Speficity：    Goat polyclonal IgG，affinity purified by Protein A.

             Reacts with： Due to the conserved nature of the target reactivity would be predicted in wide range of species.

Immunogen: 8-Hydroxyguanosine - conjugate.

Positive control ：Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37℃

Target:       8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.

Application:   IHC-P: 1/200.

             ELISA: 1/100000 - 1/250000.

ICC/IF: Use at an assay dependent dilution.  PubMed: 16678792

not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end use

上海安研，供应8 Hydroxyguanosine抗体 相关的抗原

8 Hydroxyguanosine抗原

8 Hydroxyguanosine antigen