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CHO HCP ELISA kit, 3G

    This CHO HCP 3rd Generation ELISA kit uses a new and even more broadly reactive antibody than our earlier kits. The kit incorporates a number of other improvements in the detection of CHO HCPs. This new ELISA is more sensitive than the previous kits, able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO. It has been extensively qualified with a large number of drug substances and in-process samples. This data indicates the kit is truly a generic method that can likely be validated as not only a process development tool, but in most cases as a lot release test, eliminating the need to develop a custom or process specific assay. The antibody has been evaluated for reactivity to individual HCPs using the conventional 2D Western Blot as well as a method we have shown to be superior to 2D WB, which we call 2D HPLC-ELISA. This 2D HPLC-ELISA method demonstrated the antibody is reactive to more than 750 individual HCPs from conditioned media and cell lysates in both a CHO-S strain as well as a K1 strain. These 750+ HCPs represent more than 98% of the total mass of protein as indicated by protein detection methods orthogonal to ELISA. As such, this antibody may overcome concerns about missing any significant individual HCPs. This new ELISA is also more robust than the earlier kits showing less sample matrix interference and improved dilutional linearity. If you have found the F015 and CM015 kits lacking in any way meeting your HCP detection needs, or if you are considering the need for a process-specific assay, we recommend you evaluate this 3rd Generation CHO HCP ELISA kit.

    Host Cells used for recombinant expression are complex systems containing hundreds to thousands of host cell proteins (HCPs) that can contaminate biopharmaceutical products. Failure to sufficiently remove contaminants early in drug development can result in reduced drug efficacy or adverse patient reactions.

    Regulations require HCPs be reduced to the lowest levels possible. Our generic ELISA kits are useful in both process development and process validation. Using these kits in the earliest phase of product development can provide relevant and specific data directing process improvement and decreasing the levels of HCP contamination.

    In quality control applications, subject to thorough validation and qualification by each laboratory, these kits may also be useful for lot release testing.

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Product   Catalog #   Description

A549 HCP ELISA kit    F230       Host cell protein assay, 96 tests

A549 HCP Western Blot Kit  F235       Host cell protein assay, 5 mini blots

BHK HCP ELISA Kit    F510       Host cell protein assay, 96 tests

Bovine IgG ELISA Kit  F070       Bioprocess contaminant assay, 96 tests

Bovine Plasma Protein ELISA Kit F290       Bioprocess contaminant assay, 96 tests

BSA ELISA Kit     F030       Bioprocess contaminant assay, 96 tests

Bovine Transferrin ELISA Kit      F120       Bioprocess contaminant assay, 96 tests

CHO HCP ELISA Kit    F015       Host cell protein assay, 96 tests

CHO HCP Western Blot Kit   F060       Host cell protein assay, 5 mini blots

CHO-CM ELISA Kit     CM015    Host cell protein assay, 96 tests

CHO-CM Western Blot Kit    CM060    Host cell protein assay, 5 mini blots

CHO HCP ELISA Kit, 3G     F550       Host cell protein assay, 96 tests

E. coli HCP ELISA Kit        F410       Host cell protein assay, 96 tests

E. coli HCP Western Blot Kit       F415       Host cell protein assay, 5 mini blots

Goat Casein HCP ELISA Kit F420       Host cell protein assay, 96 tests

Goat IgG ELISA Kit      F210       Bioprocess contaminant assay, 96 tests

Total Goat Milk Proteins ELISA kit      F240       Host cell protein assay, 96 tests

Total Goat Milk Proteins WB kit   F245       Host cell protein assay, 5 mini blots

Human Albumin ELISA Kit  F055       Bioprocess contaminant assay, 96 tests

Human IgA ELISA Kit F165       Bioprocess contaminant assay, 96 tests

Human IgG-total ELISA Kit        F160       Bioprocess contaminant assay, 96 tests

