2023年2月，西部特色生物资源保护与利用教育部重点实验室;宁夏大学生命科学学院；宁夏银川市贺兰山路(Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Yinchuan 750021, Ningxia, China；School of Life Sciences, Ningxia University, 539 W.  Helanshan Road, Yinchuan 750021, Ningxia, China) Zhanyou Liu老师研究团队在《Scientifc Reports》上发表论文：

“DUSP1 mediates BCG induced apoptosis and inflammatory response in THP-1 cells via MAPKs/NF-κB signaling pathway”

“DUSP1通过MAPKs/NF-κB信号通路介导BCG诱导的THP-1细胞凋亡和炎症反应”

Abstract：

Tuberculosis (TB) is a zoonotic infectious disease caused by Mycobacterium tuberculosis (Mtb). Apoptosis and necrosis caused by the interaction between the host and the pathogen, as well as the host's inflammatory response, play an important role in the pathogenesis of TB. Dual-specificity phosphatase 1 (DUSP1) plays a vital role in regulating the host immune responses. However, the role of DUSP1 in the regulation of THP-1 macrophage apoptosis induced by attenuated Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection remains unclear. In the present study, we report that infection with BCG significantly induces macrophage apoptosis and induces the production of DUSP1, TNF-α and IL-1β. DUSP1 knockdown significantly inhibited BCG-induced macrophage apoptosis and activation of MAPKs/NF-κB signaling pathway. In addition, DUSP1 knockdown suppressed BCG-induced inflammation in vivo. Taken together, this study demonstrates that DUSP1, as a regulator of MAPKs/NF-κB signaling pathway, plays a novel role in BCG-induced macrophage apoptosis and inflammatory response.

摘要：

结核病(TB)是由结核分枝杆菌(Mtb)引起的人畜共患传染病。宿主与病原体相互作用引起的细胞凋亡和坏死以及宿主的炎症反应在TB的发病机制中起着重要作用。双特异性磷酸酶1 (DUSP1)在调节宿主免疫应答中起着至关重要的作用。然而，DUSP1在弱毒牛分枝杆菌卡介苗感染诱导的THP-1巨噬细胞凋亡中的作用尚不清楚。在本研究中，我们报道了卡介苗感染可显著诱导巨噬细胞凋亡并诱导DUSP1、TNF-α和IL-1β的产生。DUSP1基因敲低可显著抑制bcg诱导的巨噬细胞凋亡和MAPKs/NF-κB信号通路的激活。此外，DUSP1敲低可抑制bcg诱导的体内炎症。综上所述，本研究表明DUSP1作为MAPKs/NF-κB信号通路的调节因子，在bcg诱导的巨噬细胞凋亡和炎症反应中发挥了新的作用。

该论文中，人髓系白血病单核细胞THP-1细胞的体外培养是使用Ausbian特级胎牛血清完成的。