illumina/美国因美纳 品牌
生产厂家厂商性质
上海市所在地
Feto SDS-PAGE蛋白快速染液(免脱色)
¥315蛋白染液
¥165MAGBIO HighPrep PCR AC-60050
¥6500Applikon Z110005011 电极液
¥850易汇Viskase,5m干型透析袋44-8000-14000D
面议4404335 POROS XS 强阳离子交换树脂
¥250000resDNASEQ™ 定量 CHO DNA 试剂盒
面议Illumina MiSeq Reagent Kit v3
面议EaivellyPCR板
面议EaivellyPCR八联管0.2ml
面议Eaivelly血清移液管
面议Eaivelly细胞培养板
面议illumina NXTR XT样本制备试剂盒 *
illumina NXTR XT样本制备试剂盒 *
illumina 15032350
illumina 15032350 NXTR XT样本制备试剂盒
NXTR XT样本制备试剂盒(24样本 第一盒)
Nextera XT Library Prep kit 96 Samples(BOX 1of2) 15032354
NXTR XT标签试剂盒V2(套件A 96标签 192样本)15052163
Next-Generation sequencing
Total RNA was extracted from each patient’s plasma sample, and cDNA synthesis followed. The QIAamp Viral RNA Mini Kit (Cat. #52904, Qiagen, Gaithersburg, MD) and the SuperScript�6�4 IV First-Strand Synthesis System (Cat. # 18091050, Invitrogen, Carlsbad, CA) were used respectively and according to manufacturers’ instructions. Multiple sets of HIV-1 specific primer pairs were used to target and amplify using polymerase-chain-reaction (PCR) the protease (PR), reverse transcriptase (RT), integrase (int), and envelope (env) HIV-1 genes (~43% of genome)36. Library preparation was completed with Nextera XT Library Prep (Cat. # 15032350, Illumina, Dan Diego, CA). Samples were then sequenced on eight runs on an Illumina MiSeq platform using the MiSeq v2 (300 cycles) chemistry (Cat. # MS-102–2002, Illumina). Both library prep and sequencing were completed according to the manufacturer’s instructions. All DNA sequence files are available from the GenBank database under SRA accession: PRJNA517147.