Human IgM ELISA Kit        F170       Bioprocess contaminant assay, 96 tests

HEK 293 HCP ELISA Kit     F650       Host cell protein assay, 96 tests

Human Transferrin ELISA Kit      F035N    Bioprocess contaminant assay, 96 tests

Insect Cell SF9 HCP ELISA Kit    F020       Host cell protein assay, 96 tests

Insect Cell SF9 Western Blot Kit   F125       Host cell protein assay, 5 mini blots

Insulin ELISA Kit  F040       Bioprocess contaminant assay, 96 tests

Lactococcus lactic HCP ELISA Kit       F490       Host cell protein assay, 96 tests

Lactococcus lactic HCP WB Kit    F495       Host cell protein assay, 5 mini blots

Mouse Total IgG ELISA Kit  F049       Bioprocess contaminant assay, 96 tests

Mouse IgG1 ELISA Kit F045       Bioprocess contaminant assay, 96 tests

Mouse IgG2a ELISA Kit       F046       Bioprocess contaminant assay, 96 tests

Mouse IgG2b ELISA Kit       F047       Bioprocess contaminant assay, 96 tests

Mouse IgM ELISA Kit   F090       Bioprocess contaminant assay, 96 tests

MRC5 HCP ELISA Kit  F300       Host cell protein assay, 96 tests

NS/0 HCP ELISA kit     F220       Host cell protein assay, 96 tests

NS/0 HCP Western Blot kit   F225       Host cell protein assay, 5 mini blots

PER.C6 cell line HCP ELISA Kit  F530       Host cell protein assay, 96 tests

Pichia pastoris HCP ELISA Kit     F140       Host cell protein assay, 96 tests

P.pastoris HCP Western Blot Kit    F145       Host cell protein assay, 5 mini blots

Pichia pastoris HCP ELISA Kit, 2nd Generation  F640       Antibodies to glycosylation epitopes have been removed and is much more specific than the F140.

Protein A ELISA Kit      F050       Bioprocess contaminant assay, 96 tests

Protein A ELISA for human IgG   F050H    Bioprocess contaminant assay, 96 tests

Protein A ELISA kit      F400       Bioprocess contaminant assay, 96 tests

Ps. fluorescens HCP ELISA kit     F450       Host cell protein assay, 96 tests

S. cerevisiae HCP ELISA Kit F135       Host cell protein assay, 96 tests

S. cerevisiae HCP Western Blot Kit      F130       Western Blot assay, 5 mini blots

SP 2/0 HCP ELISA Kit  F180       Host cell protein assay, 96 tests

SP 2/0 HCP Western Blot Kit F185       Western Blot assay, 5 mini blots

S.aureus HCP ELISA Kit      F320       Host cell protein assay, 96 tests

Ultra-Sensitive Insulin ELISA Kit F280       Bioprocess contaminant assay, 96 tests

