from Vibrio cholerae
别名: Cholera enterotoxin, Cholergen
CAS号: 9012-63-9
MDL number: MFCD00130776
包装
Package size based on protein content.
Reconstitution
When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
Unit Definition
One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthine （IBMX）.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA
Biochem/physiol Actions
Toxin consisting of an A subunit （27 kDa） surrounded by five B subunits （approximay 12 kDa each）, which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein （Gαs）, reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.
相关类型 Bacteria, Bioactive Small Molecules, C-CH, Cell Biology, Cell Signaling and Neuroscience,
G Protein Function, G Proteins and Cyclic Nucleotides, Reagents, Toxins and Venoms
Quality Level PREMIUM
assay ~95% （SDS-PAGE）
form lyophilized powder
composition Protein, ~5% Lowry-TCA
solubility H2O: soluble10 mg/mL
storage temp. 2-8°C |
