
货号18080-044
与SuperScriptII逆转录酶一样，SuperScriptIII的RNase H 活性较低，能获得更高产量的全长Cdna。另外，它还有着其它的逆转录酶所无法提供的热稳定性；经过基因工程改造的SuperScriptIII具有更高的热稳定性，其半衰期在50度时达到令人难以置信的220分钟，这个特性也提高SuperScriptIII转录具有二级结构模板RNA的能力。
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invitrogen：www.invitrogen.com
产品描述
SuperScript® III Reverse Transcriptase （RT） is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers （GSPs） and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly （A）+RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.
•Thermostability– Half life of 220 minutes at 50°C
•Yield– Reduced RNase H activity for more full-length cDNA
•Specificity– Full activity at 50°C for increased specificity with Gene Specific Primers
•Applications– Array labeling, cDNA libraries, RT-PCR, primer extension, and 3´ and 5´ RACE
Source: Purified fromE. coliexpressing thepolgene of M-MLV （1, 2）, mutagenized to increase thermal stability and half life and reduce RNase H activity.
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays （Figure 2） and yield and length of cDNA product.
Unit Definition: One unit of SuperScript® III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly（A）•oligo（dT）12-18as a template•primer （3）.
Unit Reaction Conditions: 50 mM Tris-HCl （pH 8.3）, 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly（A）, 0.1 mM oligo（dT）12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