Vero cell HCP ELISA kit       F500       Host cell protein assay, 96 tests

Polyclonal Antisera       

A549 HCP      A549      Goat, neat serum

A549 HCP      A549-PA Goat, IgG fraction

A549 HCP      A549-AF Goat, affinity purified

Bovine Plasma Proteins  BPP04    Rabbit, neat serum

Bovine Plasma Proteins  BPP04-PA      Rabbit, IgG fraction

Bovine Plasma Proteins  BPP04-AF      Rabbit, affinity purified

BHK HCP      BHK1107       Goat, neat serum

BHK HCP      BHK1107-PA  Goat, IgG fraction

BHK HCP      BHK1107-AF Goat, affinity purified

Campylobacter species   9-25B-PA       Rabbit, IgG fraction

Campylobacter species   9-61A     Guinea Pig, neat serum

Chlamydia species  8-29A     Rabbit, neat serum

Chlamydia species  8-29A-PA       Rabbit, IgG fraction

Chlamydia species  8-48 Guinea Pig, neat serum

CHO HCP      C0016     Goat, neat serum

CHO HCP      C0016-PA      Goat, IgG fraction

CHO HCP      C0016-AF      Goat, affinity purified

CHO-CM HCP       M0016    Goat, neat serum

CHO-CM HCP       M0016-PA      Goat, IgG fraction

CHO-CM HCP       M0016-AF     Goat, affinity purified

CHO HCP 3G 3G-0016  Goat, neat serum

CHO HCP 3G 3G-0016PA    Goat, IgG fraction

CHO HCP 3G 3G-0016AF    Goat, affinity purified

Cytomegalovirus    8-21B     Rabbit, neat serum

Cytomegalovirus    8-53A     Guinea Pig, neat serum

E. coli HCP    F117       Goat, neat serum

E. coli HCP    F117-PA  Goat, IgG fraction

E. coli HCP    AP117     Goat, affinity purified

Haemophilus influenzae 8-21A     Rabbit, neat serum

Haemophilus influenzae 8-21A-PA       Rabbit, IgG fraction

Haemophilus influenzae 8-53B     Guinea Pig, neat serum

Haemophilus influenzae 8-53B-PA       Guinea Pig, IgG fraction

HEK 293 HCP       HK293    Goat, neat serum

Herpes Simplex Virus    8-25A     Rabbit, neat serum

Herpes Simplex Virus    8-25A-PA       Rabbit, IgG fraction

Herpes Simplex Virus    8-40B     Guinea Pig, neat serum

Klebsiella pneumoniae, Genus      9-01 Rabbit, neat serum

Lactococcus lactic HCP  LL5557   Goat, neat serum

Lactococcus lactic HCP  LL5557-PA     Goat, IgG fraction

Lactococcus lactic HCP  LL5557-AF    Goat, affinity purified

MRC5 HCP    MRC5     Goat, neat serum

MRC5 HCP    MRC5-PA      Goat, IgG fraction

MRC5 HCP    MRC5-AF      Goat, affinity purified

Mycobacterium genus    8-27B     Rabbit, neat serum

Mycobacterium genus    1-04-PA  Guinea Pig, IgG fraction

Neisseria meningitidis    8-24B     Rabbit, neat serum

Neisseria meningitidis    8-36 Guinea Pig, neat serum

NS/0 HCP      N9192     Goat, neat serum

NS/0 HCP      N9192-PA      Goat, IgG fraction

NS/0 HCP      N9192-AF      Goat, affinity purified

PER.C6 cell line HCP    PC118     Goat, neat serum

PER.C6 cell line HCP    PC118-PG      Goat, IgG fraction

PER.C6 cell line HCP    PC118-AF      Goat, affinity purified

Pichia pastoris HCP       PI-49      Goat, neat serum

Pichia pastoris HCP       PI-49PA  Goat, IgG fraction

Pichia pastoris HCP       PI-49AF  Goat, affinity purified

Pichia pastoris HCP       PI-49AF-B     Goat, affinity purified-biotinylated

Pichia pastoris HCP       PI-49AF-AP   Goat, affinity purified-alk. phosphatase

Pseudomonas fluorescens      7-04 Goat, neat serum

Pseudomonas fluorescens      7-04PA   Goat, IgG fraction

Pseudomonas fluorescens      7-04AF   Goat, affinity purified

Pseudomonas species     8-57 Guinea Pig, neat serum

Saccharomyces cerevisiae HCP     82-SC     Goat, neat serum

Saccharomyces cerevisiae HCP     82SC-PA Goat, IgG fraction

