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英文名称：Cellartis GS1‐R Rat pluripotent stem cell culture medium

中文名称：Cellartis GS1‐R大鼠多能干细胞培养基

用途：大鼠ES和EG细胞的分离培养和长期维持生殖潜力

保存：基础培养基-20ºC；添加剂-80ºC

GS1‐R是Cellartis推出的一款专门用于大鼠（Rats）胚胎干细胞（ES）或胚胎生殖细胞（EG）的培养基。该产品中的成分可以压抑细胞分化信号，从而很好地把培养的细胞保持在未分化的基态（ground state）,与此同时细胞的活性和增殖不受影响。在本产品中培养增殖的大鼠细胞可以用于向生殖细胞发育相关的研究潜力，可以用来构造转基因大鼠，比如通过传统的homologous recombination方法或是 nuclease-based gene editing方法。GS1‐R是成分确定的培养基，无血清添加，需要饲养层。

GS1‐R is a compley defined, serum‐free cell culture medium that is used for the derivation, maintenance, and propagation of rat embryonic stem (ES) and embryonic germ (EG) cells. The medium contains selective small molecule inhibitors that block differentiation-inducing signals, maintaining cells in the ‘ground state’ of self-renewal and promoting cell survival and growth (1–3). Rat ES cells cultured in GS1‐R medium are germ-line competent and can be used to generate transgenic knock‐out rats using either classic homologous recombination or nuclease-based gene editing techniques.

产品特点

成分*确定的配方；无血清

利用选择性小分子抑制剂阻断来自GSK3β和ERK / MEK的分化信号

在该培养基中培养可以有效地扩增大鼠ES细胞并进行遗传操作

Compley defined formulation; serum-free

Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK

Culture in this medium allows rat ES cells to be efficiently derived, propagated, and genetically manipulated

产品应用(ES：胚胎干细胞；EG：胚胎生殖细胞）

大鼠ES和EG细胞的分离培养和长期维持生殖潜力

Derivation and long‐term maintenance of germline-competent rat ES and EG cells

组份：培养基+添加剂

100 ml medium

100 µl 2i inhibitor supplement

自备试剂：饲养层细胞或预铺培养容器+LIF

Rat ES and EG cells will require co-culture support from feeder cells; Stem Cell Sciences (UK) Ltd. recommends inactivated mouse embryonic fibroblasts seeded at 2 - 4 x 104 cells/cm2 on pre-gelatinised culture vessels.Further supplementation with an activating STAT3 signalling cytokine such as LIF is optional5.

大鼠ES和EG细胞需要来自饲养细胞的共培养支持; Stem Cell Sciences（UK）Ltd.推荐灭活的小鼠胚胎成纤维细胞接种2-4×10 ⁴个细胞/ cm ²在pre-gelatinised的培养容器上。进一步补充活化STAT3信号传导因子如LIF。

保存：Upon receipt, store the media at -20℃ and the 2i supplement at -80℃ until ready to use.When stored under these conditions, the products are stable for 12 months from the date of manufacture (see label).Once thawed and combined, store at 4℃ and use within 2 weeks.This product is light sensitive, and should be protected from light.

参考文献：

1. Li P, et al. (2008) Germline competent embryonic stem cells derived from rat blastocysts. Cell. 135(7): 1299-1310.

2.Buehr M, et al. (2008) Capture of authentic embryonic stem cells from rat blastocysts. Cell. 135(7): 1287-1298.

3.Leitch H, et al. (2013) Naive pluripotency is associated with global DNA hypo-methylation. Nat Struct Mol Biol. 20: 311–316.

4.Tong C, et al. (2011) Generating gene knockout rats by homologous recom-bination in embryonic stem cells. Nat Protocols. 6: 827-844.

5.Blair K, et al. (2012) Culture parameters for stable expansion, genetic modi-fication and germline transmission of rat pluripotent stem cells. Biol Open.1(1): 58-65.

6.Tong C, et al. (2012) Rapid and cost-effective gene targeting in rat embryonic stem cells by TALENs. J Genet Genomics. 39(6): 275-280.

Cellartis 小鼠/大鼠胚胎干细胞培养基系列：成分确定、无血清培养基

GS2-M® 小鼠胚胎干细胞和多能诱导干细胞培养基，无饲养层，不依赖LIF

iSTEM™ 小鼠胚胎干细胞培养基，难获得ES细胞小鼠

GS1-R® 大鼠多能干细胞培养基，需要饲养层，胚胎干细胞和胚胎生殖细胞（EG）

华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司主营细胞治疗和精准医疗等产品，2018年与TAKARA继续合作，授权代理（上海）其旗下Cellartis干细胞研究制品和Rubicon单细胞文库构建试剂。Cellartis公司位于瑞典，拥有iPS细胞等干细胞分化为肝细胞及脏器细胞的分化诱导技术和ES细胞，ips细胞，分化细胞等干细胞相关产品。

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