BioXcell：InVivoMAb anti-mouse CSF1R (CD115)
时间：2022-08-01 阅读：532
一、 产品介绍
落刺激因子1受体(CSF1R)，也称为巨噬细胞集落刺激因子受体(M-CSFR)，即CD115反应。CSF1R是一种单程I型膜蛋白，是血小板衍生生长因子受体家族的成员。在小鼠中，CSF1R由单核细胞/巨噬细胞、腹膜渗出物细胞、浆细胞样和常规树突细胞以及破骨细胞表达。CSF1R是CSF1的受体，CSF1R通过CSF1R调节单核细胞谱系中细胞的增殖和分化。据报道，克隆号AFS98抗体可在体内deplete巨噬细胞并阻断CSFR1。
二 、产品详情
| 产品详情 | |
| 产品货号 | BE0213 |
| 产品规格 | 1/5/25/50/100mg |
| 抗体亚型 | Rat IgG2a, κ |
| 推荐同型对照 | InVivoMAb rat IgG2a isotype control, anti-trinitrophenol（货号：BE0089） |
| 推荐抗体稀释液 | InVivoPure™ pH 7.0 Dilution Buffer（货号：IP0070） |
| 免疫原 | Not available or unknown |
| 应用 | in vivo macrophage depletion/in vitro CSF1R neutralization/in vivo monocyte depletion/Flow cytometry/Western blot |
| 产品形式 | PBS , pH 7.0 Contains no stabilizers or preservatives |
| 内毒素水平 | <2EU/mg (<0.002EU/μg) 使用 LAL gel clotting 测定 |
| 纯度 | >95% Determined by SDS-PAGE |
| 无菌处理 | 0.2 μM filtered |
| 生产形式 | 从组织培养上清液中纯化得到。 |
| 纯化形式 | Protein G |
| RRID | AB_2687699 |
| 分子量大小 | 150 kDa |
| 保存条件 | 抗体原溶液应保存在4°C条件下，不要冷冻保存。 |
三 、已发表文献
| 用途 | 已发表文献 |
| in vivo macrophage depletion | Bauche, D., et al. (2018). “LAG3(+) Regulatory T Cells Restrain Interleukin-23-Producing CX3CR1(+) Gut-Resident Macrophages during Group 3 Innate Lymphoid Cell-Driven Colitis.” Immunity 49(2): 342-352 e345 |
| in vivo macrophage depletion | Gordon, S. R., et al. (2017). “PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity.” Nature 545(7655): 495-499 |
| in vivo macrophage depletion | Moynihan, K. D., et al. (2016). “Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses.” Nat Med. doi: 10.1038/nm.4200 |
| in vivo macrophage depletion | Arnold, I. C., et al. (2015). “CD11c monocyte/macrophages promote chronic Helicobacter hepaticus-induced intestinal inflammation through the production of IL-23.” Mucosal Immunol. doi: 10.1038/mi.2015.65 |
| in vivo macrophage depletion | Kaminsky, L. W., et al. (2015). “Redundant Function of Plasmacytoid and Conventional Dendritic Cells Is Required To Survive a Natural Virus Infection.” J Virol 89(19): 9974-9985 |
| in vivo monocyte depletion | Naik, S., et al. (2015). “Commensal-dendritic-cell interaction specifies a unique protective skin immune signature.” Nature 520(7545): 104-108 |
| in vitro CSF-R1 neutralization | Sheng, K. C., et al. (2014). “IL-3 and CSF-1 interact to promote generation of CD11c+ IL-10-producing macrophages.” PLoS One 9(4): e95208. |
| in vivo monocyte depletion | Greter, M., et al. (2012). “GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cells.” Immunity 36(6): 1031-1046. |
| in vivo macrophage depletion | Tagliani, E., et al. (2011). “Coordinate regulation of tissue macrophage and dendritic cell population dynamics by CSF-1.” J Exp Med 208(9): 1901-1916 |
| in vivo macrophage depletion | Lim, A. K., et al. (2009). “Antibody blockade of c-fms suppresses the progression of inflammation and injury in early diabetic nephropathy in obese db/db mice.” Diabetologia 52(8): 1669-1679. |
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