Saccharomyces cerevisiae HCP     82SC-AF Goat, affinity purified

Shigella species      9-03-PA  Rabbit, IgG fraction

Shigella species      9-04 Rabbit, neat serum

SF9 HCP 12-SF9    Rabbit, neat serum

SF9 HCP 12-SF9-PA     Rabbit, IgG fraction

SF21 HCP      34-SF21  Rabbit, neat serum

SF21 HCP      34-SF21-PA   Rabbit, IgG fraction

SP2/0 HCP     SP110     Goat, neat serum

SP2/0 HCP     SP110-PA       Goat, IgG fraction

SP2/0 HCP     SP110-AF      Goat, affinity purified

SP2/0 HCP     SP110AF-B    Goat, affinity purified-biotinylated

SP2/0 HCP     SP110AF-AP  Goat, affinity purified-alk. phosphatase

S. aureus HCP       SA8114 Goat, neat serum

S. aureus HCP       SA8114-AF    Goat, affinity purified

Streptococcus pneumoniae     8-31B     Rabbit, neat serum

Streptococcus pneumoniae     8-42A     Guinea Pig, neat serum

Streptococcus pneumoniae     8-43A-PA       Guinea Pig, IgG fraction

Total Goat Milk Proteins       4042       Rabbit, neat serum

Total Goat Milk Proteins       4042-PA  Rabbit, IgG fraction

Total Goat Milk Proteins       4042-AF Rabbit, affinity purified

Vero cell HCP VC807    Goat, neat serum

Vero cell HCP VC807-PA      Goat, IgG fraction

Vero cell HCP VC807-AF     Goat, affinity purified

Stab-ELISA-rÔ Enzyme Conjugate Diluent/Stabilizers             

Alkaline Phosphatase    I-045      500mL

Alkaline Phosphatase    I-040      1000mL

Alk Phos w/ HAMA blocker  I-045H    500mL

Alk Phos w/ HAMA blocker  I-040H    1000mL

Horseradish Peroxidase  I-035      500mL

Horseradish Peroxidase  I-030      1000mL

HRP w/ HAMA blocker I-035H    500mL

HRP w/ HAMA blocker I-030H    1000mL

Liquid Substrates          

PNPP Liquid Substrate   F008       For use with Alk Phos in ELISA, 100mL

PNPP Liquid Substrate   F10008   For use with Alk Phos in ELISA, 1L

TMB Liquid Substrate    F027       For use with HRP in ELISA, 100mL

TMB Liquid Substrate    F100027  For use with HRP in ELISA, 1L

TMB Liquid Substrate    F129       For use with HRP in Western Blot, 100mL

TMB Liquid Substrate    F100029  For use with HRP in Western Blot, 1L

BCIP/NBT Liquid Substrate  F064       For use with Alk Phos in WB, 1 reagent, 100mL

BCIP/NBT Liquid Substrate  F00064   For use with Alk Phos in WB, 1 reagent, 1L

Sample Dilution Buffers             

Sample Dilution Buffer, 1L， 100mL, 500mL    I028    I028-100   I028-500    For use with BHK, E.coli, CHO, CHO-CM, Human IgG's, HSA, hTransferrin, HEK 293, Insulin, L.lactic, Mouse IgG's, Pichia pastoris, S.cerevisiae, Protein A, Vero cell ELISA kits

A549 Sample Diluent, 100mL  , 500mL     F233A-100     F233A-500 For use with A549 HCP ELISA kit

BSA Sample Dilution Buffer, 1L   , 100mL  , 500mL     F031A  / F031A-100 / F031A-500      For use with BSA, Bovine IgG, Bovine Plasma Proteins and Bovine Transferrin ELISA kits

Goat IgG Dilution Buffer, 1L    , 100mL   , 500mL       F213A  /F213A-100 / F213A-500       For use with Goat  IgG ELISA kit

Goat Milk Diluent Buffer, 1L     , 100mL  r, 500mL      F243A  /F243A-100 /F243A-500 For use with Goat Milk Proteins ELISA kit

NS/0 Sample Dilution Buffer, 1L ，100mL   ，500mL   F223A  / F223A-100 /F223A-500       For use with NS/0 HCP ELISA kit

SP2/0 Sample Dilution Buffer, 1L , 100mL   , 500mL      F183A  / F183A-100 / F183A-500      For use with SP2/0 HCP ELISA kit

Immunogenicity Products            

Passive Coated Immunogenicity Kit     IM10      Kit for immunogenicity testing

Biotinylated Antigen Coated Immunogenicity Kit       IM20      Kit for immunogenicity testing

