基于SYBR Green的实时定量PCR依旧是当前多数研究人员所依赖的价格相对低廉、的基因表达测定方法，RealTimePrimers致力于为科研工作者提供价格低廉，经高度优化的实时定量PCR引物系列,结合高质量的模板和试剂，RealTimePrimers产品将为您呈现清晰、的PCR实验结果。现今，越来越多的研究者选择了RealTimePrimers产品。
 实时定量PCR产物的产物定量有两种方式：使用已知浓度质粒DNA或对照基因的标准曲线来定量，和相对简单的相对定量法。两者都要保证扩增效率一致，但通常情况下，纯靶标产物的扩增效率要高于情况复杂的混合物样品，在实验中尤其要注意避免酚污染和(对RNA反转后定量)DNA污染等问题。
相对定量方法通过管家基因或对照基因来归一化靶标基因，是更常用的定量方式。在这一过程中选择合适的对照基因十分重要，通常需要一系列管家基因，以便综合或是找到稳定的基因来完成这一工作。
 RealTimePrimers产品介绍：
1 信号通路PCR arrays (Pathway PCR arrays)
将信号通路上的基因集合形成arrays，一块微板上含88个靶标基因外加8个管家基因(housekeeping gene)，大幅度降低了实验费用，提高了实验效率。
已有信号通路产品如下：

以人继发和先天免疫反应引物系列(Human Adaptive & Innate Immune Response Primer Library)为例，简单给予描述：
信号通路PCR arrays以96孔微板形式提供，每板含88个引物对和8个管家基因引物对，只需加入cDNA模板和SYBR green master mix即可实验。这一人继发和先天免疫反应引物系列所含基因如下：
ADORA2A Adenosine A2a receptor
C5 Complement component 5
C8A Complement component 8, alpha polypeptide
CAMP Cathelicidin antimicrobial peptide
CASP1 Caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
CASP4 Caspase 4, apoptosis-related cysteine peptidase
CCL2 Chemokine (C-C motif) ligand 2
CCR3 Chemokine (C-C motif) receptor 3
CD14 CD14 molecule
CD1D CD1d molecule
CD55 CD55 molecule, decay accelerating factor for complement (Cromer blood group)
CHUK Conserved helix-loop-helix ubiquitous kinase
COLEC12 Collectin sub-family member 12
CRP C-reactive protein, pentraxin-related
CXCR4 Chemokine (C-X-C motif) receptor 4
CYBB Cytochrome b-245, beta polypeptide
DEFB4 Defensin, beta 4
DMBT1 Deleted in malignant brain tumors 1
FN1 Fibronectin 1
HMOX1 Heme oxygenase (decycling) 1
IFNA1 Interferon, alpha 1
IFNB1 Interferon, beta 1, fibroblast
IFNG Interferon Gamma
IFNGR1 Interferon gamma receptor 1
IFNGR2 Interferon gamma receptor 2 (interferon gamma transducer 1)
IKBKB Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
IL10 Interleukin 10
IL12RB2 Interleukin 12 receptor, beta 2
IL1A Interleukin 1, alpha
IL1B Interleukin 1, beta
IL1F10 Interleukin 1 family, member 10 (theta)
IL1R1 Interleukin 1 receptor, type I
IL1R2 Interleukin 1 receptor, type II
IL1RAP Interleukin 1 receptor accessory protein
IL1RAPL2 Interleukin 1 receptor accessory protein-like 2
IL1RL2 Interleukin 1 receptor-like 2
IL1RN Interleukin 1 receptor antagonist
IL2 Interleukin 2
IL36RN Interleukin 36 receptor antagonist
IL36A Interleukin 36, alpha
IL37 Interleukin 37
IL36B Interleukin 36, beta
IL36G Interleukin 36, gamma
IL4 Interleukin 4
IL6 Interleukin 6 (interferon, beta 2)
IL8 Interleukin 8
IRAK1 Interleukin-1 receptor-associated kinase 1
IRAK2 Interleukin-1 receptor-associated kinase 2
IRF1 Interferon regulatory factor 1
LALBA Lactalbumin, alpha-
LBP Lipopolysaccharide binding protein
LTF Lactotransferrin
LY96 Lymphocyte antigen 96
LYZ Lysozyme
MAPK14 Mitogen-activated protein kinase 14
MAPK8 Mitogen-activated protein kinase 8
MIF Macrophage migration inhibitory factor (glycosylation-inhibiting factor)
MYD88 Myeloid differentiation primary response gene (88)
NCF4 Neutrophil cytosolic factor 4, 40kDa
NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
NFKB2 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
NFKBIA Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
NLRC4 NLR family, CARD domain containing 4
NOS2 Nitric oxide synthase 2, inducible
PGLYRP1 Peptidoglycan recognition protein 1
PGLYRP2 Peptidoglycan recognition protein 2
PGLYRP3 Peptidoglycan recognition protein 3
PPBP Pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
PROC Protein C (inactivator of coagulation factors Va and VIIIa)
PTAFR Platelet-activating factor receptor S100A12 S100 calcium binding protein A12
SERPINA1 Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
SERPINE1 Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
SFTPD Surfactant protein D
TGFB1 Transforming growth factor, beta 1
TLR1 Toll-like receptor 1
TLR10 Toll-like receptor 10
TLR2 Toll-like receptor 2
TLR3 Toll-like receptor 3
TLR4 Toll-like receptor 4
TLR6 Toll-like receptor 6
TLR8 Toll-like receptor 8
TLR9 Toll-like receptor 9
TNF Tumor necrosis factor
TNFRSF1A Tumor necrosis factor receptor superfamily, member 1A
TOLLIP Toll interacting protein
TRAF6 TNF receptor-associated factor 6
TREM1 Triggering receptor expressed on myeloid cells 1
ACTB Actin, beta
B2M Beta-2-microglobulin
GAPDH Glyceraldehyde-3-phosphate dehydrogenase
GUSB Glucuronidase, beta
HPRT1 Hypoxanthine phosphoribosyltransferase 1
Pgk1 Phosphoglycerate kinase 1
PPIA Peptidylprolyl isomerase A
RPL13A Ribosomal protein L13a

2. 实时定量PCR引物
Realtimeprimers提供您所研究基因经证实的引物系列，并提供引物序列信息和详细的反应条件，可满足1000次PCR反应所需。
这类引物系列包括人、小鼠和大鼠三个物种。

以人PCR primer sets为例，包括：

3.提供管家基因引物序列信息，够1000次反应使用。通过比较多个看家基因表达，更好定量靶标基因的水平。
这类引物系列包括人、小鼠和大鼠三个物种。

以人housekeeping基因系列为例，包括基因：ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, RPL13A, TBP和TFRC。

4. 基因家族(gene family)引物序列
将基因家族的成员汇总，提供已证实的引物序列，用于研究不同成员的表达差异，并提供引物的序列信息。
这类基因家族包括：

以人TNF基因家族为例，成员包括：TNF, LTA, LTB, TNFSF4, TNFSF5, TNFSF6, TNFSF7, TNFSF8, TNFSF9, TNFSF10, TNFSF11, TNFSF13, TNFSF13B, TNFSF14, TNFSF15, TNFSF18等
 

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