Rat Insulin EIA 大鼠胰岛素检测试剂盒背肾景介绍:

Mercodia Rat Insulin ELISA provides a method for the quantitative determination of insulin in rat serum or plasma.

Rat Insulin EIA 大鼠胰岛素检测试剂盒Summary and explanation of the test

Insulin is the principal hormone responsible for the control of glucose metabolism.It is synthesised in the ß-cells of the islets of Langerhans as the precursor,proinsulin, which is processed to form C-peptide and insulin. Both are secretedin equimolar amounts into the portal circulation. The mature insulin moleculecomprises two polypeptide chains, the A chain and the B chain. The two chainsare linked together by two inter-chain disulphide bridges. There is also an intrachain disulphide bridge in the A chain. Secretion of insulin is mainly controlled by plasma glucose concentration,and the hormone has a number of important metabolic actions. Its principalfunction is to control the uptake and utilisation of glucose in peripheral tissuesvia the glucose transporter. This and other hypoglycaemic activities, such as theinhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by thehyperglycaemic hormones including glucagon, epinephrine (adrenaline), growth hormone and cortisol.

Rat Insulin EIA 大鼠胰岛素检测试剂盒Principle of the procedure

Mercodia Rat Insulin ELISA is a solid phase two-site enzyme immunoassay. It isbased on the direct sandwich technique in which two monoclonal antibodies aredirected against separate antigenic determinants on the insulin molecule. Duringincubation insulin in the sample reacts with peroxidase-conjugated anti-insulinantibodies and anti-insulin antibodies bound to micro-titer well. A simple washingstep removes unbound enzyme labeled antibody. The bound conjugate is detectedby reaction with 3,3’,5,5’-tetramethylbenzidine. The reaction is stopped by addingacid to give a colorimetric endpoint that is read spectrophotometrically.