一、 转染小鼠胚胎成纤维细胞（NIH-3T3），转染siRNA文章概述：

当NIH/3T3细胞生长密度达到60%，在不含FBS培养基的六孔板中进行转染。对于细胞转染使用5μM pGPU6/GFP/Neo-shTRIM11,siALKBH5,miR-21a-5p mimic,miR-590–5p mimic, miR-361–3p mimic, miR-202–3p mimic和相应对照，使用 Advanced DNA/RNA转染试剂(#AD600025 Zeta life, USA) 对细胞转染；在转染24小时后分别对TRIM11或ALKBH5通过蛋白质印迹或qPCR检测敲低效率。

二、其它

1、NIH/3T3细胞培养条件：文章选择Z7181FBS-500胎牛血清培养(# Zeta life, USA)

2、文章作者：河北医科大学公共卫生学院毒理学教研室、河北医科大学环境与健康重点实验室

3、标题：FcγRIIB The proteasome-dependent degradation of ALKBH5 regulates ECM deposition in PM2.5 exposure-induced pulmonary fibrosis of mice .(IF10.588)

三、转染转染NIH-3T3细胞原文

Cell transfection

Transfection was conducted when NIH/3T3 cells grown to 60% density in six-well plates with FBS absent medium. For cell transfection, 5 μM of pGPU6/GFP/Neo-shTRIM11, siALKBH5, miR-21a-5p mimic, miR-590–5p mimic, miR-361–3p mimic, miR-202–3p mimic and cor-responding control (Genepharma, Shanghai, China) was transfected into cells using Advanced DNA/RNA Transfection Reagent (Zeta life, USA) according to the supplier’s guidelines, respectively. TRIM11 or ALKBH5 knockdown efficiency was detected by western blotting or qPCR after 24 h post-transfection, respectively.

四、美国Zeta Life 公司与美国加利福尼亚大学旧金山校区联合开发用于哺乳动物细胞、活体动物转染的 Advanced DNA RNA 第三代多肽小分子转染试剂，此技术成为新的蛋白功能、免疫细胞及干细胞治疗、研发及生产的主要关键技术之一。

五、产品信息