Biotinylation Kit    IM30     

Human Immunoglobulin Screening Kit IM40      Kit for immunogenicity testing

Human IgG Subclass Kit       IM50      Kit for immunogenicity testing

Substrate Kit   IM109     TMB & Stop Solution for ELISA, 50mL

Coating Buffer, 1L IM101     For immunogenicity testing

Conjugate Diluent, 1L    IM103     For immunogenicity testing

Conjugate Diluent, 500mL    IM103-500     For immunogenicity testing

Sample Diluent, 500mL IM104     For immunogenicity testing

FG Blocker, 1L      IM107     For immunogenicity testing

BSA Antigen, 1mg/mL   IM112     For immunogenicity testing

Bovine IgG Antigen, 1mg/mL       IM113     For immunogenicity testing

Bovine Transferrin Antigen, 1mg/mL   IM114     For immunogenicity testing

CHO HCP Antigen, 1mg/mL IM115     For immunogenicity testing

E. coli HCP Antigen, 1mg/mL      IM116     For immunogenicity testing

Goat IgG Antigen, 1mg/mL   IM117     For immunogenicity testing

HSA Antigen, 1mg/mL  IM118     For immunogenicity testing

Human IgD Antigen, 1mg/mL      IM141     For immunogenicity testing

Human Transferrin Antigen, 1mg/mL   IM119     For immunogenicity testing

Insulin Antigen, 1mg/mL      IM121     For immunogenicity testing

Mouse IgG Antigen, 1mg/mL       IM122     For immunogenicity testing

NS/0 HCP Antigen, 1mg/mL IM123     For immunogenicity testing

SP2/0 HCP Antigen, 1mg/mL       IM125     For immunogenicity testing

Gyros Kits-discontinued       

CHO 3G HCP G550      For use on the Gyros xP workstation, 560 tests

CHO 3G HCP G550-1   For use on the Gyros xP workstation, 112 tests

E.coli HCP     G410      For use on the Gyros xP workstation, 560 tests

E.coli HCP     G410-1   For use on the Gyros xP workstation, 112 tests

Protein A       G400      For use on the Gyros xP workstation, 560 tests

Protein A       G400-1   For use on the Gyros xP workstation, 112 tests

DNA Kits             

DNA Extracton in Wells D100W   96 tests in a deep well microtiter plate

DNA Extraction in Tubes      D100T    50 tests in microfuge tubes

Residual CHO Host Cell  kit in Wells  D550W   96 tests in a deep well microtiter plate

Residual CHO Host Cell kit in Tubes    D550T    50 tests in microfuge tubes

CHO Amplification kit in Wells    D555W   96 tests in a deep well microtiter plate

CHO Amplification kit in Tubes    D555T    50 tests in microfuge tubes

Residual Ecoli Host Cell  kit in Wells  D410W   96 tests in a deep well microtiter plate

Residual Ecoli Host Cell  kit in Tubes D410T    50 tests in microfuge tubes

E.coli Amplification kit in Wells   D415W   96 tests in a deep well microtiter plate

E.coli Amplification kit in Tubes  D415T    50 tests in microfuge tubes

Residual NS/0 Host Cell  kit in Wells  D220W   96 tests in a deep well microtiter plate

Residual NS/0 Host Cell kit in Tubes    D220T    50 tests in microfuge tubes

Miscellaneous Reagents & Components       

Deep Well Extraction Plate w/Sealing Mat   D102      5 plates

DNA Extraction Buffer for use in tubes       D108      30mL

DNA Extraction Buffer for use in wells       D105      30mL

DNA Sample Diluent     D006-100       100mL

DNA Sample Diluent     D006-500       500mL

DNA Sample Diluent     D006-1000     1L

DNA TE Buffer, 1L       D001      1L

CHO DNA Concentrate       D552      10ug/mL, 1mL

E.coli DNA Concentrate      D412      10ug/mL, 1mL

NS/0 DNA Concentrate       D222      10ug/mL, 1mL

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